Information on EC 1.13.11.39 - biphenyl-2,3-diol 1,2-dioxygenase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
1.13.11.39
-
RECOMMENDED NAME
GeneOntology No.
biphenyl-2,3-diol 1,2-dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
also acts on 3-isopropylcatechol, forming 7-methyl-2-hydroxy-6-oxoocta-2,4-dienoate. Not identical with EC1.13.11.2 catechol 2,3-dioxygenase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dioxygenation
-
-
-
-
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
2-hydroxybiphenyl degradation
-
biphenyl degradation
-
carbazole degradation
-
Chlorocyclohexane and chlorobenzene degradation
-
Dioxin degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
biphenyl-2,3-diol:oxygen 1,2-oxidoreductase (decyclizing)
Contains Fe2+ or Mn2+ [3]. This enzyme participates in the degradation pathway of biphenyl and PCB (poly chlorinated biphenyls), and catalyses the first ring cleavage step by incorporating two oxygen atoms into the catechol ring formed by EC 1.3.1.56, cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase. The enzyme from the bacterium Burkholderia xenovorans LB400 can also process catechol, 3-methylcatechol, and 4-methylcatechol, but less efficiently [1]. The enzyme from the carbazole-degrader Pseudomonas resinovorans strain CA10 also accepts 2'-aminobiphenyl-2,3-diol [5]. The enzyme from Ralstonia sp. SBUG 290 can also accept 1,2-dihydroxydibenzofuran and 1,2-dihydroxynaphthalene [4]. The enzyme is strongly inhibited by the substrate [1]. Not identical with EC 1.13.11.2 catechol 2,3-dioxygenase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2'-aminobiphenyl-2,3-diol 1,2-dioxygenase
-
-
2'-aminobiphenyl-2,3-diol 1,2-dioxygenase
Pseudomonas resinovorans CA10
-
-
-
2,3-DBPD
-
-
2,3-DBPD
Pseudomonas putida KF715, Pseudomonas putida PpG7
-
-
-
2,3-DHBD
Q52032
-
2,3-DHBD
Pseudomonas putida OU83
Q52032
;
-
2,3-DHBD
-
-
-
2,3-DHBD
Q53126
-
2,3-DHBD
Rhodococcus sp. RHA1
Q53126
-
-
2,3-DHBP dioxygenase
Q8GR45
-
2,3-DHBP dioxygenase
Q8GR45
-
-
2,3-DHBP dioxygenase
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-, B3VTK1, B3VTL5
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-, B3VTK1, B3VTL5
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
A9QT35
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
;
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
A9QT35
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl 1,2-dioxygenase
F2X5L9
-
2,3-dihydroxybiphenyl 1,2-dioxygenases
Q6QF56, Q6QF57
-
2,3-dihydroxybiphenyl 1,2-dioxygenases
Q6QF56, Q6QF57
-
-
2,3-dihydroxybiphenyl dioxygenase
-
-
-
-
2,3-dihydroxybiphenyl dioxygenase
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
Q8GR45
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
Q8GR45
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
Ralstonia sp. SBUG
-
-
-
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
-
2,3-dihydroxybiphenyl:oxygen 1,2 oxidoreductase
-
-
2,3-dihydroxybiphenyl:oxygen 1,2 oxidoreductase
-
-
-
2,3-diOH-biphenyl 1,2-dioxygenase
-
-
2,3-diOH-biphenyl 1,2-dioxygenase
Ralstonia sp. SBUG
-
-
-
2-aminobiphenyl-2,3-diol-1,2-dioxygenase
-
-
23 OHBP oxygnase
-
-
23 OHBP oxygnase
-
-
-
23 OHBP oxygnase
-
-
23 OHBP oxygnase
Pseudomonas putida KF715
-
-
-
3-phenylcatechol dioxygenase
Q52032
-
3-phenylcatechol dioxygenase
Pseudomonas putida OU83
Q52032
-
-
biphenyl-2,3-diol dioxygenase
-
-
-
-
BPDO
Comamonas testosteroni B-356
-
-
-
BphC
-, B3VTK1, B3VTL5
-
BphC
-, B3VTK1, B3VTL5
-
-
BphC
-
-
BphC
-
-
-
BphC
-
gene name
BphC
A9QT35
-
BphC
-
gene name
-
BphC
A9QT35
-
-
BphC
Ralstonia sp. SBUG
-
-
-
BphC
F2X5L9
gene name
BphC1
B3VTK1
gene name
BphC1
B3VTK1
gene name
-
BphC1
Rhodococcus globerulus P6
-
-
-
BphC1
Q6QF57
-
BphC1
Q6QF57
-
-
BphC1-BN6
-
-
BphC1-BN6
Sphingomonas sp. BN6
-
-
-
BphC2
B3VTL5
gene name
BphC2
B3VTL5
gene name
-
BphC2
Rhodococcus globerulus P6
-
-
-
BphC2
Q6QF56
-
BphC2
Q6QF56
-
-
BphC2-BN6
-
-
BphC2-BN6
Sphingomonas sp. BN6
-
-
-
BphC3
-
gene name
BphC3
-
gene name
-
BphC3
Rhodococcus globerulus P6
-
-
-
BphCLA-4
-
-
-
BphC_JF8
Q8GR45
-
BphC_JF8
Q8GR45
-
-
BphC_LA-4
-
-
BphC_LA-4
-
-
-
CarB
Pseudomonas resinovorans CA10
-
-
-
CarBaBb
Pseudomonas resinovorans CA10
-
-
-
DHBD
Burkholderia sp. LB400
-
-
-
EtbC
Q53126
strain RDC1, bphC gene mutant
EtbC
Rhodococcus sp. RHA1
Q53126
strain RDC1, bphC gene mutant
-
manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
-
manganese(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
103679-58-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
BphC1; isolated from petroleum-contaminated soil, gene bphC1
UniProt
Manually annotated by BRENDA team
BphC2; isolated from petroleum-contaminated soil, gene bphC2
UniProt
Manually annotated by BRENDA team
isolated from petroleum-contaminated soil, gene bphC3
-
-
Manually annotated by BRENDA team
BphC1; isolated from petroleum-contaminated soil, gene bphC1
UniProt
Manually annotated by BRENDA team
BphC2; isolated from petroleum-contaminated soil, gene bphC2
UniProt
Manually annotated by BRENDA team
isolated from petroleum-contaminated soil, gene bphC3
-
-
Manually annotated by BRENDA team
gene BsbphCI
SwissProt
Manually annotated by BRENDA team
JF8
SwissProt
Manually annotated by BRENDA team
gene BsbphCI
SwissProt
Manually annotated by BRENDA team
organism reclassified, prior refered as Pseudomonas sp. strain LB400 or Pseudomonas cepacia strain LB400
-
-
Manually annotated by BRENDA team
Burkholderia cepacia LB400
strain LB400
-
-
Manually annotated by BRENDA team
Burkholderia sp. LB400
LB400
-
-
Manually annotated by BRENDA team
strain LB400
-
-
Manually annotated by BRENDA team
Comamonas sp. SMN4
SMN4
-
-
Manually annotated by BRENDA team
formerly Pseudomonas testosteroni strain B-356
-
-
Manually annotated by BRENDA team
strain B-356
-
-
Manually annotated by BRENDA team
Comamonas testosteroni B-356
strain B-356
-
-
Manually annotated by BRENDA team
strain H850
-
-
Manually annotated by BRENDA team
Cupriavidus necator H850
strain H850
-
-
Manually annotated by BRENDA team
gene bphC
-
-
Manually annotated by BRENDA team
strain BCG
-
-
Manually annotated by BRENDA team
native enzyme and expressed in Pseudomonas aeruginosa
-
-
Manually annotated by BRENDA team
strain KF707; strain KF714
-
-
Manually annotated by BRENDA team
Pseudomonas pseudoalcaligenes KF714
strain KF714
-
-
Manually annotated by BRENDA team
strain F1
-
-
Manually annotated by BRENDA team
strain F1; strain KF715
-
-
Manually annotated by BRENDA team
strain F1; strain KF715; strain PpG7
-
-
Manually annotated by BRENDA team
strain KF715
-
-
Manually annotated by BRENDA team
strain KT2442
-
-
Manually annotated by BRENDA team
strain OU83, EMBL nucleotide database
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida KF715
strain KF715
-
-
Manually annotated by BRENDA team
Pseudomonas putida KT2442
strain KT2442
-
-
Manually annotated by BRENDA team
Pseudomonas putida OU83
strain OU83, EMBL nucleotide database
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida PpG7
strain PpG7
-
-
Manually annotated by BRENDA team
Pseudomonas resinovorans CA10
-
-
-
Manually annotated by BRENDA team
strain CB406
-
-
Manually annotated by BRENDA team
strain DJ-12; strain KKS102
-
-
Manually annotated by BRENDA team
strain DJ77; strain KKS102
-
-
Manually annotated by BRENDA team
strain IC; strain KKS102
-
-
Manually annotated by BRENDA team
Pseudomonas sp. CB406
strain CB406
-
-
Manually annotated by BRENDA team
strain DJ-12
-
-
Manually annotated by BRENDA team
Pseudomonas sp. DJ77
strain DJ77
-
-
Manually annotated by BRENDA team
strain IC
-
-
Manually annotated by BRENDA team
ATCC 31258
-
-
Manually annotated by BRENDA team
strain SBUG 290
-
-
Manually annotated by BRENDA team
Ralstonia sp. SBUG
strain SBUG 290
-
-
Manually annotated by BRENDA team
strain TA421
-
-
Manually annotated by BRENDA team
Rhodococcus erythropolis TA421
strain TA421
-
-
Manually annotated by BRENDA team
formerly Acinetobacter sp. strain P6
-
-
Manually annotated by BRENDA team
Rhodococcus globerulus P6
strain P6
-
-
Manually annotated by BRENDA team
fragment; strain R04
SwissProt
Manually annotated by BRENDA team
strain R04
SwissProt
Manually annotated by BRENDA team
strain RDC1 derived from strain RHA1, bphC gene mutant
-
-
Manually annotated by BRENDA team
strain RHA1
SwissProt
Manually annotated by BRENDA team
fragment; strain R04
SwissProt
Manually annotated by BRENDA team
strain R04
SwissProt
Manually annotated by BRENDA team
Rhodococcus sp. RHA1
strain RHA1
SwissProt
Manually annotated by BRENDA team
formerly Pseudomonas paucimobilis strain Q1
-
-
Manually annotated by BRENDA team
formerly Pseudomonas paucimobilis strain Q1; strain B1, formerly Beijerincka sp. wild-type strain B1
-
-
Manually annotated by BRENDA team
Sphingobium yanoikuyae Q1
strain Q1
-
-
Manually annotated by BRENDA team
strain BN6
-
-
Manually annotated by BRENDA team
Sphingomonas sp. BN6
strain BN6
-
-
Manually annotated by BRENDA team
activated sludge metagenome, gene bphC_meta
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the 2,3-DHBP dioxygenase is a key enzyme in the polychlorinated biphenyl degradation pathway
metabolism
F2X5L9
BphC is the key enzyme for polychlorobiphenyl transformation
metabolism
-, B3VTK1, B3VTL5
BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview; BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview; BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview
physiological function
-, Q8GR45
2,3-dihydroxybiphenyl-1,2-dioxygenase plays an important role in the degradation of polychlorinated biphenyls
physiological function
F2X5L9
BphC catalyzes the ring cleavage of 2,3-dihydroxybiphenyl during biphenyl/polychlorobipheny degradation process
physiological function
-
2,3-dihydroxybiphenyl-1,2-dioxygenase plays an important role in the degradation of polychlorinated biphenyls
-
metabolism
-
BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview; BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview; BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview
-
additional information
-
modeling of substrates into the active site of the enzyme using BphC from Burkholderia xenovorans strain LB400, which has 74% identity, as the templates, molecular docking studies, overview
additional information
-
analysis of the interaction between BphC_LA-4 and catecholic substrates by homology modeling and molecular docking, model of the BphC_LA-4-catechol complex, overview. BphC_LA-4 presents lower binding affinity towards 4-methylcatechol in comparison with 3-methylcatechol and catechol. A BphC_LA-4 enzyme electrode prepared by SiO2 sol-gel shows good response to all these three catecholic compounds
additional information
F2X5L9
enzyme homology modeling, overview
additional information
-
analysis of the interaction between BphC_LA-4 and catecholic substrates by homology modeling and molecular docking, model of the BphC_LA-4-catechol complex, overview. BphC_LA-4 presents lower binding affinity towards 4-methylcatechol in comparison with 3-methylcatechol and catechol. A BphC_LA-4 enzyme electrode prepared by SiO2 sol-gel shows good response to all these three catecholic compounds; modeling of substrates into the active site of the enzyme using BphC from Burkholderia xenovorans strain LB400, which has 74% identity, as the templates, molecular docking studies, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dihydroxydibenzofuran + O2
?
show the reaction diagram
-
-
-
-
?
1,2-dihydroxynaphthalene + O2
?
show the reaction diagram
-
-
-
-
?
2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus, Rhodococcus globerulus P6
-
49% of activity with 2,3-dihydroxybiphenyl
-
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
25% of activity with 2,3-dihydroxybiphenyl, 57% of activity with 2,3-dihydroxybiphenyl
-
-
?
2',3'-dichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
25% of activity with 2,3-dihydroxybiphenyl, 57% of activity with 2,3-dihydroxybiphenyl
-
-
?
2',4'-dichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
33% of activity with 2,3-dihydroxybiphenyl, 63% of activity with 2,3-dihydroxybiphenyl
-
-
?
2'-aminobiphenyl-2,3-diol + O2
?
show the reaction diagram
-
-
-
-
?
2'-aminobiphenyl-2,3-diol + O2
(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
show the reaction diagram
Pseudomonas resinovorans, Pseudomonas resinovorans CA10
-
-
-
-
?
2'-chloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
-, 50% of activity with 2,3-dihydroxybiphenyl
-
-
?
2,2',3,3'-tetrachlorobiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
2,2',3-trihydroxybiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
2,2'-dibromobiphenyl + O2
?
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia LB400
-
-
-
-
?
2,2'-dichlorobiphenyl + O2
?
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni B-356
-
-
-
-
?
2,2'-dichlorobiphenyl + O2
cis-5,6-dihydro-5,6-dihydroxy-2,2'-dichlorobiphenyl
show the reaction diagram
-
-
-
-
?
2,2'-difluorobiphenyl + O2
?
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia LB400
-
-
-
-
?
2,2'-dihydroxybiphenyl + O2
6-(2-hydroxyphenyl)-6-oxohexa-2,4-dienoate
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia LB400
-
-
-
-
?
2,2'-dinitrobiphenyl + O2
?
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia LB400
-
-
-
-
?
2,3-dihydroxy-3'-chlorobiphenyl + O2
2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-, Q8GR45
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
ir
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
A9QT35, -
best substrate
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
A9QT35, -
best substrate
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q8GR45
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Ralstonia sp. SBUG
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Burkholderia sp. LB400
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Comamonas sp. SMN4
-
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
11% of activity with 2,3-dihydroxybiphenyl, 26% of activity with 2,3-dihydroxybiphenyl
-
-
?
3',4'-dichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
49% of activity with 2,3-dihydroxybiphenyl, 59% of activity with 2,3-dihydroxybiphenyl, 61% of activity with 2,3-dihydroxybiphenyl
-
-
?
3',5'-dichloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
15% of activity with 2,3-dihydroxybiphenyl, 49% of activity with 2,3-dihydroxybiphenyl, 61% of activity with 2,3-dihydroxybiphenyl
-
-
?
3'-chloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
-, 64% of activity with 2,3-dihydroxybiphenyl, 71% of activity with 2,3-dihydroxybiphenyl, 95% of activity with 2,3-dihydroxybiphenyl
-
-
?
3,3'-dichlorobiphenyl + O2
?
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni B-356
-
-
-
-
?
3,5-dichlorocatechol + O2
3,5-dichloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Sphingomonas sp., Sphingomonas sp. BN6
-
-
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
-
-
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
-
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
Q53126, -
BphC from strain RHA1 weak relative activity
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
Q53126, -
Etbc from strain RDC1
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
Sphingomonas sp. BN6
-
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
Burkholderia cepacia LB400
-
-
-
-
?
3-chlorocatechol + O2
3-chloro-2-hydroxymuconic semialdehyde + H2O
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
BphC from strain RHA1 weak relative activity
-
-
?
3-chlorocatechol + O2
?
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
3-chlorocatechol + O2
?
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
3-ethylcatechol + O2
2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate
show the reaction diagram
Burkholderia cepacia, Burkholderia cepacia LB400
-
-
-
-
?
3-formylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
3-isoporopylcatechol + O2
2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ?
show the reaction diagram
-
relative activity 179%
-
-
?
3-isopropylcatechol + O2
2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ?
show the reaction diagram
-
-
-
-
?
3-methoxycatechol + O2
2-hydroxy-3-methoxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
relative activity 13%
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
cleaved less efficiently, weaker substrate inhibition
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Q53126, -
strain RHA1
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
strain DJ-12
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
strain OU83
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
relative activity 93%
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
strain DJ77
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas putida PpG7
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Sphingomonas sp. BN6
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Ralstonia sp. SBUG
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas sp. DJ77
-
strain DJ77
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Burkholderia cepacia LB400
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
strain RHA1
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Rhodococcus sp. RHA1
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
strain DJ-12
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas pseudoalcaligenes KF714
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas putida KT2442
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas putida KF715
-
strain OU83
-
-
?
3-methylcatechol + O2
2-hydroxy-6-oxohepta-2,4-dienoate
show the reaction diagram
Pseudomonas putida KF715
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-, Q8GR45
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
F2X5L9
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
Q8GR45
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
3-methylcatechol + O2
?
show the reaction diagram
Comamonas sp. SMN4
-
-
-
-
?
4'-chloro-2,3-dihydroxybiphenyl + O2
2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O
show the reaction diagram
-
-, 61% of activity with 2,3-dihydroxybiphenyl, 77% of activity with 2,3-dihydroxybiphenyl, 84% of activity with 2,3-dihydroxybiphenyl
-
-
?
4,4'-dichlorobiphenyl + O2
?
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni B-356
-
-
-
-
?
4-chloro-2,3-dihydroxybiphenyl + O2
3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chloro-2,3-dihydroxybiphenyl + O2
3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chloro-2,3-dihydroxybiphenyl + O2
3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Q52032
-
-
-
?
4-chloro-2,3-dihydroxybiphenyl + O2
3-chloro-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
4-chlorobiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
?
show the reaction diagram
-, Q8GR45
-
-
-
?
4-chlorocatechol + O2
?
show the reaction diagram
Q8GR45
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-, Q52032
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Q52032
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain KKS102
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain DJ77
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas sp. DJ77
-
strain DJ77
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain KKS102
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas putida OU83
Q52032
-
-
-
?
4-chlorocatechol + O2
4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-, Q52032
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Q52032
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain KKS102
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
cleaved less efficiently, weaker substrate inhibition
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Q53126, -
strain RHA1
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain DJ77
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
A9QT35, -
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Ralstonia sp. SBUG
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas sp. DJ77
-
strain DJ77
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
strain RHA1
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
strain KKS102
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas putida OU83
Q52032
-
-
-
?
4-methylcatechol + O2
2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-, Q8GR45
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
Q8GR45
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
4-methylcatechol + O2
?
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
biphenyl + O2
?
show the reaction diagram
Comamonas testosteroni, Comamonas testosteroni B-356
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q53126, -
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-, Q52032
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q52032
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
ring metacleavage
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
ring metacleavage
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
relative activity 100%
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q52032
third step in biphenyl degradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic catabolism of virtually all aromatic growth substrates
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
enzyme activity is constitutive
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida PpG7
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Sphingomonas sp. BN6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
ring metacleavage, aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas sp. DJ77
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Comamonas testosteroni B-356
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Burkholderia cepacia LB400
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Cupriavidus necator H850
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus sp. RHA1
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas pseudoalcaligenes KF714
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida KT2442
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-, ring metacleavage
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida OU83
Q52032
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida KF715
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas sp. CB406
-
-, aerobic catabolism of virtually all aromatic growth substrates
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus erythropolis TA421
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, Q8GR45
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
F2X5L9
-, preferred substrate
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
highly preferred substrate
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Q8GR45
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
highly preferred substrate
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
-
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
-
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
-
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-, Q52032
-
-
-
?
catechol + O2
?
show the reaction diagram
Q52032
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-, Q8GR45
-
-
-
?
catechol + O2
?
show the reaction diagram
A9QT35, -
-
-
-
?
catechol + O2
?
show the reaction diagram
-
strain KKS102
-
-
-
catechol + O2
?
show the reaction diagram
-
cleaved less efficiently, weaker substrate inhibition
-
-
?
catechol + O2
?
show the reaction diagram
Q53126, -
strain RHA1
-
-
?
catechol + O2
?
show the reaction diagram
-
relative activity 7%
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
?
catechol + O2
?
show the reaction diagram
A9QT35, -
-
-
-
?
catechol + O2
?
show the reaction diagram
Q8GR45
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas putida PpG7
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Ralstonia sp. SBUG
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas sp. DJ77
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Burkholderia cepacia LB400
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
strain RHA1
-
-
?
catechol + O2
?
show the reaction diagram
Rhodococcus sp. RHA1
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
strain KKS102
-
-
-
catechol + O2
?
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas pseudoalcaligenes KF714
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas putida KT2442
-
-
-
-
?
catechol + O2
?
show the reaction diagram
-
-
-
-
-
catechol + O2
?
show the reaction diagram
Pseudomonas putida OU83
Q52032
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
-
catechol + O2
?
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
catechol + O2
?
show the reaction diagram
Pseudomonas putida KF715
-
-
-
-
-
catechol + O2
?
show the reaction diagram
Pseudomonas putida KF715
-
-
-
-
?
catechol + O2
2-hydroxymuconate semialdehyde
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
catechol + O2
2-hydroxymuconate semialdehyde
show the reaction diagram
Q6QF56, Q6QF57
-
-
-
?
catechol + O2
2-hydroxymuconic semialdehyde
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
catechol + O2
2-hydroxymuconic semialdehyde
show the reaction diagram
-
-
-
-
?
catechol + O2
2-hydroxymuconic semialdehyde
show the reaction diagram
F2X5L9
-
-
-
?
catechol + O2
2-hydroxymuconic semialdehyde
show the reaction diagram
-
-
-
-
?
catechol + O2
2-hydroxymuconic semialdehyde
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
cis-5,6-dihydro-5,6-dihydroxy-2,2'-dichlorobiphenyl + O2
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
4-methylcatechol, protocatechuic acid, 3,4-dihydroxyphenylacetic acid, gentisic acid and homogentisic acid are no substrates
-
-
-
additional information
?
-
-
3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
-
3-chlorocatechol is no substrate
-
-
-
additional information
?
-
-
strain 3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
Q52032
strain 3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
-
strain CB406, 3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
-
no substrate: biphenyl-3,4-diol, catechol, 3-methylcatechol, 4-methylcatechol
-
-
-
additional information
?
-
-
no activity for protocatechuate
-
-
-
additional information
?
-
-
no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol
-
-
-
additional information
?
-
F2X5L9
activity of recombinant enzyme toward substituted catechols is in descending order: 2,3-dihydroxybiphenyl, 3-methylcatechol, catechol, and 4-chlorocatechol/4-methylcatechol, substrate binding analysis by molecular docking
-
-
-
additional information
?
-
Pseudomonas putida PpG7
-
3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
Ralstonia sp. SBUG
-
no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol
-
-
-
additional information
?
-
Pseudomonas putida KF715
-
3,4-dihydroxybiphenyl is no substrate
-
-
-
additional information
?
-
Pseudomonas sp. CB406
-
strain CB406, 3,4-dihydroxybiphenyl is no substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q53126, -
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
relative activity 100%
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Q52032
third step in biphenyl degradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
aerobic catabolism of virtually all aromatic growth substrates
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, Q8GR45
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
F2X5L9
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
Q8GR45
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida PpG7
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Sphingomonas sp. BN6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
show the reaction diagram
-, B3VTK1, B3VTL5
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
aerobic biphenyl biodegradation pathway
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus globerulus P6
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas sp. DJ77
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Comamonas testosteroni B-356
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Burkholderia cepacia LB400
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Cupriavidus necator H850
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus sp. RHA1
Q53126
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus sp. RHA1
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Sphingobium yanoikuyae Q1
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas pseudoalcaligenes KF714
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida KT2442
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida OU83
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas putida KF715
-
-
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Pseudomonas sp. CB406
-
aerobic catabolism of virtually all aromatic growth substrates
-
-
?
biphenyl-2,3-diol + O2
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
show the reaction diagram
Rhodococcus erythropolis TA421
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
A9QT35, -
-
Ca2+
F2X5L9
1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol
Cd2+
-, Q8GR45
activates
Co2+
Q6QF56, Q6QF57
0.1 mM, stimulates by 154%
Co2+
-, Q8GR45
activates
Cu2+
-, Q8GR45
activates
Fe
Q6QF56, Q6QF57
0.8 mol iron per mol subunit of BphC1; 1.0 mol iron per mol subunit of BphC1
Fe2+
-
significantly enhances activity; significantly enhances activity
Fe2+
Q52032
significantly enhances activity
Fe2+
-
cofactor in the catalytic center
Fe2+
-
required, after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine
Fe2+
-
Fe2+-dependent enzyme
Fe2+
A9QT35, -
is an iron (II)-dependent type I extradiol dioxygenase
Fe2+
-, B3VTK1, B3VTL5
activates 270%
Fe3+
F2X5L9
1 mM, activates
Iron
-
ferrous enzyme
K+
-, Q8GR45
activates
Manganese
Q8GR45
enzyme contains 4.0-4.8 manganese atoms per enzyme molecule, depending on the batch
Mg2+
A9QT35, -
-
Mg2+
-, Q8GR45
activates
Mn2+
Q6QF56, Q6QF57
0.1 mM, stimulates by 150%; 0.1 mM, stimulates by 164%
Ni2+
-, Q8GR45
activates
Zn2+
-, Q8GR45
activates
Mn2+
-
dependent on
additional information
A9QT35, -
Ba2+ has no effect
additional information
-, B3VTK1, B3VTL5
no effect by Fe3+, Mg2+ and Ca2+; no effect by Fe3+, Mg2+ and Ca2+, poor activating effect by Fe2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
Q52032
5 mM 30 min
1,10-phenanthroline
-
-
2,3-Dihydroxybiphenyl
-
-
2,3-Dihydroxybiphenyl
-
enzyme ist strongly inhibited by substrate, Kss 0.0003 M
2,3-Dihydroxybiphenyl
-
reversible substrate inhibition
2,3-Dihydroxybiphenyl
-
-
2-Hydroxypyridine N-oxide
-
0.2 mM
3,4-dihydroxybiphenyl
-
strain CB406, inactivation in presence of O2
3-chlorocatechol
-
competitive inhibition
3-chlorocatechol
-
-
3-chlorocatechol
-
1 mM, strong inhibition
3-chlorocatechol
-
a suicide inhibitor of BphC that oxidizes the active site Fe(II)
3-Ethylcatechol
-
reversible substrate inhibition
3-Methylcatechol
-
-
4-Chlorocatechol
-
-
4-Methylcatechol
-
-
8-hydroxyquinoline
Q52032
5 mM 30 min
acetone
-
competitive inhibition
alpha,alpha'-dipyridyl
-
inhibits catalysis, but protects the Fe2+ from oxidative inactivation
Ba2+
F2X5L9
1 mM, moderate inhibition
Ca2+
-, Q8GR45
-
Ca2+
F2X5L9
1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol
Ca2+
-, B3VTK1, B3VTL5
-
Co2+
Q6QF56, Q6QF57
1 mM, 89% inhibition; complete inhibition by 0.1 mM and 1 mM
Co2+
F2X5L9
1 mM, complete inhibition
Co2+
-, B3VTK1, B3VTL5
-
Cr3+
-, Q8GR45
-
Cu2+
Q6QF56, Q6QF57
0.1 mM caused 92% inhibition; 0.1 mM caused 92% inhibition
Cu2+
F2X5L9
1 mM, complete inhibition
Cu2+
-, B3VTK1, B3VTL5
-
diethyldithiocarbamic acid
Q52032
5 mM 30 min
Fe2+
-, Q8GR45
-
-
Fe2+
-, B3VTK1, B3VTL5
-
-
Fe3+
Q52032
-
-
Fe3+
-
complete inactivation
-
Fe3+
Q6QF56, Q6QF57
0.1 mM, 12% inhibition. 1 mM, 88% inhibition; 0.1 mM, 21% inhibition. 1 mM, 91% inhibition
-
Fe3+
-, B3VTK1, B3VTL5
slight inhibition
-
H2O2
Q6QF56, Q6QF57
0.05 mM, complete inhibition; 0.05 mM, complete inhibition
H2O2
-, Q8GR45
81% inhibition at 10 mM
Hg2+
F2X5L9
1 mM, complete inhibition
iodoacetic acid
Q52032
5 mM 30 min
isopropylalcohol
-
-
KI
A9QT35, -
600 mM reduces specific activity by 35%
KI
-, Q8GR45
43% inhibition at 600 mM
L-ascorbic acid
A9QT35, -
completely inhibits at concentrations over 25 mM
Li+
-, Q8GR45
-
Mg2+
F2X5L9
1 mM, moderate inhibition
Mg2+
-, B3VTK1, B3VTL5
-
Mn2+
Q6QF56, Q6QF57
1 mM, 87% inhibition
N-ethylmaleimide
Q52032
5 mM 30 min
Ni2+
F2X5L9
1 mM, strong inhibition
o-phenanthroline
-
-
SDS
A9QT35, -
1.25 mM inhibits nearly 60%
SDS
-, Q8GR45
46% inhibition at 25 mM
Zn2+
F2X5L9
1 mM, complete inhibition with substrate biphenyl-2,3-diol, not with catechols
Mn2+
F2X5L9
1 mM, moderate inhibition
additional information
-
do not cleave 3,4-dihydroxybiphenyl, but is not inactivated
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.014
-
2'-aminobiphenyl-2,3-diol
-
pH 7.5, 25C
0.001
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.018
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.019
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.0026
-
2,2'-dichlorobiphenyl
-
-
0.000095
-
2,3-Dihydroxybiphenyl
Q8GR45
60C, pH 7.5
0.0003
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
60C
0.001
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
0.001
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
25C
0.003
-
2,3-Dihydroxybiphenyl
-
in presence of 3-chlorocatechol
0.007
-
2,3-Dihydroxybiphenyl
-
-
0.0125
-
2,3-Dihydroxybiphenyl
-
strain OU83
0.014
-
2,3-Dihydroxybiphenyl
-
strain OU83
0.0145
-
2,3-Dihydroxybiphenyl
Q52032
strain OU83
0.0189
-
2,3-Dihydroxybiphenyl
A9QT35, -
pH 8.0, 20C
0.022
-
2,3-Dihydroxybiphenyl
-
-
0.026
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
0.029
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
0.032
-
2,3-Dihydroxybiphenyl
-
Glu79His mutant
0.06
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
25C
0.74
-
2,3-Dihydroxybiphenyl
-
-
0.00045
-
3'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.01
-
3'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.0033
-
3,3'-dichlorobiphenyl
-
-
0.007
-
3,5-dichlorocatechol
-
Glu79His mutant
0.03
-
3,5-dichlorocatechol
-
-
0.025
-
3-chlorocatechol
-
Glu79His mutant
0.1
-
3-chlorocatechol
-
-
0.8
-
3-chlorocatechol
Q6QF56, Q6QF57
60C
1.6
-
3-chlorocatechol
Q6QF56, Q6QF57
25C
2.1
-
3-chlorocatechol
Q6QF56, Q6QF57
25C
0.0108
-
3-formylcatechol
-
in 50 mM Hepes (pH 7.5) at 25C
0.001
-
3-Methylcatechol
Q8GR45
60C, pH 7.5
0.0021
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.0616
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.0688
-
3-Methylcatechol
A9QT35, -
pH 8.0, 20C
0.097
-
3-Methylcatechol
-
Glu79His mutant
0.1
-
3-Methylcatechol
-
25C, pH 7.5
0.1306
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.18
-
3-Methylcatechol
-
-
0.19
-
3-Methylcatechol
-
-
0.49
-
3-Methylcatechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
1.1
-
3-Methylcatechol
Q6QF56, Q6QF57
60C
1.23
-
3-Methylcatechol
-
25C, pH 7.5
1.94
-
3-Methylcatechol
-
25C, pH 7.5
2.1
-
3-Methylcatechol
Q6QF56, Q6QF57
25C
14.6
-
3-Methylcatechol
Q6QF56, Q6QF57
25C
0.0015
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.011
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.012
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
0.025
-
4-Chlorocatechol
Q8GR45
60C, pH 7.5
0.0496
-
4-Chlorocatechol
A9QT35, -
pH 8.0, 20C
0.0273
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.11
-
4-Methylcatechol
Q8GR45
60C, pH 7.5
0.2824
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.3597
-
4-Methylcatechol
A9QT35, -
pH 8.0, 20C
0.3605
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
2.8
-
4-Methylcatechol
-
25C, pH 7.5
5.6
-
4-Methylcatechol
Q6QF56, Q6QF57
25C
7.6
-
4-Methylcatechol
Q6QF56, Q6QF57
60C
11
-
4-Methylcatechol
Q6QF56, Q6QF57
25C
61
-
4-Methylcatechol
-
25C, pH 7.5
93
-
4-Methylcatechol
-
25C, pH 7.5
0.0062
-
Biphenyl
-
-
-
0.00046
-
biphenyl-2,3-diol
-
in 50 mM Hepes (pH 7.5) at 25C
0.00213
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.0049
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.0067
-
biphenyl-2,3-diol
F2X5L9
pH 9.0, 40C, recombinant enzyme
0.0391
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.087
-
biphenyl-2,3-diol
-
pH 9.0, 25C
0.0062
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.01672
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.0589
-
catechol
-
in 50 mM Hepes (pH 7.5) at 25C
0.0976
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
0.1
-
catechol
Q8GR45
60C, pH 7.5
0.12
-
catechol
Q6QF56, Q6QF57
60C
0.281
-
catechol
A9QT35, -
pH 8.0, 20C
0.4
-
catechol
Q6QF56, Q6QF57
25C
0.54
-
catechol
-
-
0.54
-
catechol
Q6QF56, Q6QF57
25C
0.606
-
catechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
0.67
-
catechol
-
Glu79His mutant
1.13
-
catechol
-
-
2.4
-
catechol
-
25C, pH 7.5
0.028
-
O2
-
biphenyl as substrate
0.19
-
O2
-
3,3'-dichlorobiphenyl as substrate
0.75
-
O2
-
2,2'-dichlorobiphenyl as substrate
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.25
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
3.5
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
7.5
-
2'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
4.8
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
9.8
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
12
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
25C
16.5
-
2,3-Dihydroxybiphenyl
-
25C, pH 7.5
17
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
25C
40
-
2,3-Dihydroxybiphenyl
Q6QF56, Q6QF57
60C
84.1
-
2,3-Dihydroxybiphenyl
A9QT35, -
pH 8.0, 20C
4
-
3'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
8.5
-
3'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
12
-
3'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
4
-
3-chlorocatechol
Q6QF56, Q6QF57
25C
9
-
3-chlorocatechol
Q6QF56, Q6QF57
25C
15
-
3-chlorocatechol
Q6QF56, Q6QF57
60C
0.8
-
3-formylcatechol
-
in 50 mM Hepes (pH 7.5) at 25C
3
-
3-Methylcatechol
Q6QF56, Q6QF57
25C
4.4
-
3-Methylcatechol
-
25C, pH 7.5
8
-
3-Methylcatechol
Q6QF56, Q6QF57
25C
9.8
-
3-Methylcatechol
-
25C, pH 7.5
10.15
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
12.02
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
13.6
-
3-Methylcatechol
-
25C, pH 7.5
14
-
3-Methylcatechol
Q6QF56, Q6QF57
60C
16
-
3-Methylcatechol
A9QT35, -
pH 8.0, 20C
21.63
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
44
-
3-Methylcatechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
4.5
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
8.3
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
18.8
-
4'-chloro-2,3-dihydroxybiphenyl
-
25C, pH 7.5
1
-
4-Chlorocatechol
A9QT35, -
pH 8.0, 20C
0.064
-
4-Methylcatechol
-
25C, pH 7.5
0.24
-
4-Methylcatechol
-
25C, pH 7.5
0.36
-
4-Methylcatechol
-
25C, pH 7.5
0.7
-
4-Methylcatechol
A9QT35, -
pH 8.0, 20C
1
-
4-Methylcatechol
Q6QF56, Q6QF57
25C; 60C
1.19
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
2
-
4-Methylcatechol
Q6QF56, Q6QF57
25C
3.25
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
43.2
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8.78
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
53.83
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
86.32
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
115
-
biphenyl-2,3-diol
-
in 50 mM Hepes (pH 7.5) at 25C
165
-
biphenyl-2,3-diol
F2X5L9
pH 9.0, 40C, recombinant enzyme
0.016
-
catechol
-
25C, pH 7.5
0.92
-
catechol
-
25C, pH 7.5
6.7
-
catechol
A9QT35, -
pH 8.0, 20C
7.36
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
9.87
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
15
-
catechol
Q6QF56, Q6QF57
25C
24
-
catechol
Q6QF56, Q6QF57
25C
32.5
-
catechol
-
in 50 mM Hepes (pH 7.5) at 25C
38
-
catechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
50
-
catechol
Q6QF56, Q6QF57
60C
52.56
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
250
-
2'-aminobiphenyl-2,3-diol
-
pH 7.5, 25C
242911
4450
-
2,3-Dihydroxybiphenyl
A9QT35, -
pH 8.0, 20C
1790
89.8
-
3-Methylcatechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
3927
92
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
3927
164.8
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
3927
233
-
3-Methylcatechol
A9QT35, -
pH 8.0, 20C
3927
10300
-
3-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
3927
20
-
4-Chlorocatechol
A9QT35, -
pH 8.0, 20C
4432
2
-
4-Methylcatechol
A9QT35, -
pH 8.0, 20C
4659
4.21
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
4659
9
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
4659
1582
-
4-Methylcatechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
4659
224.6
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8031
10990
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8031
24630
-
biphenyl-2,3-diol
F2X5L9
pH 9.0, 40C, recombinant enzyme
8031
40530
-
biphenyl-2,3-diol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8031
24
-
catechol
A9QT35, -
pH 8.0, 20C
8337
62.7
-
catechol
F2X5L9
pH 9.0, 40C, recombinant enzyme
8337
101.1
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8337
440.2
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8337
8477
-
catechol
-, B3VTK1, B3VTL5
pH 7.5, 30C
8337
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3
-
2,3-Dihydroxybiphenyl
-
reversible substrate inhibition
0.22
-
3-chlorocatechol
-
-
11
-
3-Ethylcatechol
-
-
10.5
-
isopropylalcohol
-
-
5.2
-
tert-butanol
-
catechol as substrate
7.3
-
tert-butanol
-
2,3-dihydroxybiphenyl as substrate
9.9
-
tert-butanol
-
3-methylcatechol as substrate
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.17
-
-
using 4-methylcatechol as substrate
0.33
-
-
using 1,2-dihydroxynaphthalene as substrate; using catechol as substrate
0.52
-
-
cell extract
1.88
-
-
using 1,2-dihydroxydibenzofuran as substrate
2.19
-
-
using 3-isopropylcatechol as substrate
3.1
-
-
BphC1
3.75
-
-
using 3-methylcatechol as substrate
4.79
-
-
using 2,3-dihydroxybiphenyl as substrate
5.47
-
Q8GR45
-
6.88
-
-
after 13.2fold purification
22.2
-
-
-
57.3
-
-
strain OU83
87.2
-
-
pH 9.0, 25C
118.3
-
A9QT35, -
with 2,3-dihydroxybiphenyl as substrate
additional information
-
-
no substrate specificity for 3,4-dihydroxybiphenyl, 2,3-dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
Q8GR45
-
8
9
-, B3VTK1, B3VTL5
-
8
-
Q52032
-
8
-
A9QT35, -
-
8
-
-
optimum pH 8.0 at 30C
8
-
-
assay at
8
-
-, B3VTK1, B3VTL5
-
8.5
-
-, Q8GR45
recombinant enzyme
9
-
Q6QF56, Q6QF57
;
9
-
F2X5L9
recombinant enzyme
9
-
-, B3VTK1, B3VTL5
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
10
-, B3VTK1, B3VTL5
activity range, profile overview; activity range, profile overview; activity range, profile overview
7
9
Q52032
-
7
9
A9QT35, -
pH 7.0: about 50% of maximal activity, pH 9.0: about 45% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
25
-
for meta-cleavage of 1,2-dihydroxydibenzofuran
20
-
-
assay at
25
-
Q52032
-
30
-
-
for meta-cleavage of 2,3-dihydroxybiphenyl
30
-
Q6QF56, Q6QF57
-
30
-
-, B3VTK1, B3VTL5
;
40
-
A9QT35, -
-
40
-
-
optimum temperature is 40C at pH 8.0
40
-
F2X5L9
recombinant enzyme
40
-
-, B3VTK1, B3VTL5
-
50
-
-, Q8GR45
recombinant enzyme
80
-
Q6QF56, Q6QF57
-
85
-
Q8GR45
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
60
-, B3VTK1, B3VTL5
activity range, profile overview; activity range, profile overview; activity range, profile overview
20
70
A9QT35, -
20C: about 75% of maximal activity, 70C: about 85% of maximal activity
20
80
-, Q8GR45
activity range
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
-
isoelectric focusing
PDB
SCOP
CATH
ORGANISM
Burkholderia xenovorans (strain LB400)
Burkholderia xenovorans (strain LB400)
Burkholderia xenovorans (strain LB400)
Burkholderia xenovorans (strain LB400)
Burkholderia xenovorans (strain LB400)
Burkholderia xenovorans (strain LB400)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
Pseudomonas sp. (strain KKS102)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34000
-
Q52032
SDS-PAGE
78000
-
-
heterotetramer, gel-filtration chromatography
125000
-
Q8GR45
gel filtration
190000
-
Q6QF56, Q6QF57
gel filtration
220000
-
Q6QF56, Q6QF57
gel filtration
256000
-
-
gel filtration
265000
-
-
electron microscopy
270000
-
Q52032
gel filtration
300000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 28000, SDS-PAGE; x * 29000, SDS-PAGE; x * 34000, SDS-PAGE
?
-
x * 32000, SDS-PAGE
?
-
x * 32000, SDS-PAGE
?
-, Q8GR45
x * 36000, recombinant enzyme, SDS-PAGE
?
F2X5L9
x * 32000, recombinant enzyme, SDS-PAGE
?
-
x * 36000, recombinant enzyme, SDS-PAGE
-
?
Comamonas sp. SMN4
-
x * 32000, SDS-PAGE
-
?
Rhodococcus globerulus P6
-
x * 28000, SDS-PAGE; x * 29000, SDS-PAGE; x * 34000, SDS-PAGE
-
heterotetramer
-
alpha2beta2 heterotetramer
heterotetramer
-
alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE
heterotetramer
Pseudomonas resinovorans CA10
-
alpha2beta2, 2 * 10000 CarBa, 2 * 29000 CarBb, determination by SDS-PAGE
-
hexamer
-
6 * 21000
hexamer
Q6QF56, Q6QF57
6 * 33000, SDS-PAGE; 6 * 35000, SDS-PAGE
hexamer
Rhodococcus globerulus P6
-
6 * 21000
-
hexamer
-
6 * 33000, SDS-PAGE; 6 * 35000, SDS-PAGE
-
homotetramer
-
4 * 36300, X-ray crystallography
octamer
-
8 * 33200, SDS-PAGE
octamer
-
8 * 31000, SDS-PAGE; 8 * 33000, nucleotide sequence
octamer
-
8 * 32000, strain KKS102, nucleotide sequence
octamer
-
8 * 34000, SDS-PAGE
octamer
-
8 * 31900, nucleotide sequence; 8 * 32000, SDS-PAGE
octamer
Q52032
8 * 34000, SDS-PAGE
octamer
-
8 * 31000, SDS-PAGE
octamer
-
; 8 * 31000, SDS-PAGE; 8 * 33000, nucleotide sequence
-
octamer
Pseudomonas putida KF715
-
8 * 31900, nucleotide sequence; 8 * 32000, SDS-PAGE
-
octamer
Pseudomonas putida OU83
-
-
-
octamer
Pseudomonas putida PpG7
-
8 * 31900, nucleotide sequence; 8 * 32000, SDS-PAGE
-
octamer
-
8 * 32000, strain KKS102, nucleotide sequence
-
octamer
Rhodococcus sp. RHA1
-
8 * 34000, SDS-PAGE
-
tetramer
Q8GR45
4 * 35000, SDS-PAGE
tetramer
-
4 * 35000, SDS-PAGE
-
homotetramer
-
4 * 36300, X-ray crystallography
-
additional information
-
N-terminal amino acid sequence
additional information
F2X5L9
enzyme homology modeling, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting drop vapor diffusion method, using 30% (v/v) PEG 400, 200 mM magnesium chloride, 100 mM Tris-HCl pH 8.5 or 10% (v/v) 2-propanol, 100 mM MES-NaOH pH 6.5 and 200 mM zinc acetate
-
crystals grown from ammoinum sulfate and polyethylene glycol 6000, space groups of P42212 and I222
-
crystals diffract to 1.9 A reslution
-
space group R3, a = b = 136.35 A, c = 106.07 A
-
strain KKS102
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.5
-
-
stable at
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
no loss of activity after 24 h
37
65
Q52032
loses 47% of the activity at 37C and all activity at 65C
50
-
-
30 min full activity
60
-
Q8GR45
no loss of activity after 1 h
60
-
Q6QF56, Q6QF57
1 h, 38% loss of activity; 5 min, complete inactivation
60
-
-
30 min, enzyme retains 30% activity
60
-
-, Q8GR45
purified recombinant enzyme, 60 min, 33% activity remaining
65
-
-
enzyme loses catalytic activity when heated for 10 min
70
-
Q8GR45
25% loss of activity after 1 h
70
-
Q6QF56, Q6QF57
1 h, 59% inhibition
70
-
-
30 min, enzyme completely inactivated
80
-
Q8GR45
approx. 60% loss of activity after 30 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
tert-butanol, glycerol, isopropylalcohol and ethanol stabilizes the enzyme
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
non-heme Fe2+ enzyme which is readily inactivated, oxidizing agents including O2 and H2O2 convert the iron to Fe3+, enzyme can be reactivated by anaerobic incubation in presence of Fe2+ and reducing agents such as thiols or borohydrides
-
439411
shows 82% inactivation after 15 h at room temperature in Tris buffer (50 mM, pH 7.5)
-
674226
50% loss of activity after 4.5 min in air-satured buffer
-
659021
50% loss of activity after 43 min in air-satured buffer
-
659021
50% loss of activity after 7.2 min in air-satured buffer
-
659021
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
stable after addition of 10% acetone or 10% ethanol
-
25C, 30% activity lost when stored for 8 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DEAE Toyopearl column chromatography, phenyl Sepharose column chromatography, and Mono Q column chromatography
-
DEAE-Toyopearl, Phenyl-Sepharose, Mono Q
Q8GR45
anion-exchange, gel filtration
-
by gel filtration, more than 95% pure
A9QT35, -
recombinant enzyme, expressed in Escherichia coli SG13009(pREP4)(pAKC1)
Q52032
Sephadex G-25 column gel filtration
-
nickel nitrilotriacetate resin column chromatography
-
Mono Q HR column chromatography and HiLoad 16/60 Superdex 75 gel filtration
-
recombinant enzyme, expressed in Escherichia coli
-
;
Q6QF56, Q6QF57
strain RDC1, bphC gene mutant
-
recombinant enzyme from Escherichia coli
F2X5L9
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene bphC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21; gene bphC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21; gene bphC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21
-, B3VTK1, B3VTL5
expressed in Escherichia coli MV1190 cells
-
expression in Escherichia coli
Q8GR45
gene BsbphCI, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, the transgenic Escherichia coli strain expressing the BsbphCI gene degrades biphenyl-2,3-diol more quickly than the wild-type strain
-, Q8GR45
bhpC gene hyperexpressed in strain LB400 with pLEBD4
-
strain LB400
-
expressed in Escherichia coli strains DH11S and C41(DE3)
-
genes specifying for transformation of 4-chlorobiphenyl in to 4-chlorbenzoic acid cloned into Pseudomonas putida KT2440 using cosmid pPSA842
-
expression in Escherichia coli strain BL21 (DE3)
-
gene bphC, expressionin Escherichia coli strain BL21 (DE3)
-
subcloned into the pET28(a) vector, yielding pET-bphC and overexpressed in Escherichia coli BL21 (DE3)
A9QT35, -
expressed in Pseudomonas aeruginosa
-
expression in Escherichia coli
-
gene cluster encoding biphenyl and chlorinated degradation cloned
-
nucleoide sequence of bphC gene determined
-
; strain OU83, gene bphC, strain OU83, 2 different 2,3-DBPDs cloned with plasmid pAW590 and expressed in Escherichia coli JM109
-
construction of a genomic library of Pseudomonas putida strain OU83 in cosmid pCP13, expression of the 2,3-dioxygenase gene in Escherichia coli
-
entire bphABCD genes cloned from chromosomal DNA
-
nucleotide sequence of gene bphC cloned into pQE1
Q52032
strain KF715; strain OU83, gene bphC, strain OU83, 2 different 2,3-DBPDs cloned with plasmid pAW590 and expressed in Escherichia coli JM109
-
CarBaBb gene from Pseudomonas resinovorans strain CA10 expressed by expression vector pUCA503 in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
gene encoding 2,3-DHBD from chromosomal DNA of Pseudomonas sp. strain DJ-12
-
strain CB406, meta-cleavage oxygenase BphC cloned
-
strain DJ77, gene phnQ
-
expressed in Escherichia coli strain BL21-SI
-
expressed in Escherichia coli
-
nucleotide sequence of bhpB and 3 bphC genes determined, 2 enzymes expressed in Escherichia coli K38
-
expressed in Escherichia coli BL21(DE3) cellsand Pseudomonas putida strain EG11
-
etbC gene successfully cloned from RCD1 chromosomal DNA
-
gene bphC for meta-cleavage dioxygenase cloned and sequenced from genomic DNA and expressed in Escherichia coli
-
genes BphC1 and BphC2 cloned and expressed in Escherichia coli JM108 and JM109
-
gene bphC_meta, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
F2X5L9
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene bphC2 is constitutively expressed in strain BP3; gene bphC3 is induced by both biphenyl and toluene; the bphC1 gene is only induced by biphenyl
-, B3VTK1, B3VTL5
gene bphC2 is constitutively expressed in strain BP3; gene bphC3 is induced by both biphenyl and toluene; the bphC1 gene is only induced by biphenyl
-
-
constitutively expressed
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
construction of a multistep conversion system of para-substituted phenols by recombinant phenol hydroxylase and 2,3-dihydroxybiphenyl 1,2-dioxygenase using substrates 4-fluorophenol, 4-chlorophenol, 4-bromophenol, 4-nitrophenol, and 4-cresol, mass spectrometric product analysis, overview
additional information
-
usage of the enzyme for construction of a catechol biosensor, method development involving PlackettBurman design and response surface methodology, overview. Maximal specific activity of BphC_LA-4 is about 0.58 U/mg with catechol as substrate. the BphC_LA-4 enzyme electrode prepared by SiO2 solgel shows good response to all these three catecholic compounds
additional information
-
construction of a multistep conversion system of para-substituted phenols by recombinant phenol hydroxylase and 2,3-dihydroxybiphenyl 1,2-dioxygenase using substrates 4-fluorophenol, 4-chlorophenol, 4-bromophenol, 4-nitrophenol, and 4-cresol, mass spectrometric product analysis, overview; usage of the enzyme for construction of a catechol biosensor, method development involving PlackettBurman design and response surface methodology, overview. Maximal specific activity of BphC_LA-4 is about 0.58 U/mg with catechol as substrate. the BphC_LA-4 enzyme electrode prepared by SiO2 solgel shows good response to all these three catecholic compounds
-
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
after loss of ferrous iron, activity can be restored by incubation with ferrous iron in presence of cysteine
-