BRENDA - Enzyme Database show
show all sequences of 1.1.1.114

Purification of a specific D-Apiitol dehydrogenase from a Micrococcus isolated from the surface of germinating parsley seeds

Hanna, R.; Picken, M.; Mendicino, J.; Biochim. Biophys. Acta 315, 259-271 (1973)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADH
-
Micrococcus sp.
0.35
-
NAD+
-
Micrococcus sp.
11.6
-
D-Apiitol
-
Micrococcus sp.
71.4
-
D-apiose
-
Micrococcus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
sucrose density centrifugation, gel filtration
Micrococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Micrococcus sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Micrococcus sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.102
-
-
Micrococcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-apiitol + NAD+
-
285765
Micrococcus sp.
D-apiose + NADH
-
-
-
-
meso-erythritol + NAD+
-
285765
Micrococcus sp.
D-erythrose + NADH
-
285765
Micrococcus sp.
-
myo-inositol + NAD+
-
285765
Micrococcus sp.
keto-inositol + NADH
-
285765
Micrococcus sp.
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Micrococcus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Micrococcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADH
-
Micrococcus sp.
0.35
-
NAD+
-
Micrococcus sp.
11.6
-
D-Apiitol
-
Micrococcus sp.
71.4
-
D-apiose
-
Micrococcus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
sucrose density centrifugation, gel filtration
Micrococcus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Micrococcus sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.102
-
-
Micrococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-apiitol + NAD+
-
285765
Micrococcus sp.
D-apiose + NADH
-
-
-
-
meso-erythritol + NAD+
-
285765
Micrococcus sp.
D-erythrose + NADH
-
285765
Micrococcus sp.
-
myo-inositol + NAD+
-
285765
Micrococcus sp.
keto-inositol + NADH
-
285765
Micrococcus sp.
-
Other publictions for EC 1.1.1.114
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285764
Chandrsekaran
-
D-Apiitol dehydrogenase from b ...
Micrococcus sp.
Methods Enzymol.
89
228-232
1982
-
-
-
-
-
-
1
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
4
-
-
1
1
-
-
-
1
-
-
1
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
285765
Hanna
-
Purification of a specific D-A ...
Micrococcus sp.
Biochim. Biophys. Acta
315
259-271
1973
-
-
-
-
-
-
-
4
-
-
1
-
-
1
-
-
1
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285766
Neal
D-Apiose reductase from Aeroba ...
Enterobacter aerogenes
J. Bacteriol.
101
910-915
1970
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-