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Literature summary for 6.5.1.B3 extracted from

  • Kanai, A.; Sato, A.; Fukuda, Y.; Okada, K.; Matsuda, T.; Sakamoto, T.; Muto, Y.; Yokoyama, S.; Kawai, G.; Tomita, M.
    Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus (2009), RNA, 15, 420-431.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.B3

Activating Compound

Activating Compound Comment Organism Structure
GTP the activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of recombinant C-terminal His-tagged protein Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end Pyrococcus furiosus
-
 a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U4Q3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
-
 Pyrococcus furiosus a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
 ?
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro. The activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity. The enzyme cleaves a 2',3'-cyclic phosphate group to produce a 2'-phosphate group at the 3' terminus of an RNA oligomer Pyrococcus furiosus a mature tRNA molecule containing a 2'-5'-phosphodiester bond the ligation product is unstable during in vitro reaction ?

Synonyms

Synonyms Comment Organism
GTP-dependent RNA ligase
-
 Pyrococcus furiosus
PF0027
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Pyrococcus furiosus