Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain DH5alpha as His-tagged enzyme | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 6.5, 37°C | Enterococcus faecalis | |
0.03 | - |
NADPH | pH 6.5, 37°C, (S)-3-hydroxy-3-methylglutaryl-CoA reduction | Enterococcus faecalis | |
0.22 | - |
CoA | pH 6.5, 37°C, mevaldehyde oxidation | Enterococcus faecalis | |
0.23 | - |
CoA | pH 6.5, 37°C, mevalonate oxidation | Enterococcus faecalis | |
0.25 | - |
NADPH | pH 6.5, 37°C, mevaldehyde reduction | Enterococcus faecalis | |
0.25 | - |
NADP+ | pH 6.5, 37°C, mevalonate oxidation | Enterococcus faecalis | |
0.66 | - |
mevaldehyde | pH 6.5, 37°C, oxidation | Enterococcus faecalis | |
0.67 | - |
NADP+ | pH 6.5, 37°C, mevaldehyde oxidation | Enterococcus faecalis | |
1 | - |
(R)-mevalonate | pH 6.5, 37°C, oxidation | Enterococcus faecalis | |
3.8 | - |
mevaldehyde | pH 6.5, 37°C, reduction | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Enterococcus faecalis | involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
acetyl-CoA | Enterococcus faecalis | - |
acetoacetyl-CoA + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
dual-function enzyme with acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase activities | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography | Enterococcus faecalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | via mevaldehyde as an intermediate product | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview | Enterococcus faecalis | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | overall reaction | Enterococcus faecalis | (R)-mevalonate + CoA + 2 NADP+ | - |
r | |
(S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | first step reaction | Enterococcus faecalis | mevaldehyde + CoA + NADP+ | - |
r | |
acetyl-CoA | - |
Enterococcus faecalis | acetoacetyl-CoA + CoA | - |
r | |
mevaldehyde + NADPH + H+ | second step reaction | Enterococcus faecalis | (R)-mevalonate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
about, acetoacetyl-coenzyme A synthesis and thiolysis | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA reduction | Enterococcus faecalis |
7 | - |
(R)-mevalonate oxidation | Enterococcus faecalis |
9.5 | - |
acetoacetyl-coenzyme A thiolysis | Enterococcus faecalis |
10.5 | - |
acetoacetyl-coenzyme A synthesis | Enterococcus faecalis |