Information on EC 1.1.99.29 - pyranose dehydrogenase (acceptor)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.29
-
RECOMMENDED NAME
GeneOntology No.
pyranose dehydrogenase (acceptor)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a pyranose + acceptor = a pyranos-2-ulose (or a pyranos-3-ulose or a pyranos-2,3-diulose) + reduced acceptor
show the reaction diagram
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-
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a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
pyranose:acceptor oxidoreductase
Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.
CAS REGISTRY NUMBER
COMMENTARY hide
190606-21-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
show the reaction diagram
2-dehydrolactose + acceptor
2,3-didehydrolactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
2-deoxy-D-galactose + acceptor
?
show the reaction diagram
2-deoxy-D-glucose + 1,4-benzoquinone
?
show the reaction diagram
-
49% of the activity with D-glucose
-
-
?
2-deoxy-D-glucose + acceptor
?
show the reaction diagram
2-deoxy-D-glucose + ferricenium ion
?
show the reaction diagram
-
21% of the activity with D-glucose
-
-
?
2-hydroxymethylphenyl-beta-D-glucopyranoside + acceptor
?
show the reaction diagram
salicin, relative activity towards D-glucose (100%): 78%
-
-
?
3-dehydro-D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
-
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-
-
?
3-fluoro-3-deoxy-D-glucose + acceptor
? + reduced acceptor
show the reaction diagram
-
-
-
-
?
4-hydroxyphenyl-beta-D-glucopyranoside + acceptor
?
show the reaction diagram
arbutin
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-
?
6-deoxy-D-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 105%
-
-
?
a pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
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?
a pyranoside + acceptor
a 3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
show the reaction diagram
aldopyranose + 1,4-benzoquinone
2-aldoketose + hydroquinone
show the reaction diagram
-
more active as electron acceptor than 2,6-dichlorophenolindophenol or ferricyanide, less active than 3,5-di-tert-butyl-1,2-benzoquinone, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
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-
?
aldopyranose + 2,6-dichlorophenolindophenol
2-aldoketose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
65.9% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
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-
?
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone
2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone
show the reaction diagram
aldopyranose + ferricyanide
2-aldoketose + ferrocyanide
show the reaction diagram
-
10.4% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
aldopyranose + quinone
2-aldoketose + hydroquinone
show the reaction diagram
-
C-2 specific sugar oxidoreductase, highly nonspecific, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses
-
-
?
alpha,alpha-trehalose + 1,4-benzoquinone
3,3'-didehydro-alpha,alpha-trehalose + hydroquinone
show the reaction diagram
alpha,alpha-trehalose + acceptor
3,3'-didehydro-alpha,alpha-trehalose + reduced acceptor
show the reaction diagram
relative activity towards D-glucose (100%): 27%
-
-
?
beta-lactose + acceptor
?
show the reaction diagram
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-
-
?
beta-lactose + ferricenium hexafluorophosphate
?
show the reaction diagram
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-
-
?
cellobiitol + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 77%
-
-
?
cellobiose + 1,4-benzoquinone
?
show the reaction diagram
cellobiose + 2,6-dichlorophenolindophenol
2-dehydro-cellobiose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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-
-
-
?
cellobiose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 70%
-
-
?
cellobiose + Fe3+
?
show the reaction diagram
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-
-
-
?
cellobiose + ferricenium ion
?
show the reaction diagram
-
7% of the activity with D-glucose
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?
cellobiose + ferricenium ion
? + ferrocene
show the reaction diagram
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-
?
cellobiose + ferrocenium ion
2-dehydro-cellobiose + ferrocene
show the reaction diagram
cellotetraose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 14%
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-
?
cellotriose + 1,4-benzoquinone
?
show the reaction diagram
-
56% of the activity with D-glucose
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?
D-allose + 1,4-benzoquinone
2-dehydro-D-allose + hydroquinone
show the reaction diagram
-
30% of the activity with D-glucose
-
-
?
D-allose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 15%
-
-
?
D-allose + ferricenium ion
? + ferrocene
show the reaction diagram
Q0R4L2, Q3L243, Q3L425
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-
-
?
D-arabino-2-hexosulose + 1,4-benzoquinone
?
show the reaction diagram
-
59% of the activity with D-glucose
-
-
?
D-arabinose + 1,4-benzoquinone
2-dehydro-D-arabinose + hydroquinone
show the reaction diagram
-
18% of the activity with D-glucose
-
-
?
D-arabinose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-cellobiose + acceptor
?
show the reaction diagram
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-
-
-
?
D-fructose + acceptor
?
show the reaction diagram
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-
-
?
D-fructose + Fe3+
?
show the reaction diagram
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-
-
-
?
D-fructose + ferricenium ion
? + ferrocene
show the reaction diagram
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-
-
-
?
D-galactose + 1,4-benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
D-galactose + 1,4-benzoquinone
?
show the reaction diagram
-
66% of the activity with D-glucose
-
-
?
D-galactose + acceptor
2-dehydro-D-galactose + reduced acceptor
show the reaction diagram
relative activity towards D-glucase (100%): 99%
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-
?
D-galactose + acceptor
?
show the reaction diagram
D-galactose + benzoquinone
2,3-didehydro-D-galactose + hydroquinone
show the reaction diagram
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-
two main reaction products
-
?
D-galactose + benzoquinone
2-dehydro-D-galactose + hydroquinone
show the reaction diagram
D-galactose + Fe3+
2-dehydro-D-galactose + Fe2+ + H+
show the reaction diagram
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-
?
D-galactose + ferricenium ion
?
show the reaction diagram
-
60% of the activity with D-glucose
-
-
?
D-galactose + ferricenium ion
? + ferrocene
show the reaction diagram
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
show the reaction diagram
D-glucono-1,5-lactone + 1,4-benzoquinone
?
show the reaction diagram
D-glucono-1,5-lactone + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 42%
-
-
?
D-glucono-1,5-lactone + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
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?
D-glucosamine + acceptor
?
show the reaction diagram
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-
-
-
?
D-glucose
3-dehydro-D-glucose + Fe2+ + H+
show the reaction diagram
-
pH 8.5
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-
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + ?
show the reaction diagram
D-glucose + 1,4-benzoquinone
2-dehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
D-glucose + 2 1,4-benzoquinone
2,3-didehydro-D-glucose + 2 hydroquinone
show the reaction diagram
-
-
-
-
?
D-glucose + 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
2-dehydro-D-glucose + reduced 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
show the reaction diagram
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
2-dehydro-D-glucose + ?
show the reaction diagram
Q0R4L2, Q3L243, Q3L425
-
-
-
?
D-glucose + 2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
3-dehydro-D-glucose + ?
show the reaction diagram
Q0R4L2, Q3L243, Q3L425
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-
-
?
D-glucose + 2,5-chloro-1,4-benzoquinone
?
show the reaction diagram
-
pH 5
-
-
?
D-glucose + 2,6-dichloroindophenol
?
show the reaction diagram
D-glucose + 2,6-dichlorophenolindophenol
2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
D-glucose + 2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
D-glucose + 3,5-di-t-butyl-1,2-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone
?
show the reaction diagram
-
pH 6
-
-
?
D-glucose + 3-ethylbenzthiazolin-6-sulfonic acid cation radical
?
show the reaction diagram
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-
-
-
?
D-glucose + acceptor
2,3-didehydro-D-glucose + reduced acceptor
show the reaction diagram
-
dioxidation at C2 and C3
-
-
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
show the reaction diagram
D-glucose + acceptor
3-dehydro-D-glucose + reduced acceptor
show the reaction diagram
D-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 100%
-
-
?
D-glucose + benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + CuSO4
?
show the reaction diagram
-
-
-
-
?
D-glucose + dimethylaminomethyl-ferricenium
?
show the reaction diagram
-
-
-
-
?
D-glucose + Fe3+
3-dehydro-D-glucose + Fe2+ + H+
show the reaction diagram
-
-
-
-
?
D-glucose + ferricenium ion
2-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferricyanide
?
show the reaction diagram
-
-
-
-
?
D-glucose + ferrocenium ion
2-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
-
?
D-glucose + methyl-1,4-benzoquinone
?
show the reaction diagram
D-glucose + O2
2-dehydro-D-glucose + H2O2
show the reaction diagram
-
-
-
-
?
D-glucose + tetrachloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + tetrafluoro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-gulose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 7%
-
-
?
D-lyxose + 1,4-benzoquinone
?
show the reaction diagram
-
5% of the activity with D-glucose
-
-
?
D-lyxose + ferricenium ion
?
show the reaction diagram
-
5% of the activity with D-glucose
-
-
?
D-maltoheptaose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltopentaose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltose + acceptor
?
show the reaction diagram
D-maltose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-maltotriose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-maltotriose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-maltotriose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-mannose + 1,4-benzoquinone
?
show the reaction diagram
-
32% of the activity with D-glucose
-
-
?
D-mannose + acceptor
2-dehydro-D-mannose + reduced acceptor
show the reaction diagram
-
-
-
-
?
D-mannose + acceptor
?
show the reaction diagram
D-mannose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-mannose + ferricenium ion
?
show the reaction diagram
-
26% of the activity with D-glucose
-
-
?
D-mannose + ferricenium ion
? + ferrocene
show the reaction diagram
D-mannose + ferrocenium ion
2-dehydro-D-mannose + ferrocene
show the reaction diagram
D-ribose + 1,4-benzoquinone
2-dehydro-D-ribose + hydroquinone
show the reaction diagram
-
34% of the activity with D-glucose
-
-
?
D-ribose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 19%
-
-
?
D-ribose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-ribose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-ribulose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 17%
-
-
?
D-sorbose + acceptor
?
show the reaction diagram
-
-
-
?
D-tagatose + acceptor
?
show the reaction diagram
relative activity towards D-glucoase (100%): 67%
-
-
?
D-talose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 10%
-
-
?
D-talose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-talose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-trehalose + acceptor
?
show the reaction diagram
-
-
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
show the reaction diagram
D-xylose + 1,4-benzoquinone
?
show the reaction diagram
-
72% of the activity with D-glucose
-
-
?
D-xylose + 2 1,4-benzoquinone
2,3-didehydro-D-xylose + 2 hydroquinone
show the reaction diagram
-
-
-
?
D-xylose + acceptor
?
show the reaction diagram
D-xylose + Fe3+
?
show the reaction diagram
-
-
-
-
?
D-xylose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
D-xylose + ferricenium ion
?
show the reaction diagram
-
81% of the activity with D-glucose
-
-
?
D-xylose + ferricenium ion
? + ferrocene
show the reaction diagram
D-xylose + ferrocenium ion
2-dehydro-D-xylose + ferrocene
show the reaction diagram
D-xylose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
erlose + 1,4-benzoquinone
3-dehydroerlose + hydroquinone
show the reaction diagram
gentiobiose + ferricenium hexafluorophosphate
?
show the reaction diagram
-
-
-
-
?
L-arabinose + 1,4-benzoquinone
2-dehydro-L-arabinose + hydroquinone
show the reaction diagram
-
113% of the activity with D-glucose
-
-
?
L-arabinose + 1,4-benzoquinone
?
show the reaction diagram
-
54% of the activity with D-glucose
-
-
?
L-arabinose + acceptor
?
show the reaction diagram
L-arabinose + Fe3+
?
show the reaction diagram
-
-
-
-
?
L-arabinose + ferricenium ion
?
show the reaction diagram
-
72% of the activity with D-glucose
-
-
?
L-arabinose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferrocenium ion
2-dehydro-L-arabinose + ferrocene
show the reaction diagram
L-arabinose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-fucose + acceptor
?
show the reaction diagram
L-glucose + 1,4-benzoquinone
2-dehydro-L-glucose + hydroquinone
show the reaction diagram
-
61% of the activity with D-glucose
-
-
?
L-glucose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 42%
-
-
?
L-glucose + ferricenium ion
?
show the reaction diagram
-
11% of the activity with D-glucose
-
-
?
L-sorbose + 1,4-benzoquinone
2-dehydro-L-sorbose + hydroquinone
show the reaction diagram
-
10% of the activity with D-glucose
-
-
?
L-sorbose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 5%
-
-
?
L-sorbose + Fe3+
?
show the reaction diagram
-
-
-
-
?
L-sorbose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-xylobiose + Fe3+
?
show the reaction diagram
-
-
-
-
?
lactose + 1,4-benzoquinone
?
show the reaction diagram
-
21% of the activity with D-glucose
-
-
?
lactose + acceptor
2-dehydrolactose + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 15%
-
-
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
show the reaction diagram
lactose + acceptor
lactobiono-1,5-lactone + reduced acceptor
show the reaction diagram
-
-
-
-
?
lactose + Fe3+
?
show the reaction diagram
-
-
-
-
?
lactose + ferricenium ion
? + ferrocene
show the reaction diagram
lactose + ferrocenium ion
2-dehydro-lactose + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + ferrocenium ion
? + ferrocene
show the reaction diagram
maltose + 1,4-benzoquinone
?
show the reaction diagram
maltose + ferricenium ion
?
show the reaction diagram
-
45% of the activity with D-glucose
-
-
?
maltose + ferricenium ion
? + ferrocene
show the reaction diagram
Q0R4L2, Q3L243, Q3L425
-
-
-
?
maltose + ferrocenium ion
2-dehydro-maltose + ferrocene
show the reaction diagram
-
-
-
-
?
maltotetraose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 9%
-
-
?
maltotriose + 1,4-benzoquinone
?
show the reaction diagram
maltotriose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 12%
-
-
?
maltotriose + ferricenium ion
?
show the reaction diagram
-
14% of the activity with D-glucose
-
-
?
maltotriose + ferricenium ion
? + ferrocene
show the reaction diagram
melezitose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 13%
-
-
?
melibiose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 48%
-
-
?
melizitose + 1,4-benzoquinone
3-dehydromelizitose + hydroquinone
show the reaction diagram
methyl alpha-D-glucopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 107%
-
-
?
methyl alpha-D-mannopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 5%
-
-
?
methyl beta-D-glucopyranoside + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 58%
-
-
?
methyl-alpha-D-galactopyranose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 17%
-
-
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
11% of the activity with D-glucose
-
-
?
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone
show the reaction diagram
methyl-alpha-D-galactopyranoside + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
-
-
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
141% of the activity with D-glucose
-
-
?
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone
methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone
show the reaction diagram
methyl-alpha-D-glucopyranoside + ferricenium ion
?
show the reaction diagram
-
143% of the activity with D-glucose
-
-
?
methyl-alpha-D-glucopyranoside + ferricenium ion
? + ferrocene
show the reaction diagram
Q0R4L2, Q3L243, Q3L425
-
-
-
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
?
show the reaction diagram
-
30% of the activity with D-glucose
-
-
?
methyl-beta-D-glucopyranoside + 1,4-benzoquinone
methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone
show the reaction diagram
methyl-beta-D-glucopyranoside + ferricenium ion
?
show the reaction diagram
-
17% of the activity with D-glucose
-
-
?
palatinose + acceptor
?
show the reaction diagram
relative activity towards D-glucose (100%): 89%
-
-
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
3-dehydropyranose + reduced acceptor
show the reaction diagram
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
raffinose + ferrocenium ion
2-dehydro-raffinose + ferrocene
show the reaction diagram
-
-
-
-
?
sucrose + 1,4-benzoquinone
3'-dehydrosucrose + hydroquinone
show the reaction diagram
-
quinone-dependent PDH oxidizes sucrose exclusively at C-3 of its terminal glucopyranosyl moiety, 11% of the activity with D-glucose
oxidation of sucrose at C-3 of its terminal glucopyranosyl moiety
-
?
sucrose + 1,4-benzoquinone
?
show the reaction diagram
sucrose + acceptor
3'-dehydrosucrose + reduced acceptor
show the reaction diagram
relative activity towards D-glucose (100%): 46%
-
-
?
sucrose + acceptor
?
show the reaction diagram
-
-
-
-
?
sucrose + ferricenium ion
?
show the reaction diagram
-
6% of the activity with D-glucose
-
-
?
trehalose + 1,4-benzoquinone
?
show the reaction diagram
trehalose + ferricenium ion
?
show the reaction diagram
-
8% of the activity with D-glucose
-
-
?
xylobiose + acceptor
?
show the reaction diagram
-
-
-
?
xylobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,3-didehydro-D-glucose + 1,4-benzoquinone
3-dehydro-D-glucose + hydroquinone
show the reaction diagram
2-dehydro-D-xylose + 1,4-benzoquinone
2,3-didehydro-D-xylose + hydroquinone
show the reaction diagram
a pyranoside + acceptor
a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
show the reaction diagram
-
-
-
-
?
a pyranoside + acceptor
a 3-dehydropyranoside + reduced acceptor
show the reaction diagram
cellobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-fructose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-galactose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-galactose + ferrocenium ion
2-dehydro-D-galactose + ferrocene
show the reaction diagram
-
-
-
-
?
D-glucose + 1,4-benzoquinone
2,3-didehydro-D-glucose + hydroquinone
show the reaction diagram
D-glucose + 1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + 2-chloro-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
D-glucose + acceptor
2,3-didehydro-D-glucose + reduced acceptor
show the reaction diagram
-
dioxidation at C2 and C3
-
-
?
D-glucose + acceptor
2-dehydro-D-glucose + reduced acceptor
show the reaction diagram
-
the enzyme oxidizes D-glucose at both the C2 and C3 position but the C2 position is the primary site of attack
-
-
?
D-glucose + ferricenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
D-glucose + ferrocenium ion
3-dehydro-D-glucose + ferrocene
show the reaction diagram
-
-
-
-
?
D-maltose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-mannose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-ribose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-talose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-xylose + 1,4-benzoquinone
2-dehydro-D-xylose + hydroquinone
show the reaction diagram
D-xylose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
D-xylose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-arabinose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
L-sorbose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + acceptor
lactobiono-1,5-lactone + 2-dehydrolactose + 2,3-didehydrolactose + reduced acceptor
show the reaction diagram
lactose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
lactose + ferrocenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
maltotriose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
pyranose + acceptor
2,3-didehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
2-dehydropyranose + reduced acceptor
show the reaction diagram
pyranose + acceptor
3-dehydropyranose + reduced acceptor
show the reaction diagram
pyranoside + acceptor
3,4-didehydropyranoside + reduced acceptor
show the reaction diagram
xylobiose + ferricenium ion
? + ferrocene
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ag+
-
37% inhibition of activity
Al3+
-
no activity
Ca2+
-
no activity
Cu2+
-
no activity
Fe2+
-
no activity
Fe3+
-
39% inhibition of activity
Hg2+
-
25% inhibition of activity
Mg2+
-
no activity
Mn2+
-
no activity
Ni2+
-
no activity
Zn2+
-
no activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
10 mM, 100% inhibition
alpha,alpha-dipyridyl
-
no activity
Cu2+
-
10 mM, 90% inhibition
EDTA
-
no activity
F-
-
no activity
HgCl2
-
10 mM, 100% inhibition
MoO42-
-
no activity
NaN3
-
no activity
NH4+
-
at 2 M in 20 mM sodium phosphate buffer pH 7.0
p-hydroxymercuribenzoate
-
no activity
Phenanthroline
-
no activity
additional information
-
not inhibited by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Mn2+, NH4+, Zn2+, F-, EDTA, NaN3, dithiothreitol, phenanthroline and H2O2, each at 10 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
50 mM: activates, 100 mM: 100% increase in activity at pH 4
NH4+
-
at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0325 - 3.64
1,4-benzoquinone
0.079
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
0.0132 - 0.109
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
0.92
2,5-chloro-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.05
2,6-dichloroindophenol
-
at 30C with 25 mM glucose as substrate
0.02 - 0.14
2,6-dichlorophenolindophenol
0.18
2-(hydroxymethyl)-6-methoxy-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.55 - 1.6
2-chloro-1,4-benzoquinone
12.14
2-deoxy-D-galactose
-
0.1 M ethanolamine buffer at pH 9.4
0.65
2-deoxy-D-glucose
-
0.1 M ethanolamine buffer at pH 9.4
0.12
2-methylcyclohexa-2,5-diene-1,4-dione
-
at 30C with 25 mM glucose as susbtrate
0.02
3,5-di-t-butyl-1,2-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.22
3,5-di-tert-butyl-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.0017
3-ethylbenzthiazolin-6-sulfonic acid cation radical
-
at 30C with 25 mM glucose as substrate
10.84 - 120
beta-lactose
2.45 - 8.13
cellobiose
0.95
CuSO4
-
at 30C with 25 mM glucose as susbtrate
18.3 - 181
D-Allose
17
D-arabinose
-
at 30C with 0.4 mM ferricenium as electron acceptor
1.05
D-cellobiose
-
0.1 M ethanolamine buffer at pH 9.4
153
D-fructose
0.64 - 74.99
D-galactose
0.74
D-glucosamine
-
0.1 M ethanolamine buffer at pH 9.4
0.35 - 3.85
D-glucose
0.51
D-maltoheptaose
-
0.1 M ethanolamine buffer at pH 9.4
0.64
D-maltopentaose
-
0.1 M ethanolamine buffer at pH 9.4
0.34 - 12
D-maltose
0.64 - 100
D-maltotriose
0.62 - 380
D-mannose
59.7
D-ribose
137
D-sorbose
ferricenium ion as electron acceptor; ferricenium ion as electron acceptor; ferricenium ion as electron acceptor
79.1
D-talose
0.05
D-trehalose
-
0.1 M ethanolamine buffer at pH 9.4
10.8
D-xylobiose
-
Fe3+ as electron acceptor
1.03 - 29.2
D-xylose
0.42
dimethylaminomethyl-ferricenium
-
at 30C with 25 mM glucose as susbtrate
0.13
Fe3+
-
at 30C with 50 mM glucose as susbtrate
0.12
ferricenium hexafluorophosphate
-
at 30C with 25 mM glucose as susbtrate
0.0103 - 0.22
ferricenium ion
0.33
ferricyanide
-
at 30C with 25 mM glucose as susbtrate
0.03 - 1.19
ferrocenium ion
17
gentiobiose
-
at 30C with 0.4 mM ferricenium as electron acceptor
0.44 - 24
L-arabinose
12.31
L-fucose
-
0.1 M ethanolamine buffer at pH 9.4
137
L-sorbose
40.51 - 2288
lactose
2.16 - 11.6
maltose
54.6 - 156
maltotriose
0.18
methyl-1,4-benzoquinone
-
at 30C with 50 mM glucose as susbtrate
0.66 - 2
methyl-alpha-D-glucopyranoside
0.095 - 0.5
O2
10.79
raffinose
-
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
0.35
Sucrose
-
0.1 M ethanolamine buffer at pH 9.4
0.28
tetrachloro-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
0.39
tetrafluoro-1,4-benzoquinone
-
at 30C with 25 mM glucose as susbtrate
10.8
xylobiose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.57 - 76
1,4-benzoquinone
101.2
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
4.28 - 7.57
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
2.8 - 57.6
2,6-dichlorophenolindophenol
12.9 - 15.1
2-chloro-1,4-benzoquinone
25.63 - 55.1
cellobiose
18.4 - 40.2
D-Allose
16.5
D-fructose
5.23 - 137
D-galactose
3 - 50.8
D-glucose
43.2
D-maltose
13.31 - 109
D-mannose
31.4
D-ribose
15.7
D-sorbose
ferricenium ion as electron acceptor; ferricenium ion as electron acceptor; ferricenium ion as electron acceptor
21.3
D-talose
6.37 - 146
D-xylose
9.86 - 203
ferricenium ion
19.92 - 130
ferrocenium ion
3.05 - 45.7
L-arabinose
15.7
L-sorbose
-
with ferrocenium ion as cosubstrate, pH and temperature not specified in the publication
3.12 - 209
lactose
0.48 - 44.7
maltose
16.3 - 35.9
maltotriose
1 - 85
methyl-alpha-D-glucopyranoside
19.61
raffinose
-
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30C
50.4
xylobiose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.89 - 780
1,4-benzoquinone
768
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
with D-glucose as cosubstrate, in 100 mM sodium phosphate pH 7.5, at 30C
38.9 - 532
2,2'-azino-bis[3-ethylbenzothiazoline]-6-sulfonic acid
-
85 - 958
2,6-dichlorophenolindophenol
20.9 - 25.5
2-chloro-1,4-benzoquinone