Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2-bipyridine | 1 mM, 41% inhibition | Phanerodontia chrysosporium | |
Sodium azide | 1 mM, 19% inhibition | Phanerodontia chrysosporium | |
Sodium cyanide | 1 mM, 70% inhibition | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
sedimentation equilibrium centrifugation | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + O2 | Phanerodontia chrysosporium | role in cellulose degradation | cellobiono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
white-rot fungus | - |
Phanerodontia chrysosporium | - |
name for perfect stage of this fungus, imperfect stage is named Sporotrichum pulverulentum | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | binds to immobilized concanavalin A | Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
138fold purification | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-beta-glucosylmannose + O2 | 50% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? | |
cellobiose + O2 | 162% of the activity with lactose | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
cellobiose + O2 | enzyme consumes equimolar amounts of oxygen and cellobiose | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
cellobiose + O2 | readily oxidized, cellobiose and cellohexaose with almost same activity | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
cellobiose + O2 | role in cellulose degradation | Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | - |
? | |
cellodextrin + O2 | high activity towards cellodextrins | Phanerodontia chrysosporium | aldonic acid + H2O2 | - |
? | |
cellodextrin + O2 | decreasing rapidity in the series from cellobiose to cellotetraose, but then with increasing size they are more rapidly oxidized | Phanerodontia chrysosporium | aldonic acid + H2O2 | - |
? | |
galactose + O2 | 26% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? | |
lactose + O2 | readily oxidized | Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2 | product is lactobionic acid | ? | |
maltose + O2 | 22% of the activity with lactose | Phanerodontia chrysosporium | 4-O-beta-glucosyl-glucono-1,5-lactone + H2O2 | - |
? | |
additional information | not as substrates: glucose, xylose, cellulose, galactobiose, gentiobiose, H2O2 not detected as product | Phanerodontia chrysosporium | ? | - |
? | |
sophorose + O2 | 17% of the activity with lactose | Phanerodontia chrysosporium | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.1 | 5.7 | pH 4.1: less than 50% of maximum activity, pH 5.7: less than 50% of maximum activity | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavohemoprotein, one flavin per enzyme molecule | Phanerodontia chrysosporium | |
flavin | flavin component probably is the sugar dehydrogenating unit in the enzyme | Phanerodontia chrysosporium | |
heme b | flavohemoprotein | Phanerodontia chrysosporium | |
heme b | heme component serves for the storage of electrons to be incorporated into molecular oxygen for direct reduction to water | Phanerodontia chrysosporium |