Protein Variants | Comment | Organism |
---|---|---|
N167A | site-directed mutagenesis, most impaired enzyme | Rattus norvegicus |
Q190A | site-directed mutagenesis, decreased binding affinity to NADP(H), only binding of cofactor is affected, residue is located at the catalytic cente | Rattus norvegicus |
R276M | elimination of salt bridge between Arg276 and the 2'-phosphate of AMP results in 100fold decrease of the affinity to NADP(H) | Rattus norvegicus |
S166A | site-directed mutagenesis, decreased binding affinity to NADP(H), only binding of cofactor is affected, residue is located at the catalytic center | Rattus norvegicus |
Y216S | site-directed mutagenesis, decreased binding affinity to NADP(H), only binding of cofactor is affected, residue is located at the catalytic cente | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | 0.0039 | 5alpha-androstan-3,17-dione | NADPH | Rattus norvegicus | |
0.0013 | 0.0441 | 3alpha-hydroxy-5alpha-androstan-17-one | NAD+ | Rattus norvegicus | |
0.0022 | 0.0081 | NADPH | - |
Rattus norvegicus | |
0.0039 | 0.0304 | 5alpha-androstan-3,17-dione | NADH | Rattus norvegicus | |
0.004 | 0.821 | NAD+ | - |
Rattus norvegicus | |
0.0041 | 0.024 | 3alpha-hydroxy-5alpha-androstan-17-one | NADP+ | Rattus norvegicus | |
0.0052 | 0.1515 | NADP+ | - |
Rattus norvegicus | |
0.0057 | 0.0312 | NADH | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
1 * 37000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | Rattus norvegicus | - |
3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
additional information | Rattus norvegicus | biosynthesis and inactivation of steroid hormones, physiological role is to inactivate circulating androgens, progestins and glucocorticoids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P23457 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange and Blue Sepharose column | Rattus norvegicus |
N167A chromatofocusing and gel filtration introduced between the to others chromatography steps | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | aldoketoreductase superfamily | Rattus norvegicus | |
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | bi bi mechanism, pyridine nucleotide binds first and leaves last | Rattus norvegicus | |
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | ordered and sequential mechanism, nucleotide binds first and leaves last | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
Q190A mutant, androsterone oxidation with NAD+ | Rattus norvegicus |
0.1 | - |
S166A mutant, androsterone oxidation with NAD+ | Rattus norvegicus |
0.81 | - |
Y216S mutant, androsterone oxidation with NADP+ | Rattus norvegicus |
1.55 | - |
recombinant wild type enzyme, androsterone oxidation with NAD+ | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxy-5alpha-androstan-3-one + NAD(P)H + H+ | - |
Rattus norvegicus | 3alpha,17beta-dihydroxy-5alpha-androstan + NAD(P)+ | - |
? | |
5alpha-androstan-3,17-dione + NAD(P)H | - |
Rattus norvegicus | 3alpha-hydroxy-5alpha-androstan-17-one + NAD(P)+ | - |
r | |
additional information | biosynthesis and inactivation of steroid hormones, physiological role is to inactivate circulating androgens, progestins and glucocorticoids | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | transfer of the 4-pro-R hydrogen from A-face of cofactor to the beta-face of the steroid substrate. Different binding modes for NAD(H) and NADP(H) | Rattus norvegicus | |
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus | |
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |