BRENDA - Enzyme Database show
show all sequences of 1.1.1.254

Synthesis of poly-beta-hydroxybutyrate by Agrobacterium radiobacter after growth on D-carnitine

Kluettermann, K.; Tauchert, H.; Kleber, H.P.; Acta Biotechnol. 22, 261-269 (2002)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
Agrobacterium radiobacter can be used for conversion of the waste product L(-)-carnitine to poly-beta-hydroxybutyrate valuable as a biodegradable and biocompatible plastic
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-carnitine + NAD+
Agrobacterium tumefaciens
substrate is derived as a waste product from chemical production procedures of L(-)-carnitine
3-dehydrocarnitine + NADH + H+
formation of poly-beta-hydroxybutyrate as a storage product
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Reaction
Reaction
Commentary
Organism
(S)-carnitine + NAD+ = 3-dehydrocarnitine + NADH + H+
specific for the (S)-enantiomer of carnitine
Agrobacterium tumefaciens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
organism is able to grow on synthetic D(+)-carnitine, but not on physiological L(-)-carnitine, as sole carbon and nitrogen source
Agrobacterium tumefaciens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-carnitine + NAD+
-
654037
Agrobacterium tumefaciens
3-dehydrocarnitine + NADH + H+
-
-
-
?
(S)-carnitine + NAD+
substrate is derived as a waste product from chemical production procedures of L(-)-carnitine
654037
Agrobacterium tumefaciens
3-dehydrocarnitine + NADH + H+
formation of poly-beta-hydroxybutyrate as a storage product
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
specific for
Agrobacterium tumefaciens
Application (protein specific)
Application
Commentary
Organism
synthesis
Agrobacterium radiobacter can be used for conversion of the waste product L(-)-carnitine to poly-beta-hydroxybutyrate valuable as a biodegradable and biocompatible plastic
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
specific for
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-carnitine + NAD+
Agrobacterium tumefaciens
substrate is derived as a waste product from chemical production procedures of L(-)-carnitine
3-dehydrocarnitine + NADH + H+
formation of poly-beta-hydroxybutyrate as a storage product
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
organism is able to grow on synthetic D(+)-carnitine, but not on physiological L(-)-carnitine, as sole carbon and nitrogen source
Agrobacterium tumefaciens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-carnitine + NAD+
-
654037
Agrobacterium tumefaciens
3-dehydrocarnitine + NADH + H+
-
-
-
?
(S)-carnitine + NAD+
substrate is derived as a waste product from chemical production procedures of L(-)-carnitine
654037
Agrobacterium tumefaciens
3-dehydrocarnitine + NADH + H+
formation of poly-beta-hydroxybutyrate as a storage product
-
-
?
Other publictions for EC 1.1.1.254
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654037
Kluettermann
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Synthesis of poly-beta-hydroxy ...
Agrobacterium tumefaciens
Acta Biotechnol.
22
261-269
2002
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285734
Obon
Enzymatic cycling assay for D- ...
Agrobacterium sp.
Anal. Biochem.
274
34-39
1999
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9587
Setyahadi
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Production of L-carnitine from ...
Agrobacterium sp.
J. Mol. Catal. , B Enzym.
4
205-209
1998
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9588
Hanschmann
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Conversion of D-carnitine into ...
Agrobacterium sp.
Biotechnol. Lett.
19
679-682
1997
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9589
Hanschmann
Purification and characterizat ...
Agrobacterium sp.
Biochim. Biophys. Acta
1337
133-142
1997
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5
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285735
Setyahida
Purification and properties of ...
Agrobacterium sp. 525a
Biosci. Biotechnol. Biochem.
61
1055-1058
1997
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