BRENDA - Enzyme Database show
show all sequences of 1.1.1.234

Biochemical formation of anthocyanins in silk tissue of Zea mays

Halbwirth, H.; Martnes, S.; Wienand, U.; Forkmann, G.; Stich, K.; Plant Sci. 164, 489-495 (2003)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in yeast and petunia
Zea mays
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
slight inhibition at 1 mM, 50-70% inhibition at 5 mM
Zea mays
p-hydroxymercuribenzoate
complete inhibition at 1 mM
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-eriodictyol + NADPH
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
-
-
?
(2S)-flavanone + NADPH
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
apiforol + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
-
strains: Peterson Ac 1355 A-D (sm), al-line, and Pirat
-
Reaction
Reaction
Commentary
Organism
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH + H+
enzyme acts as flavone 4-reductase as well as dihydroflavonol 4-reductase
Zea mays
Source Tissue
Source Tissue
Commentary
Organism
Textmining
silk
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
657102
Zea mays
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
luteoforol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
-
657102
Zea mays
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
657102
Zea mays
apiforol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
apiforol + NADP+
-
-
-
?
additional information
enzyme also acts on 5-deoxydihydroflavonols
657102
Zea mays
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Zea mays
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Zea mays
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
can partially replace for NADPH
Zea mays
NADPH
-
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in yeast and petunia
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
can partially replace for NADPH
Zea mays
NADPH
-
Zea mays
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
slight inhibition at 1 mM, 50-70% inhibition at 5 mM
Zea mays
p-hydroxymercuribenzoate
complete inhibition at 1 mM
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-eriodictyol + NADPH
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
-
-
?
(2S)-flavanone + NADPH
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Zea mays
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
apiforol + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
silk
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
657102
Zea mays
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
luteoforol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
-
657102
Zea mays
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
657102
Zea mays
apiforol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
657102
Zea mays
apiforol + NADP+
-
-
-
?
additional information
enzyme also acts on 5-deoxydihydroflavonols
657102
Zea mays
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
-
Zea mays
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Zea mays
Other publictions for EC 1.1.1.234
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
-
-
1
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
654357
Fischer
Molecular cloning, substrate s ...
Malus domestica, Pyrus communis
Arch. Biochem. Biophys.
412
223-230
2003
-
-
1
-
-
-
-
-
-
-
-
6
-
7
-
-
-
2
-
2
-
-
10
-
4
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
10
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
657102
Halbwirth
-
Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
-
-
1
-
-
-
2
-
-
-
-
3
-
1
-
-
-
1
-
1
-
-
7
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
1
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286346
Stich
-
Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
-
-
-
-
-
1
5
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
438999
Stich
-
Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-