BRENDA - Enzyme Database show
show all sequences of 1.1.1.43

Purification and characterization of cytosolic 6-phosphogluconate dehydrogenase in isozymes from maize

Bailey-Serres, J.; Nguyen, M.T.; Plant Physiol. 100, 1580-1583 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
NADPH
-
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
NADP+
7.5 mM MgCl2
Zea mays
0.0422
-
NADP+
-
Zea mays
0.14
-
6-phospho-D-gluconate
7.5 mM MgCl2
Zea mays
0.15
-
6-phospho-D-gluconate
1 mM MnCl2
Zea mays
0.25
-
NADP+
0.01 mM NADPH
Zea mays
0.32
-
6-phospho-D-gluconate
-
Zea mays
0.39
-
6-phospho-D-gluconate
0.01 mM NADPH
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Zea mays
5737
-
cytosol
-
Ricinus communis
5829
-
cytosol
-
Zea mays
5829
-
plastid
-
Zea mays
9536
-
proplastid
-
Ricinus communis
9537
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Candida boidinii
Mg2+
stimulated by MgCl2
Ricinus communis
Mg2+
activity increases slightly with 7.5 mM MgCl2
Zea mays
Mn2+
activity increases slightly with 7.5 mM MnCl2
Zea mays
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
SDS-PAGE
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-phospho-D-gluconate + NAD(P)+
Spinacia oleracea
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Nicotiana tabacum
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Candida boidinii
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Ricinus communis
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Zea mays
enzyme belongs to the oxidative pentose phosphate pathway
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida boidinii
-
methanol utilizing yeast
-
Nicotiana tabacum
-
tobacco
-
Ricinus communis
-
castor bean
-
Spinacia oleracea
-
spinach
-
Zea mays
-
maize, L. Pioneer HiBred International line B73
-
Purification (Commentary)
Commentary
Organism
-
Ricinus communis
-
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Ricinus communis
-
leaf
-
Spinacia oleracea
-
leaf
-
Zea mays
-
root
from seedlings
Zea mays
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
20.8
-
-
Zea mays
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + NAD(P)+
-
286822
Spinacia oleracea
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Nicotiana tabacum
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Candida boidinii
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Ricinus communis
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
enzyme belongs to the oxidative pentose phosphate pathway
286822
Zea mays
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
-
Zea mays
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8
-
Ricinus communis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADPH
-
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
NADP+
7.5 mM MgCl2
Zea mays
0.0422
-
NADP+
-
Zea mays
0.14
-
6-phospho-D-gluconate
7.5 mM MgCl2
Zea mays
0.15
-
6-phospho-D-gluconate
1 mM MnCl2
Zea mays
0.25
-
NADP+
0.01 mM NADPH
Zea mays
0.32
-
6-phospho-D-gluconate
-
Zea mays
0.39
-
6-phospho-D-gluconate
0.01 mM NADPH
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Zea mays
5737
-
cytosol
-
Ricinus communis
5829
-
cytosol
-
Zea mays
5829
-
plastid
-
Zea mays
9536
-
proplastid
-
Ricinus communis
9537
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Candida boidinii
Mg2+
stimulated by MgCl2
Ricinus communis
Mg2+
activity increases slightly with 7.5 mM MgCl2
Zea mays
Mn2+
activity increases slightly with 7.5 mM MnCl2
Zea mays
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
SDS-PAGE
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-phospho-D-gluconate + NAD(P)+
Spinacia oleracea
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Nicotiana tabacum
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Candida boidinii
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Ricinus communis
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
6-phospho-D-gluconate + NAD(P)+
Zea mays
enzyme belongs to the oxidative pentose phosphate pathway
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ricinus communis
-
Zea mays
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Ricinus communis
-
leaf
-
Spinacia oleracea
-
leaf
-
Zea mays
-
root
from seedlings
Zea mays
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
20.8
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6-phospho-D-gluconate + NAD(P)+
-
286822
Spinacia oleracea
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Nicotiana tabacum
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Candida boidinii
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
-
286822
Ricinus communis
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
6-phospho-D-gluconate + NAD(P)+
enzyme belongs to the oxidative pentose phosphate pathway
286822
Zea mays
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
-
Zea mays
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8
-
Ricinus communis
Other publictions for EC 1.1.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723699
Adem
Purification of rat kidney glu ...
Rattus norvegicus
Protein Expr. Purif.
81
1-4
2012
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
2
1
-
1
1
-
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-
-
-
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-
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2
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-
1
-
2
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723751
Fu
Cloning, expression and charac ...
Haemophilus parasuis, Haemophilus parasuis SH0165
Res. Vet. Sci.
93
57-62
2012
-
-
1
-
-
-
-
-
2
-
1
-
-
6
-
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1
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-
-
-
-
-
1
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-
1
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-
-
-
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-
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2
-
1
-
-
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710864
Laliotis
Enzymatic and mRNA transcript ...
Ovis aries
Anim. Biotechnol.
21
149-155
2010
-
-
-
-
-
-
-
-
-
-
-
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-
2
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712601
Akkemik
Effects of some drugs on human ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
25
476-479
2010
-
-
-
-
-
-
8
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
8
-
8
-
-
-
1
-
-
-
8
8
8
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710781
Hisar
-
Kinetic behaviour of glucose 6 ...
Oncorhynchus mykiss
Acta Vet. Brno
78
179-185
2009
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
3
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
688042
Ciftci
Effects of nicotine and vitami ...
Rattus norvegicus
J. Enzyme Inhib. Med. Chem.
23
261-265
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688050
Bulbul
Investigation of the effects o ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
23
418-423
2008
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
3
4
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667894
Choi
Glycoprotein isolated from Aca ...
Eleutherococcus senticosus
Biol. Pharm. Bull.
29
306-314
2006
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656434
Kindzelskii
6-phosphogluconate dehydrogena ...
Homo sapiens
J. Immunol.
172
6373-6381
2004
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654928
Tsanev
-
Malate dehydrogenase, alcohol ...
Tripsacum dactyloides, Zea mays, Zea mays x Tripsacum dactyloides
Biol. Plant.
45
517-522
2002
-
3
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286822
Bailey-Serres
Purification and characterizat ...
Candida boidinii, Nicotiana tabacum, Ricinus communis, Spinacia oleracea, Zea mays
Plant Physiol.
100
1580-1583
1992
-
-
-
-
-
-
1
7
6
4
1
5
-
6
-
-
2
-
-
4
1
-
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
6
4
1
5
-
-
-
2
-
4
1
-
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286823
Swezey
The use of caged substrates to ...
Lytechinus pictus, Strongylocentrotus purpuratus, Xenopus sp.
Exp. Cell Res.
201
366-372
1992
-
-
-
-
-
-
-
2
-
-
2
3
-
3
-
-
-
-
-
3
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
3
-
-
-
-
-
3
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286821
Bublitz
The topology of phosphoglucona ...
Rattus norvegicus
Arch. Biochem. Biophys.
259
22-28
1987
-
-
-
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286820
Hipkin
-
Inorganic nitrogen assimilatio ...
Ankistrodesmus braunii, Ankistrodesmus braunii C.C.A.P 202/7C, Chlamydomonas reinhardtii, 'Chlorella' fusca
Plant Sci.
41
155-160
1985
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286819
Ben-Bassat
Purification and properties of ...
Burkholderia cepacia, Candida boidinii, Enterococcus faecalis, Pseudomonas sp., Pseudomonas sp. C, Pseudomonas sp. W5
Biochim. Biophys. Acta
611
1-10
1980
-
-
-
-
-
-
5
5
-
2
3
6
-
8
-
-
2
-
-
-
2
-
9
1
-
-
-
-
2
-
-
4
-
-
-
-
-
-
4
-
-
-
-
5
-
5
-
2
3
6
-
-
-
2
-
-
2
-
9
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286818
Tanaka
-
Glucose-6-phosphate dehydrogen ...
Candida tropicalis
Agric. Biol. Chem.
40
1829-1835
1976
5
-
-
-
-
1
1
2
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
5
-
-
1
-
-
1
-
1
-
2
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286817
Lee
Purification and characterizat ...
Burkholderia cepacia, Enterococcus faecalis, Neurospora crassa, Pseudomonas aeruginosa
J. Bacteriol.
120
1043-1057
1974
-
-
-
-
-
-
5
7
-
-
8
4
-
5
-
-
1
-
-
-
2
1
8
2
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
7
-
-
8
4
-
-
-
1
-
-
2
1
8
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286816
Silverberg
-
Crystalline 6-phosphogluconate ...
Cyberlindnera jadinii, Ovis aries, Rattus norvegicus
Biochem. Soc. Trans.
1
1132-1134
1973
-
-
-
1
-
-
-
-
-
-
8
3
-
3
-
-
1
-
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
8
3
-
-
-
1
-
2
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286815
Lessie
Multiple forms of Pseudomonas ...
Burkholderia cepacia, Enterococcus faecalis
J. Bacteriol.
110
1107-1117
1972
-
-
-
-
-
-
1
5
-
-
-
2
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
5
-
-
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2
-
-
-
-
-
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2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
286814
Frampton
Carbohydrate oxidation by Pseu ...
Enterobacter cloacae, Leuconostoc mesenteroides, Pseudomonas fluorescens, Pseudomonas fluorescens A 3.12, Saccharomyces cerevisiae
J. Biol. Chem.
236
2571-2577
1961
-
-
-
-
-
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1
2
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5
-
5
-
-
2
-
-
-
1
1
13
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
5
-
-
-
2
-
-
1
1
13
-
-
-
-
-
2
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-