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Literature summary for 1.1.1.14 extracted from

  • Rehg, J.E.; Torack, R.M.
    Partial purification and characterization of sorbitol dehydrogenase from rat brain (1977), J. Neurochem., 28, 655-660.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine fructose reduction at pH 7.4, inhibits at pH 9.0 Rattus norvegicus
ZnSO4 3.5 mM reverses EDTA inhibition Rattus norvegicus

General Stability

General Stability Organism
bovine serum albumin stabilizes at 4°C Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dimercaptopropanol
-
Rattus norvegicus
cysteine
-
Rattus norvegicus
EDTA at pH 7.4, not at pH 9.0, fructose reduction Rattus norvegicus
iodoacetate
-
Rattus norvegicus
p-hydroxymercuribenzoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
D-sorbitol
-
Rattus norvegicus
0.4
-
D-xylitol
-
Rattus norvegicus
1.3
-
D-ribitol
-
Rattus norvegicus
16
-
D-mannitol
-
Rattus norvegicus
16.7
-
D-galactitol
-
Rattus norvegicus
20
-
L-arabitol
-
Rattus norvegicus
54
-
D-ribulose
-
Rattus norvegicus
77
-
D-fructose
-
Rattus norvegicus
110
-
L-sorbose
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sorbitol + NAD+ Rattus norvegicus
-
D-fructose + NADH
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-85°C, good preservation Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+
-
Rattus norvegicus D-sorbitol + NAD+
-
?
D-galactitol + NAD+
-
Rattus norvegicus D-tagatose + NADH
-
?
D-mannitol + NAD+
-
Rattus norvegicus ?
-
?
D-ribitol + NAD+
-
Rattus norvegicus ?
-
?
D-ribulose + NADH
-
Rattus norvegicus ?
-
?
D-sorbitol + NAD+
-
Rattus norvegicus D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Rattus norvegicus ?
-
?
L-arabitol + NAD+
-
Rattus norvegicus ?
-
?
L-sorbose + NADH
-
Rattus norvegicus ?
-
?
sorbitol + NAD+
-
Rattus norvegicus D-fructose + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8 D-fructose reduction Rattus norvegicus
9 10 sorbitol oxidation Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 at pH 6 about 70% of maximal activity, at pH 9 about 40%, fructose reduction Rattus norvegicus
7.5 10 at pH 7.5 about 70% of maximal activity, at pH 10 about 100%, sorbitol oxidation Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus
NADH
-
Rattus norvegicus
NADP+ cannot replace NAD+ Rattus norvegicus
NADPH
-
Rattus norvegicus