BRENDA - Enzyme Database show
show all sequences of 1.1.1.234

Molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from Malus domestica and Pyrus communis cultivars and the consequences for flavonoid metabolism

Fischer, T.C.; Halbwirth, H.; Meisel, B.; Stich, K.; Forkmann, G.; Arch. Biochem. Biophys. 412, 223-230 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in yeast strain InvSc1
Malus domestica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-eriodictyol + NADPH
Pyrus communis
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
?
(2S)-eriodictyol + NADPH
Malus domestica
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
?
(2S)-flavanone + NADPH
Pyrus communis
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-flavanone + NADPH
Malus domestica
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Pyrus communis
-
apiforol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Malus domestica
-
apiforol + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malus domestica
Q9XES5
strains M9 and Weirouge
-
Pyrus communis
-
strains Pyrodwarf, Conference and Abbe Fetel
-
Reaction
Reaction
Commentary
Organism
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH + H+
enzyme acts as flavone 4-reductase as well as dihydroflavonol 4-reductase
Malus domestica
(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH + H+
enzyme acts as flavone 4-reductase as well as dihydroflavonol 4-reductase
Pyrus communis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Malus domestica
-
leaf
low activity
Pyrus communis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
654357
Pyrus communis
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
-
654357
Malus domestica
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Pyrus communis
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Malus domestica
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
-
?
(2S)-flavanone + NADPH
-
654357
Pyrus communis
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
-
654357
Malus domestica
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Pyrus communis
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Malus domestica
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
654357
Pyrus communis
apiforol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
654357
Malus domestica
apiforol + NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
native enzyme
Malus domestica
25
-
native enzyme
Pyrus communis
40
-
recombinant enzyme from yeast
Malus domestica
40
-
recombinant enzyme from yeast
Pyrus communis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Malus domestica
6
-
-
Pyrus communis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Malus domestica
NADPH
-
Pyrus communis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in yeast strain InvSc1
Malus domestica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Malus domestica
NADPH
-
Pyrus communis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S)-eriodictyol + NADPH
Pyrus communis
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
?
(2S)-eriodictyol + NADPH
Malus domestica
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
?
(2S)-flavanone + NADPH
Pyrus communis
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-flavanone + NADPH
Malus domestica
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
(2S)-flavan-4-ol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Pyrus communis
-
apiforol + NADP+
-
-
?
(2S)-naringenin + NADPH + H+
Malus domestica
-
apiforol + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Malus domestica
-
leaf
low activity
Pyrus communis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-eriodictyol + NADPH
-
654357
Pyrus communis
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
-
654357
Malus domestica
luteoforol + NADP+
-
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Pyrus communis
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
-
?
(2S)-eriodictyol + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Malus domestica
luteoforol + NADP+
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
-
-
?
(2S)-flavanone + NADPH
-
654357
Pyrus communis
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
-
654357
Malus domestica
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Pyrus communis
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-flavanone + NADPH
reaction provides precursors for the synthesis of 3-deoxyanthocyanidins
654357
Malus domestica
(2S)-flavan-4-ol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
654357
Pyrus communis
apiforol + NADP+
-
-
-
?
(2S)-naringenin + NADPH + H+
-
654357
Malus domestica
apiforol + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
native enzyme
Malus domestica
25
-
native enzyme
Pyrus communis
40
-
recombinant enzyme from yeast
Malus domestica
40
-
recombinant enzyme from yeast
Pyrus communis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Malus domestica
6
-
-
Pyrus communis
Other publictions for EC 1.1.1.234
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725929
Li
Molecular characterization and ...
Saussurea medusa
Mol. Biol. Rep.
39
2991-2999
2012
-
-
1
-
-
-
-
-
-
-
-
1
-
6
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
654357
Fischer
Molecular cloning, substrate s ...
Malus domestica, Pyrus communis
Arch. Biochem. Biophys.
412
223-230
2003
-
-
1
-
-
-
-
-
-
-
-
6
-
7
-
-
-
2
-
2
-
-
10
-
4
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
10
-
4
-
-
-
2
-
-
-
-
-
-
-
-
-
657102
Halbwirth
-
Biochemical formation of antho ...
Zea mays
Plant Sci.
164
489-495
2003
-
-
1
-
-
-
2
-
-
-
-
3
-
1
-
-
-
1
-
1
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-
7
-
1
-
-
-
1
-
-
2
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-
-
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-
1
2
-
-
-
-
2
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-
-
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3
-
-
-
-
-
1
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
286346
Stich
-
Biosynthesis of 3-deoxyanthocy ...
Sinningia cardinalis
Biochemistry
27
785-789
1988
-
-
-
-
-
1
5
1
-
-
-
1
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
3
-
-
1
-
5
-
1
-
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
438999
Stich
-
Studies on columnidin biosynth ...
Columnea hybrida, Columnea hybrida Heklua
Z. Naturforsch. C
43
311-314
1988
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
6
-
-
-
-
-
1
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-
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