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Information on EC 6.5.1.B3 - 2'-5' RNA ligase (GTP-activated) and Organism(s) Pyrococcus furiosus and UniProt Accession Q8U4Q3

for references in articles please use BRENDA:EC6.5.1.B3
preliminary BRENDA-supplied EC number
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Pyrococcus furiosus
UNIPROT: Q8U4Q3
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The taxonomic range for the selected organisms is: Pyrococcus furiosus
The expected taxonomic range for this enzyme is: Pyrococcus
Reaction Schemes
a 3'-half-tRNA molecule with a 5'-oH end
+
a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
=
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
Synonyms
pf0027, ph0099, gtp-dependent rna ligase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GTP-dependent RNA ligase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
show the reaction diagram
-
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GTP
the activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of recombinant C-terminal His-tagged protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kanai, A.; Sato, A.; Fukuda, Y.; Okada, K.; Matsuda, T.; Sakamoto, T.; Muto, Y.; Yokoyama, S.; Kawai, G.; Tomita, M.
Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus
RNA
15
420-431
2009
Pyrococcus furiosus (Q8U4Q3), Pyrococcus furiosus
Manually annotated by BRENDA team