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Literature summary for 1.1.1.14 extracted from

  • Darmanin, C.; Iwata, T.; Carper, D.A.; Sparrow, L.G.; Chung, R.P.; El-Kabbani, O.
    Expression, purification and preliminary crystallographic analysis of human sorbitol dehydrogenase (2003), Acta Crystallogr. Sect. D, 59, 558-560.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enyme, hanging drop vapour diffusion method, 22°C, enzyme is complexed with cofactor NAD+ in a molar ratio 1:6, 0.0025 ml protein solution mixed with 0.002 ml well solution containing 0.1 M Tris, pH 8.6, 0.2 M sodium acetate, 10% PEG 3350, and with 0.0005 ml 30% v/v 2-methyl-2,4-pentanediol, 1 week, X-ray diffraction structure determination and analysis at 2.75 A resolution Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli by ammonium sulfate precipitation, anion-exchange and dye affinity cromatography, 23fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.62
-
purified recombinant enzyme Homo sapiens

Synonyms

Synonyms Comment Organism
SDH
-
Homo sapiens
sorbitol dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.9
-
assay at Homo sapiens