Application | Comment | Organism |
---|---|---|
drug development | the enzyme showed significant cytotoxicity on breast (MCF-7), nasopharyngeal (KB) and ovarian (OVCAR) cancer cell lines at very low concentrations ranging from 0.000093 to 0.00014 mM. It exhibits relatively less cytotoxicity on primary mouse embryonic fibroblast (3T3) cells. Thus, cholesterol oxidase from Streptomyces sp. AKHSS could be a potential anticancer agent | Streptomyces sp. AKHSS |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Streptomyces sp. AKHSS |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
cholesterol | pH 9.0, 37°C | Streptomyces sp. AKHSS |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound, not secreted | Streptomyces sp. AKHSS | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgSO4 | stimulates the enzyme activity | Streptomyces sp. AKHSS | |
ZnSO4 | stimulates the enzyme activity | Streptomyces sp. AKHSS |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42840 | - |
electron-spray ionization mass spectrometry | Streptomyces sp. AKHSS |
48000 | - |
SDS-PAGE | Streptomyces sp. AKHSS |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. AKHSS | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polyethylene glycol 4000 precipitation, diethylaminoethyl Sephacel anionic column chromatography and Superdex-200 gel filtration | Streptomyces sp. AKHSS |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces sp. AKHSS | cholest-5-en-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Streptomyces sp. AKHSS |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable up to | Streptomyces sp. AKHSS |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces sp. AKHSS |