Information on EC 1.1.1.114 - apiose 1-reductase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
1.1.1.114
-
RECOMMENDED NAME
GeneOntology No.
apiose 1-reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-apiitol + NAD+ = D-apiose + NADH + H+
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
D-apiitol:NAD+ 1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-45-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
D-erythrose + NADH
meso-erythritol + NAD+
show the reaction diagram
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1.5% activity of D-apiose reduction, 1% of D-apiitol oxidation
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r
D-mannitol + NAD+
D-mannose + NADH + H+
show the reaction diagram
-
1% activity of D-apiitol oxidation
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?
D-ribulose + NADH
D-ribitol + NAD+
show the reaction diagram
-
8.5% activity of D-apiose reduction, 5.5% of D-apiitol oxidation
-
r
meso-erythritol + NAD+
D-erythrose + NADH
show the reaction diagram
myo-inositol + NAD+
keto-inositol + NADH
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-apiitol + NAD+
D-apiose + NADH
show the reaction diagram
-
-
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10 - 11.6
D-Apiitol
20 - 71.4
D-apiose
0.35
NAD+
-
-
0.015
NADH
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
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oxidation of D-apiitol
10.5
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oxidation of D-apiitol, enzyme activity in crude extracts
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
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sucrose density centrifugation, gel filtration
115000
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sucrose density centrifugation, gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, several weeks, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
streptomycin sulfate, calcium phosphate gel, ethanol precipitation, DEAE-cellulose, Sephadex G-200
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