Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, complete inhibition | Magnusiomyces capitatus | |
2,2'-bipyridyl | 1 mM, 78% inhibition | Magnusiomyces capitatus | |
CoCl2 | 1 mM, 37% inhibition | Magnusiomyces capitatus | |
CuCl2 | 1 mM, complete inhibition | Magnusiomyces capitatus | |
FeCl3 | 0.1 mM, 13% inhibition | Magnusiomyces capitatus | |
HgCl2 | 1 mM, complete inhibition | Magnusiomyces capitatus | |
iodoacetic acid | 1 mM, 10% inhibition | Magnusiomyces capitatus | |
MgCl2 | 1 mM, 10% inhibition | Magnusiomyces capitatus | |
NiCl2 | 1 mM, complete inhibition | Magnusiomyces capitatus | |
PCMB | 0.1 mM, complete inhibition | Magnusiomyces capitatus | |
ZnSO4 | 1 mM, complete inhibition | Magnusiomyces capitatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
N-benzyl-3-pyrrolidinone | pH 6.0 | Magnusiomyces capitatus | |
0.79 | - |
NAD+ | pH 8.0 | Magnusiomyces capitatus | |
0.87 | - |
NADH | pH 6.0 | Magnusiomyces capitatus | |
8.47 | - |
(S)-N-benzyl-3-pyrrolidinol | pH 8.0 | Magnusiomyces capitatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Magnusiomyces capitatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Magnusiomyces capitatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
- |
Magnusiomyces capitatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + NAD+ | 35% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
1,2-hexanediol + NAD+ | 20% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
1,2-pentanediol + NAD+ | 12% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
1,3-butanediol + NAD+ | 39% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
1-hexanol + NAD+ | 15% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
1-hydroxy-2-butanone + NADH + H+ | 59% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | butane-1,2-diol + NAD+ | - |
? | |
1-pentanol + NAD+ | 8% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | pentanal + NADH + H+ | - |
? | |
1-phenyl-1-propanol + NAD+ | 31% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
2,3-butanediol + NAD+ | 83% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
2,3-butanedione + NADH + H+ | activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? | |
2,4-pentanediol + NAD+ | 21% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | ? | - |
? | |
2-butanol + NAD+ | activity is 3.7fold higher than with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | 2-butanone + NADH + H+ | - |
r | |
2-butanone + NADH + H+ | 40% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | 2-butanol + NAD+ | - |
r | |
2-hexanol + NAD+ | activity is 11.2fold higher than with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | 2-hexanone + NADH + H+ | - |
? | |
2-hexanone + NADH + H+ | activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? | |
2-pentanol + NAD+ | activity is 9fold higher than with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | 2-pentanone + NADH + H+ | - |
? | |
2-propanol + NAD+ | activity is 3.1fold higher than with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | 2-propanone + NADH + H+ | - |
? | |
3,4-hexanedione + NADH + H+ | 77% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? | |
acetophenone + NADH + H+ | 40% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | 1-phenylethanol + NAD+ | - |
? | |
acetylacetone + NADH + H+ | 63% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? | |
benzaldehyde + NADH + H+ | 74% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | benzyl alcohol + NAD+ | - |
? | |
benzylacetone + NADH + H+ | activity is 1.3fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | 4-phenylbutan-2-ol + NAD+ | - |
? | |
cyclohexanone + NADH + H+ | activity is 2.1fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? | |
hydroxyacetone + NADH + H+ | 27% of the activity with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | propane-1,2-diol + NAD+ | - |
? | |
additional information | no activity detected with: N-benzyl-2-pyrrolidinone, 2-pyrrolidinone, 3-hexanone, 4-hydroxy-2-butanone, (R)-N-benzyl-3-pyrrolidinol, ethanol, 1,3-propanediol, 1-butanol, 1,4-butanediol, 1,2,3-butanetriol, 1,2,4-butanetriol, acetol, 2-phenyl-1-propanol, 3-phenyl-1-propanol, benzyl alcohol and glycerol. No activity with NADP+ or NADPH | Magnusiomyces capitatus | ? | - |
? | |
N-benzyl-3-pyrrolidinol + NAD+ | 33% of the activity with (S)-N-benzyl-3-pyrrolidinol | Magnusiomyces capitatus | 1-benzylpyrrolidin-3-one + NADH + H+ | - |
? | |
N-benzyl-3-pyrrolidinone + NADH + H+ | (S)-N-benzyl-3-pyrrolidinol is produced at an enantiomeric excess of more than 99.9% | Magnusiomyces capitatus | (S)-N-benzyl-3-pyrrolidinol + NAD+ | - |
r | |
n-butylaldehyde + NADH + H+ | activity is 1.8fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | butanal + NAD+ | - |
? | |
n-hexylaldehyde + NADH + H+ | activity is 1.6fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | hexanal + NAD+ | - |
? | |
n-octylaldehyde + NADH + H+ | activity is 1.7fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | n-octanol + NAD+ | - |
? | |
n-valeraldehyde + NADH + H+ | activity is 2.8fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | n-pentanol + NAD+ | - |
? | |
propionaldehyde + NADH + H+ | activity is 1.1fold higher than with N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
reduction of N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 80% of maximal activity, 50°C: about 60% of maximal activity, reduction of N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | reduction of N-benzyl-3-pyrrolidinone | Magnusiomyces capitatus |