Application | Comment | Organism |
---|---|---|
degradation | lignocellulose degradation | Agaricus xanthodermus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
3-ethylbenzthiazolin-6-sulfonic acid cation radical | at 30°C with 25 mM glucose as substrate | Agaricus xanthodermus | |
0.02 | - |
3,5-di-t-butyl-1,2-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.05 | - |
2,6-dichloroindophenol | at 30°C with 25 mM glucose as substrate | Agaricus xanthodermus | |
0.12 | - |
ferricenium hexafluorophosphate | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.12 | - |
2-methylcyclohexa-2,5-diene-1,4-dione | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.18 | - |
2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.28 | - |
tetrachloro-1,4-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.33 | - |
ferricyanide | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.39 | - |
tetrafluoro-1,4-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.42 | - |
dimethylaminomethyl-ferricenium | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
0.78 | - |
D-glucose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
0.95 | - |
CuSO4 | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
1.1 | - |
1,4-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
1.6 | - |
2-chloro-1,4-benzoquinone | at 30°C with 25 mM glucose as susbtrate | Agaricus xanthodermus | |
11 | - |
D-galactose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
12 | - |
D-maltose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
17 | - |
D-arabinose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
17 | - |
gentiobiose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
25 | - |
D-xylose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
100 | - |
D-maltotriose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus | |
120 | - |
beta-lactose | at 30°C with 0.4 mM ferricenium as electron acceptor | Agaricus xanthodermus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Agaricus xanthodermus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65400 | - |
SDS-PAGE, gel filtration, MALDI MS | Agaricus xanthodermus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus xanthodermus | Q3L1D2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of 5% | Agaricus xanthodermus |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chromatography | Agaricus xanthodermus |
Storage Stability | Organism |
---|---|
several month at 4°, pH 6.5 in potassium phosphate buffer | Agaricus xanthodermus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone | - |
Agaricus xanthodermus | 2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone | - |
? | |
beta-lactose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? | |
D-arabinose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? | |
D-galactose + benzoquinone | - |
Agaricus xanthodermus | 2,3-didehydro-D-galactose + hydroquinone | two main reaction products | ? | |
D-galactose + benzoquinone | - |
Agaricus xanthodermus | 2-dehydro-D-galactose + hydroquinone | two main reaction products | ? | |
D-glucose + 2,6-dichloroindophenol | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + 3,5-di-t-butyl-1,2-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + benzoquinone | 3-oxo-D-glucose, 1-alpha-3-dehydro-d-glucopyranose, 1-beta-3-dehydro-d-glucopyranose, 1-alpha-3-dehydro-d-glucopyranose hydrate, 1-beta-3-dehydro-d-glucopyranose hydrate, 2-dehydro-d-glucose, 1-alpha-2,3-bis-dehydro-d-glucopyranose bis-hydrate as minor reaction products | Agaricus xanthodermus | 2,3-didehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + CuSO4 | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + dimethylaminomethyl-ferricenium | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + Fe3+ | - |
Agaricus xanthodermus | 3-dehydro-D-glucose + Fe2+ + H+ | - |
? | |
D-glucose + ferricyanide | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + methyl-1,4-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + tetrachloro-1,4-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-glucose + tetrafluoro-1,4-benzoquinone | - |
Agaricus xanthodermus | ? | - |
? | |
D-maltose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? | |
D-maltotriose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? | |
D-xylose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? | |
gentiobiose + ferricenium hexafluorophosphate | - |
Agaricus xanthodermus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Agaricus xanthodermus |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Agaricus xanthodermus |
pyranose dehydrogenase | - |
Agaricus xanthodermus |
pyranose:acceptor oxidoreductase | - |
Agaricus xanthodermus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Agaricus xanthodermus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
for glucose and 1,4-benzoquinone as substrates | Agaricus xanthodermus |
8 | - |
for glucose and 1,4-benzoquinone as substrates | Agaricus xanthodermus |
8.5 | - |
for glucose and ferricenium hexafluorophosphate as substrates | Agaricus xanthodermus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | depending on the electron acceptor and with D-glucose as the electron donor, has 2 pH optima for some substrates | Agaricus xanthodermus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.7 | 7.7 | in potassium phosphate buffer, half life of 126 days at pH 7.7 | Agaricus xanthodermus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalent type of flavin-polypeptide linkage, no release by treatment with 5% trichloric acid and heating | Agaricus xanthodermus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agaricus xanthodermus | isoelectric focusing, five isoforms | - |
4.2 |