BRENDA - Enzyme Database show
show all sequences of 1.1.1.213

Rat NAD+-dependent 3alpha-hydroxysteroid dehydrogenase (AKR1C17): a member of the aldo-keto reductase family highly expressed in kidney cytosol

Sanai, M.; Endo, S.; Matsunaga, T.; Ishikura, S.; Tajima, K.; El-Kabbani, O.; Hara, A.; Arch. Biochem. Biophys. 464, 122-129 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3)
Rattus norvegicus
Engineering
Amino acid exchange
Commentary
Organism
E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Q270K
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Q270K/E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-androstene-3,17-dione
versus substrate, the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate
Rattus norvegicus
Ketamine
specific inhibitor for AKR1C17, but no inhibition of AKR1C9
Rattus norvegicus
NADH
the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.0021
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0024
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0027
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.004
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.022
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.023
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.038
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.42
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
expression of AKR1C17 and AKR1C9
Rattus norvegicus
5829
-
additional information
subcellular localization of isozymes, overview
Rattus norvegicus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
recombinant AKR1C17, gel filtration
Rattus norvegicus
37000
-
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
female Wistar rats
-
Purification (Commentary)
Commentary
Organism
recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3)
Rattus norvegicus
Reaction
Reaction
Commentary
Organism
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+
the enzyme shows a sequential ordered reaction mechanism, where NAD+ binds to the enzyme first and NADH leaves last
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla
Rattus norvegicus
-
additional information
tissue distribution of isozymes, overview
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
0.23
-
purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androsten-3alpha-ol-17-one + NADH + H+
AKR1C17
684676
Rattus norvegicus
4-androsten-3alpha,17beta-diol + NAD+
-
-
-
r
4-androstene-3,17-dione + NADH + H+
low activity
684676
Rattus norvegicus
4-androsten-3alpha-ol-17-one + NAD+
-
-
-
r
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
684676
Rattus norvegicus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
r
5alpha-androstane-3,17-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-androstan-3alpha-ol-17-one + NAD+
AKR1C9
-
-
r
5alpha-dihydrotestosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
5alpha-pregnane-20alpha-ol-3-one + NADH
-
684676
Rattus norvegicus
5alpha-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5alpha-pregnane-3,20-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
5beta-androstan-3alpha-ol-17-one + NADH
-
684676
Rattus norvegicus
5beta-androstane-3alpha,17beta-diol + NAD+
-
-
-
r
5beta-androstane-3,17-dione + NADH
-
684676
Rattus norvegicus
5beta-androstan-3alpha-ol-17-one + NAD+
-
-
-
r
5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5beta-pregnane-3,20-dione + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH
the enzyme produces both R- and S-form products in a ratio of 2.3 : 1
684676
Rattus norvegicus
?
-
-
-
?
9,10-phenanthrenequinone + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
beta-muricholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
chenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
cholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
dehydrolithocholic acid + NADH + H+
-
684676
Rattus norvegicus
?
-
-
-
?
deoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
diacetyl + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycochenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycoursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
hyodeoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
isatin + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
lithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
additional information
substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone
684676
Rattus norvegicus
?
-
-
-
-
murocholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
taurolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
tauroursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
testosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
ursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.022
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.047
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.1
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.105
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.115
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.167
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.183
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.283
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
9.5
recombinant enzyme
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
11
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+
preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADPH
-
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0022
-
4-androstene-3,17-dione
-
Rattus norvegicus
0.017
-
NADH
-
Rattus norvegicus
0.018
-
4-androstene-3,17-dione
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant AKR1C17s in Escherichia coli strain BL21(DE3)
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred by AKR1C17, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+
preferred by AKR1C9, dependent on, residues Gln270 and Glu276 are important for cofactor specificity, overview
Rattus norvegicus
NADPH
-
Rattus norvegicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Q270K
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Q270K/E276R
site-directed mutagenesis, the mutation alters the cofactor specificity of AKR1C17 from NAD+ to NADP+, the switch is analogy th the residues of AKRc9 and its cofactor specificity, overview
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-androstene-3,17-dione
versus substrate, the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate
Rattus norvegicus
Ketamine
specific inhibitor for AKR1C17, but no inhibition of AKR1C9
Rattus norvegicus
NADH
the products, 4-androstene-3,17-dione and NADH, inhibit the activity uncompetitively and competitively, respectively, with respect to NAD+ in the presence of a saturated concentration of 0.004 mM of the substrate
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0022
-
4-androstene-3,17-dione
-
Rattus norvegicus
0.017
-
NADH
-
Rattus norvegicus
0.018
-
4-androstene-3,17-dione
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.0021
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0024
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.0027
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.004
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.022
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.023
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.038
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.42
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
expression of AKR1C17 and AKR1C9
Rattus norvegicus
5829
-
additional information
subcellular localization of isozymes, overview
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
recombinant AKR1C17, gel filtration
Rattus norvegicus
37000
-
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant wild-type and mutant AKR1C17s from Escherichia coli strain BL21(DE3)
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
specific expression of AKR1C17, two enzymes are expressed more highly in renal cortex than in the medulla
Rattus norvegicus
-
additional information
tissue distribution of isozymes, overview
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.07
-
recombinant AKR1C17 in cell extract, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
0.23
-
purified recombinant AKR1C17, substrate 4-androsten-3alpha-ol-17-one
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-androsten-3alpha-ol-17-one + NADH + H+
AKR1C17
684676
Rattus norvegicus
4-androsten-3alpha,17beta-diol + NAD+
-
-
-
r
4-androstene-3,17-dione + NADH + H+
low activity
684676
Rattus norvegicus
4-androsten-3alpha-ol-17-one + NAD+
-
-
-
r
5alpha-androstan-3alpha-ol-17-one + NADH + H+
-
684676
Rattus norvegicus
5beta-androstan-3alpha,17beta-diol + NAD+
-
-
-
r
5alpha-androstane-3,17-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-androstan-3alpha-ol-17-one + NAD+
AKR1C9
-
-
r
5alpha-dihydrotestosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
5alpha-pregnane-20alpha-ol-3-one + NADH
-
684676
Rattus norvegicus
5alpha-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5alpha-pregnane-3,20-dione + NADH
low activity
684676
Rattus norvegicus
5alpha-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
5beta-androstan-3alpha-ol-17-one + NADH
-
684676
Rattus norvegicus
5beta-androstane-3alpha,17beta-diol + NAD+
-
-
-
r
5beta-androstane-3,17-dione + NADH
-
684676
Rattus norvegicus
5beta-androstan-3alpha-ol-17-one + NAD+
-
-
-
r
5beta-pregnane-20alpha-ol-3-one + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnane-3,20alpha-diol + NAD+
-
-
-
?
5beta-pregnane-3,20-dione + NADH + H+
-
684676
Rattus norvegicus
5beta-pregnan-3alpha-ol-20-one + NAD+
-
-
-
r
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one + NADH
the enzyme produces both R- and S-form products in a ratio of 2.3 : 1
684676
Rattus norvegicus
?
-
-
-
?
9,10-phenanthrenequinone + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
beta-muricholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
chenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
cholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
dehydrolithocholic acid + NADH + H+
-
684676
Rattus norvegicus
?
-
-
-
?
deoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
diacetyl + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycochenodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
glycoursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
hyodeoxycholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
isatin + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
lithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
additional information
substrate specificty, overview, recombinant AKR1C17 efficiently oxidizes 3alpha-hydroxysteroids and diverse bile acids using NAD+ as the preferred coenzyme, no activity with progesterone, cortisone, and aldosterone. The enzyme also reduces non-steroidal alpha-dicarbonyl compounds such as 9,10-phenanethrenequinone, isatin, 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one and diacetyl, but does not show significant activities towards 4-nitrobenzaldehyde and 4-nitroacetophenone
684676
Rattus norvegicus
?
-
-
-
-
murocholic acid + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
taurolithocholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
tauroursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
testosterone + NADH
low activity
684676
Rattus norvegicus
?
-
-
-
?
ursodeoxycholic acid + NADH
-
684676
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, recombinant AKR1C17, SDS-PAGE
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cofactor kinetics of wild-type and mutant enzymes, overview
Rattus norvegicus
0.022
-
Dehydrolithocholic acid
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.047
-
5beta-androstane-3,17-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.1
-
6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.105
-
5beta-pregnane-3,20-dione
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.115
-
(5beta,20R)-20-hydroxypregnan-3-one
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.167
-
diacetyl
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.183
-
9,10-phenanthrenequinone
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
0.283
-
isatin
pH 7.4, 25°C, recombinant wild-type AKR1C17
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
9.5
recombinant enzyme
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
11
-
Rattus norvegicus
Other publictions for EC 1.1.1.213
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724719
Matsunaga
Pathophysiological roles of al ...
Homo sapiens
Chem. Biol. Interact.
202
234-242
2013
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1
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-
1
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1
1
1
1
1
1
724915
Heinrich
Synthesis and structure-activi ...
Homo sapiens
Eur. J. Med. Chem.
62
738-744
2013
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1
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3
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724916
Gazvoda
2,3-diarylpropenoic acids as s ...
Homo sapiens
Eur. J. Med. Chem.
62
89-97
2013
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-
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1
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4
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1
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723866
Jackson
Structure of AKR1C3 with 3-phe ...
Homo sapiens
Acta Crystallogr. Sect. F
68
409-413
2012
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1
1
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1
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1
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724533
Chen
Crystal structures of AKR1C3 c ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
3492-3497
2012
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724538
Sinreih
N-Benzoyl anthranilic acid der ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
5948-5951
2012
-
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4
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1
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725153
Miller
Aldo-keto reductase family 1 m ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
5
278-289
2012
-
1
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8
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1
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8
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-
725671
Brozic
Selective inhibitors of aldo-k ...
Homo sapiens
J. Med. Chem.
55
7417-7424
2012
-
-
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1
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2
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1
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2
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1
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2
2
2
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1
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-
-
-
725825
Byrns
Overexpression of aldo-keto re ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
130
7-15
2012
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1
1
-
-
-
724517
Adeniji
Discovery of substituted 3-(ph ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
1464-1468
2011
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3
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1
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1
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3
3
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1
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-
725152
Zakharov
Suppressed expression of type ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
3
608-617
2010
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1
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1
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2
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1
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1
-
-
697265
Skarydova
AKR1C3 as a potential target f ...
Homo sapiens
Chem. Biol. Interact.
178
138-144
2009
-
1
1
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-
-
15
-
-
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2
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1
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1
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7
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1
1
1
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-
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7
15
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1
-
-
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-
-
-
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-
-
-
-
-
-
-
-
685529
Uwai
Electronic effects of para-sub ...
Rattus norvegicus
Bioorg. Med. Chem.
16
1084-1089
2008
-
-
-
-
-
-
-
1
-
-
-
-
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2
-
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-
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2
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8
-
1
-
-
-
1
-
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1
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
2
-
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
688137
Azzarello
Tissue distribution of human A ...
Homo sapiens, Rattus norvegicus
J. Histochem. Cytochem.
56
853-861
2008
-
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1
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-
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6
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2
2
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7
-
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14
-
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2
1
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-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
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6
-
2
2
-
-
-
-
-
14
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689169
Meyer
The biological activity of 3al ...
Rattus norvegicus
Neurobiol. Dis.
30
30-41
2008
-
1
-
-
-
-
-
-
-
-
1
1
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3
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2
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2
1
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2
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1
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2
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1
1
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2
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2
1
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-
697310
Shultz
Fjord-region benzo[g]chrysene- ...
Rattus norvegicus
Chem. Res. Toxicol.
21
668-677
2008
-
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1
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8
-
2
2
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-
-
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3
-
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1
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2
1
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2
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1
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1
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1
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1
1
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8
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2
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2
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1
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2
1
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2
-
1
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-
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1
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-
-
-
-
-
-
-
-
684676
Sanai
Rat NAD+-dependent 3alpha-hydr ...
Rattus norvegicus
Arch. Biochem. Biophys.
464
122-129
2007
-
-
1
-
3
-
3
9
3
-
2
-
-
2
-
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1
1
-
3
2
-
31
1
1
-
-
9
1
1
-
4
3
-
-
-
-
1
4
-
3
-
-
3
3
9
3
-
2
-
-
-
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1
-
3
2
-
31
1
1
-
-
9
1
1
-
-
-
-
-
-
-
-
687635
Cooper
Elucidation of a complete kine ...
Rattus norvegicus
J. Biol. Chem.
282
33484-33493
2007
-
-
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3
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1
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2
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1
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2
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1
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1
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2
1
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2
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2
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3
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1
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2
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1
-
1
-
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2
1
-
-
-
-
-
-
-
-
-
687639
McCarthy
3-ketosteroid reductase activi ...
Rattus norvegicus
J. Biol. Chem.
282
34003-34012
2007
1
-
1
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-
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-
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-
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2
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2
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2
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3
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2
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1
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1
2
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2
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2
-
-
3
-
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-
-
-
-
-
-
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-
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687642
Chang
Mechanism of proton transfer i ...
Comamonas testosteroni
J. Biol. Chem.
282
34306-34314
2007
-
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1
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2
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3
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1
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1
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1
1
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1
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2
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1
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1
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2
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1
2
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2
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3
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1
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1
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1
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2
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1
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1
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687870
Hoffmann
Understanding oligomerization ...
Comamonas testosteroni
J. Biotechnol.
129
131-139
2007
-
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1
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1
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-
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1
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1
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2
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1
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1
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1
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2
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-
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-
668392
Matsunaga
Multiplicity of mammalian redu ...
Homo sapiens
Drug Metab. Pharmacokinet.
21
1-18
2006
-
-
1
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-
-
-
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1
-
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16
-
4
-
-
-
-
-
4
-
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30
-
-
-
-
-
-
-
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1
-
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-
-
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1
1
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-
-
-
-
-
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1
-
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16
-
-
-
-
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4
-
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30
-
-
-
-
-
-
-
-
-
-
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-
-
-
672088
Jin
Multiple steps determine the o ...
Homo sapiens
Biochemistry
45
13054-13063
2006
-
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1
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3
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1
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1
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1
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1
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3
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1
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2
1
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2
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1
2
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3
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1
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1
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1
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3
-
1
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2
1
-
-
-
-
-
-
-
-
-
673349
Papari-Zareei
Arginine 276 controls the dire ...
Rattus norvegicus
Endocrinology
147
1591-1597
2006
-
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1
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3
-
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1
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1
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3
-
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1
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4
1
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2
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1
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1
1
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3
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1
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1
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4
1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673967
Rani
Discrete analysis of bile acid ...
Comamonas testosteroni
Indian J. Biochem. Biophys.
43
98-104
2006
-
1
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1
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-
-
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-
-
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3
-
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
674674
Nakamura
Apo- and holo-structures of 3a ...
Pseudomonas sp., Pseudomonas sp. B-0831
J. Biol. Chem.
281
31876-31884
2006
-
-
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1
-
-
-
-
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1
2
-
8
-
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-
-
-
-
-
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2
2
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-
-
-
-
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1
-
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-
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1
1
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1
2
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2
2
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