Information on EC 1.1.1.133 - dTDP-4-dehydrorhamnose reductase

New: Word Map on EC 1.1.1.133
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.1.133
-
RECOMMENDED NAME
GeneOntology No.
dTDP-4-dehydrorhamnose reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-beta-L-rhamnose + NADP+ = dTDP-4-dehydro-beta-L-rhamnose + NADPH + H+
show the reaction diagram
in the reverse direction, reduction on the 4-position of the hexose moiety takes place only while the substrate is bound to another enzyme which catalyzes epimerization at C-3 and C-5; the complex has been referred to as dTDP-L-rhamnose synthase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
dTDP-L-rhamnose biosynthesis I
-
-
dTDPLrhamnose biosynthesis
-
-
Metabolic pathways
-
-
Polyketide sugar unit biosynthesis
-
-
Streptomycin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
dTDP-6-deoxy-beta-L-mannose:NADP+ 4-oxidoreductase
In the reverse direction, reduction on the 4-position of the hexose moiety takes place only while the substrate is bound to another enzyme that catalyses epimerization at C-3 and C-5; the complex has been referred to as dTDP-L-rhamnose synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
37250-64-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional 3,5-epimerase/4-keto reductase
Swissprot
Manually annotated by BRENDA team
K12
-
-
Manually annotated by BRENDA team
O45
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ATCC 7700
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Streptomyces mutans
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+
dTDP-beta-L-rhamnose + NAD+
show the reaction diagram
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
dTDP-beta-L-rhamnose + NADP+
show the reaction diagram
-
-
-
?
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH + H+
dTDP-L-rhamnose + NADP+
show the reaction diagram
-
-
-
?
dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H
dTDP-L-rhamnose + NAD(P)+
show the reaction diagram
-
-
-
r
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
dTDP-L-rhamnose + NADP+
show the reaction diagram
dTDP-6-deoxy-L-mannose + NADP+
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
show the reaction diagram
UDP-4-dehydro-6-deoxy-D-glucose + NADPH
UDP-beta-L-rhamnose + NADP+
show the reaction diagram
-
-
-
?
UDP-4-keto-6-deoxy-D-glucose + NADPH + H+
UDP-L-rhamnose + NADP+
show the reaction diagram
-
product identification by electrospray ionization-mass spectrometry and gas chromatography mass spectrometry
-
?
additional information
?
-
no substrate: GDP-4-keto-6-deoxy-mannose, UDP-glucose, dTDP-glucose, UDP-xylose, GDP-fucose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH
dTDP-L-rhamnose + NADP+
show the reaction diagram
dTDP-6-deoxy-L-mannose + NADP+
dTDP-4-dehydro-6-deoxy-L-mannose + NADPH
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
situated at dimer interface
additional information
divalent metal ions do not alter activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6,7-trihydroxy-9-(2-hydroxyphenyl)-3H-xanthen-3-one
-
-
2,6,7-trihydroxy-9-methyl-3H-xanthen-3-one
-
-
2,6,7-trihydroxy-9-phenyl-3H-xanthen-3-one
-
-
5-ethenyl-1-methyl-9,10-dihydrophenanthrene-2,7-diol
-
-
EDTA
-
up to 0.3 mM, 70% loss of enzyme activity
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0169
dTDP-4-keto-6-deoxy-D-glucose
pH 8.5, 30C
0.106 - 0.21
NADH
0.009
NADPH
pH 8.5, 30C
1.83
UDP-4-keto-6-deoxy-D-glucose
25C, pH 7.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25
NADH
Salmonella enterica
-
+/-48
29
NADPH
Salmonella enterica
-
+/-66
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021
2,6,7-trihydroxy-9-(2-hydroxyphenyl)-3H-xanthen-3-one
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0015
2,6,7-trihydroxy-9-methyl-3H-xanthen-3-one
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0009
2,6,7-trihydroxy-9-phenyl-3H-xanthen-3-one
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
0.0025
5-ethenyl-1-methyl-9,10-dihydrophenanthrene-2,7-diol
Mycobacterium tuberculosis
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
reduction of dTDP-6-deoxy-L-lyxo-4-hexulose
7.8
-
assay at
10
-
oxidation of dTDP-L-rhamnose
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
about 35% of maximum activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
present in all tissues examined
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31900
-
Y4 rmlD gene product, SDS-PAGE
32400
-
NCTC 9710 rmlD gene product, SDS-PAGE
32540
-
mass spectrometry, native protein
32560
-
deduced from sequence
32600
-
calculated from nucleotide sequence data
32730
-
mass spectrometry, selenomethionine-enriched protein
34800
-
SDS-PAGE
41500
-
gel filtration
49500
-
light-scattering
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 35500, deduced from gene sequence, x * 35000, SDS-PAGE
monomer or dimer
x * 33000, UGER, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apo-enzyme, and in complex with NADH, NADPH or NADPH/dTDP-L-rhamnose
-
sitting drop vapour-diffusion method, 6 d
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
37C, 20 min, 75% activity loss
285846
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable below
37
-
pH 7.0, 75% activity loss
50
-
NADPH more than NADP+ prevents heat denaturation during 5 and 8 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
NADPH and to a lesser degree NADP+ prevent denaturation at 50C, NAD+ and NADH are less effective
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 44% glycerol
-
-20C, purified protein, 50% glycerol
-
-20C, repeated freezing and thawing, loss of more than 60% of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity and ion exchange chromatography
-
ammonium sulfate precipitation, affinity chromatography, co-purification of enzyme I and II
-
anion-exchange, hydrophobic chromatography
-
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
gene L780, transcription profiling of UGER, phylogenetic analysis
gene rmlD, expression in Escherichia coli strain BL21(DE3)
-
native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3)
-
overexpressed in Escherichia coli BL21(lambdaDE3)
-
overexpression in Escherichia coli DH5alpha, ER2566, BL21(DE3)
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
Show AA Sequence (3051 entries)
Please use the Sequence Search for a certain query.