Application | Comment | Organism |
---|---|---|
synthesis | synthesis of the cinnamyl alcohol by means of enzymatic reduction of cinnamaldehyde using alcohol both as an isolated enzyme, and in recombinant Escherichia coli whole cells in an efficient and sustainable one-phase system. The reduction of cinnamaldehyde (0.5 g/l, 3.8 mmol/l) by the isolated enzyme occurrs in 3 h at 50°C with 97% conversion, and yields high purity cinnamyl alcohol (98%) with a yield of 88% and a productivity of 50 g/g enzyme. The reduction of 12.5 g/l (94 mmol/l) cinnamaldehyde by whole cells in 6 h, at 37°C and no requirement of external cofactor occurrs with 97% conversion, 82% yield of 98% pure alcohol and a productivity of 34 mg/g wet cell weight | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADH | pH 7.7, 60°C | Geobacillus stearothermophilus | |
0.03 | - |
cinnamaldehyde | pH 7.7, 60°C | Geobacillus stearothermophilus | |
0.11 | - |
cinnamyl alcohol | pH 7.7, 60°C | Geobacillus stearothermophilus | |
0.45 | - |
NAD+ | pH 7.7, 60°C | Geobacillus stearothermophilus | |
57.8 | - |
2-propanol | pH 7.7, 60°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + NAD+ | - |
Geobacillus stearothermophilus | aceton + NADH + H+ | irreversible, no measurable activity with acetone | ir | |
cinnamaldehyde + NADH + H+ | - |
Geobacillus stearothermophilus | cinnamyl alcohol + NAD+ | - |
r | |
cinnamyl alcohol + NAD+ | - |
Geobacillus stearothermophilus | cinnamaldehyde + NADH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
NAD+ | pH 7.7, 60°C | Geobacillus stearothermophilus | |
43 | - |
NADH | pH 7.7, 60°C | Geobacillus stearothermophilus | |
43 | - |
cinnamyl alcohol | pH 7.7, 60°C | Geobacillus stearothermophilus | |
55 | - |
cinnamaldehyde | pH 7.7, 60°C | Geobacillus stearothermophilus | |
287 | - |
2-propanol | pH 7.7, 60°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
2-propanol | pH 7.7, 60°C | Geobacillus stearothermophilus | |
73 | - |
NAD+ | pH 7.7, 60°C | Geobacillus stearothermophilus | |
391 | - |
cinnamyl alcohol | pH 7.7, 60°C | Geobacillus stearothermophilus | |
1835 | - |
cinnamaldehyde | pH 7.7, 60°C | Geobacillus stearothermophilus | |
2687 | - |
NADH | pH 7.7, 60°C | Geobacillus stearothermophilus |