Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanosine hydrochloride | the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl | Brugia malayi | |
N-ethylmaleimide | blocking the SH group leads to activation of rBmG6PD activity | Brugia malayi |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain C41 | Brugia malayi |
General Stability | Organism |
---|---|
unfolding equilibrium of rBmG6PD with GdmCl/urea shows the triphasic unfolding pattern along with the highly stable intermediate obtained by GdmCl | Brugia malayi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5' [beta,gamma-amido] triphosphate | - |
Brugia malayi | |
adenosine 5' [beta-thio] diphosphate | - |
Brugia malayi | |
adenosine 5' [gamma-thio] triphosphate | - |
Brugia malayi | |
ADP | 15% inhibition at 2 mM | Brugia malayi | |
AMP | 5% inhibition at 2 mM | Brugia malayi | |
ATP | 31% inhibition at 5 mM, non-competitive inhibition with respect to D-glucose 6-phosphate | Brugia malayi | |
Cu2+ | - |
Brugia malayi | |
guanosine hydrochloride | the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl | Brugia malayi | |
K+ | - |
Brugia malayi | |
Li+ | - |
Brugia malayi | |
Na+ | - |
Brugia malayi | |
NADPH | competitive inhibition with respect to NADP+ and D-glucose 6-phosphate | Brugia malayi | |
phosphoenol pyruvate | 25% inhibition at 10 mM | Brugia malayi | |
suramin | 77% inhibition at 0.05 mM | Brugia malayi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Brugia malayi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Brugia malayi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
4 * 75000, recombinant His-tagged enzyme, SDS-PAGE | Brugia malayi |
300000 | - |
recombinant His-tagged enzyme, gel filtration | Brugia malayi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Brugia malayi | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brugia malayi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain C41 by nickel affinity chromatography | Brugia malayi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Brugia malayi | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | rBmG6PD is composed of 37% alpha-helices and 26% beta-sheets | Brugia malayi |
tetramer | 4 * 75000, recombinant His-tagged enzyme, SDS-PAGE | Brugia malayi |
Synonyms | Comment | Organism |
---|---|---|
G6PD | - |
Brugia malayi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Brugia malayi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant His-tagged enzyme, inactivation | Brugia malayi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Brugia malayi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Brugia malayi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | versus D-glucose 6-phosphate, pH 8.0, 25°C | Brugia malayi | |
0.04 | - |
NADPH | versus NADP+, pH 8.0, 25°C | Brugia malayi | |
6.5 | - |
ATP | pH 8.0, 25°C | Brugia malayi |
General Information | Comment | Organism |
---|---|---|
metabolism | regulatory enzyme of the pentose phosphate pathway | Brugia malayi |