Information on EC 1.1.1.114 - apiose 1-reductase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
1.1.1.114
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RECOMMENDED NAME
GeneOntology No.
apiose 1-reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-apiitol + NAD+ = D-apiose + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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SYSTEMATIC NAME
IUBMB Comments
D-apiitol:NAD+ 1-oxidoreductase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-apiitol reductase
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D-apiose reductase
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CAS REGISTRY NUMBER
COMMENTARY
37250-45-6
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gram-negativ coccus isolated from the surface of germinating parsley seeds by enrichment culture
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
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D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
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-
-
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D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
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r
D-erythrose + NADH
meso-erythritol + NAD+
show the reaction diagram
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1.5% activity of D-apiose reduction, 1% of D-apiitol oxidation
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r
D-mannitol + NAD+
D-mannose + NADH + H+
show the reaction diagram
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1% activity of D-apiitol oxidation
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?
D-ribulose + NADH
D-ribitol + NAD+
show the reaction diagram
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8.5% activity of D-apiose reduction, 5.5% of D-apiitol oxidation
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r
meso-erythritol + NAD+
D-erythrose + NADH
show the reaction diagram
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meso-erythritol + NAD+
D-erythrose + NADH
show the reaction diagram
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13% activity of D-apiitol oxidation
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ir
myo-inositol + NAD+
keto-inositol + NADH
show the reaction diagram
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myo-inositol + NAD+
keto-inositol + NADH
show the reaction diagram
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38% activity of D-apiitol oxidation
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
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r
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
10
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D-Apiitol
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enzyme activity in crude extracts
11.6
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D-Apiitol
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20
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D-apiose
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enzyme activity in crude extracts
71.4
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D-apiose
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
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reduction of D-apiose
7.5
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reduction of D-apiose, enzyme activity in crude extracts
10
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oxidation of D-apiitol
10.5
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oxidation of D-apiitol, enzyme activity in crude extracts
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
110000
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sucrose density centrifugation, gel filtration
115000
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sucrose density centrifugation, gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, several weeks, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
streptomycin sulfate, calcium phosphate gel, ethanol precipitation, DEAE-cellulose, Sephadex G-200
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