BRENDA - Enzyme Database show
show all sequences of 1.1.1.133

Characterization of dTDP-4-dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar typhimurium LT2

Graninger, M.; Nidetzky, B.; Heinrichs, D.E.; Whitfield, C.; Messner, P.; J. Biol. Chem. 274, 25069-25077 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in Escherichia coli BL21(lambdaDE3)
Salmonella enterica
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
up to 0.3 mM, 70% loss of enzyme activity
Salmonella enterica
additional information
full activity restored with up to 0.3 mM MgCl2
Salmonella enterica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.106
-
NADH
+/-0.018, pH 7.0
Salmonella enterica
0.21
-
NADH
+/-0.004, pH 7.0
Salmonella enterica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32600
-
calculated from nucleotide sequence data
Salmonella enterica
34800
-
SDS-PAGE
Salmonella enterica
41500
-
gel filtration
Salmonella enterica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica
-
serovar typhimurium LT2
-
Purification (Commentary)
Commentary
Organism
affinity and ion exchange chromatography
Salmonella enterica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
44
-
-
Salmonella enterica
Storage Stability
Storage Stability
Organism
-20C, 44% glycerol
Salmonella enterica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
-
285846
Salmonella enterica
dTDP-beta-L-rhamnose + NAD+
-
-
-
-
dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H
-
285846
Salmonella enterica
dTDP-L-rhamnose + NAD(P)+
-
285846
Salmonella enterica
r
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
stable below
Salmonella enterica
37
-
pH 7.0, 75% activity loss
Salmonella enterica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25
-
NADH
+/-48
Salmonella enterica
29
-
NADPH
+/-66
Salmonella enterica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of dTDP-6-deoxy-L-lyxo-4-hexulose
Salmonella enterica
10
-
oxidation of dTDP-L-rhamnose
Salmonella enterica
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
37C, 20 min, 75% activity loss
Salmonella enterica
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica
NADH
preferred
Salmonella enterica
NADP+
-
Salmonella enterica
NADPH
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in Escherichia coli BL21(lambdaDE3)
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica
NADH
preferred
Salmonella enterica
NADP+
-
Salmonella enterica
NADPH
-
Salmonella enterica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
up to 0.3 mM, 70% loss of enzyme activity
Salmonella enterica
additional information
full activity restored with up to 0.3 mM MgCl2
Salmonella enterica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.106
-
NADH
+/-0.018, pH 7.0
Salmonella enterica
0.21
-
NADH
+/-0.004, pH 7.0
Salmonella enterica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32600
-
calculated from nucleotide sequence data
Salmonella enterica
34800
-
SDS-PAGE
Salmonella enterica
41500
-
gel filtration
Salmonella enterica
Purification (Commentary) (protein specific)
Commentary
Organism
affinity and ion exchange chromatography
Salmonella enterica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
44
-
-
Salmonella enterica
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 44% glycerol
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
-
285846
Salmonella enterica
dTDP-beta-L-rhamnose + NAD+
-
-
-
-
dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H
-
285846
Salmonella enterica
dTDP-L-rhamnose + NAD(P)+
-
285846
Salmonella enterica
r
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
stable below
Salmonella enterica
37
-
pH 7.0, 75% activity loss
Salmonella enterica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
25
-
NADH
+/-48
Salmonella enterica
29
-
NADPH
+/-66
Salmonella enterica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of dTDP-6-deoxy-L-lyxo-4-hexulose
Salmonella enterica
10
-
oxidation of dTDP-L-rhamnose
Salmonella enterica
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
-
37C, 20 min, 75% activity loss
Salmonella enterica
Other publictions for EC 1.1.1.133
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724529
Wang
Novel inhibitors of Mycobacter ...
Mycobacterium tuberculosis
Bioorg. Med. Chem. Lett.
21
7064-7067
2011
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-
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4
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3
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4
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4
4
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716065
Parakkottil Chothi
Identification of an L-rhamnos ...
Acanthamoeba polyphaga Mimivirus
J. Virol.
84
8829-8838
2010
-
-
1
-
-
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1
-
1
2
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1
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1
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1
1
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1
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1
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1
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1
1
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1
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1
2
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1
-
1
1
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-
1
-
1
-
-
-
-
-
-
-
-
-
661486
Elling
An enzyme module system for th ...
Salmonella enterica
ChemBioChem
6
1423-1430
2005
-
1
1
-
-
-
-
-
-
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-
1
-
1
-
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-
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2
-
1
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1
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1
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1
1
1
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1
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2
-
1
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1
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-
-
-
-
668041
Kang
Preparative synthesis of dTDP- ...
Mesorhizobium loti
Biotechnol. Bioeng.
93
21-27
2005
-
1
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
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-
1
-
-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657064
Watt
A bifunctional 3,5-epimerase/4 ...
Arabidopsis thaliana
Plant Physiol.
134
1337-1346
2004
-
-
1
-
-
-
1
2
-
-
2
-
-
1
-
-
1
-
-
4
-
1
4
1
1
1
-
-
-
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
2
-
-
2
-
-
-
-
1
-
4
-
1
4
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
657358
Blankenfeldt
Variation on a theme of SDR: d ...
Salmonella enterica
Structure
10
773-786
2002
-
-
-
1
-
-
-
-
-
1
-
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2
-
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1
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1
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285845
Nakano
Thymidine diphosphate-6-deoxy- ...
Aggregatibacter actinomycetemcomitans, Escherichia coli, Escherichia coli O45
J. Biol. Chem.
275
6806-6812
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
-
1
2
-
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2
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1
2
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2
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1
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1
2
-
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-
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-
-
-
-
-
-
285844
Giraud
-
Overexpression, purification, ...
Escherichia coli, Escherichia coli K12, Salmonella enterica, Shigella flexneri, Streptomyces mutans, Xanthomonas campestris
Acta Crystallogr. Sect. D
55
2043-2046
1999
-
-
1
1
-
-
-
-
-
-
5
6
-
166
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
5
-
-
-
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1
5
1
-
-
-
-
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5
6
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285846
Graninger
Characterization of dTDP-4-deh ...
Salmonella enterica
J. Biol. Chem.
274
25069-25077
1999
-
-
1
-
-
-
2
2
-
-
3
-
-
2
-
-
1
-
-
-
1
1
2
-
-
-
2
2
2
-
1
4
-
-
-
-
-
1
4
-
-
-
-
2
-
2
-
-
3
-
-
-
-
1
-
-
1
1
2
-
-
-
2
2
2
-
1
-
-
-
-
-
-
-
285843
Melo
The mechanism of 6-deoxyhexose ...
Pseudomonas aeruginosa
J. Biol. Chem.
243
1475-1478
1968
-
-
-
-
-
1
-
-
-
-
-
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1
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1
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-
1
-
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1
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1
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1
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1
-
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1
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