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Information on EC 5.4.99.45 - tRNA pseudouridine38/39 synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P31115

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.45 tRNA pseudouridine38/39 synthase
IUBMB Comments
The enzyme from Saccharomyces cerevisiae is active only towards uridine38 and uridine39, and shows no activity with uridine40 (cf. EC 5.4.99.12, tRNA pseudouridine38-40 synthase) . In vitro the enzyme from mouse is active on uridine39 and very slightly on uridine38 (human tRNALeu) .
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Saccharomyces cerevisiae
UNIPROT: P31115
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
tRNA uridine38/39
=
tRNA pseudouridine38/39
Synonyms
Deg1, Deg1p, mPus3p, pseudouridine synthase 3, PUS3, PUS3/DEG1, Pus3p, more
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine38/39 uracil mutase
The enzyme from Saccharomyces cerevisiae is active only towards uridine38 and uridine39, and shows no activity with uridine40 (cf. EC 5.4.99.12, tRNA pseudouridine38-40 synthase) [1]. In vitro the enzyme from mouse is active on uridine39 and very slightly on uridine38 (human tRNALeu) [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine38
tRNA pseudouridine38
show the reaction diagram
tRNA uridine38/39
tRNA pseudouridine38/39
show the reaction diagram
-
-
-
?
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine38
tRNA pseudouridine38
show the reaction diagram
tRNA uridine38/39
tRNA pseudouridine38/39
show the reaction diagram
-
-
-
?
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
activity of yeast extract and purified yeast enzyme
37
activity of Escherichia coli extract
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D151A
inactive mutant
additional information
the thermosensitive growth phenotype of deg1 mutants is specifically suppressible by overexpression of tRNAGln UUG but not tRNAGln CUG or any other tRNA carrying U38 or U39, which is modified by Deg1 to Psi, with clear suppression of deg1-induced thermosensitivity by multicopy tRNAGln UUG but not tRNAGln CUG or other tester tRNAs. Rescue by multicopy tRNAGln UUG is clearly incomplete as growth at 39°C is not fully restored to wild-type levels. Negative genetic interactions between DEG1, Elongator and URM1 can be extended to conditions of TORC1 inhibition, rapamycin sensitivity of deg1, urm1 and elp3 single and all possible double mutant combinations, overview. Deletion of the DEG1 gene in strain W303-1B and score of adenine auxotrophy as well as color colony in direct comparison with an elp3 mutant that is known to affect SUP4-mediated read-through
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lecointe, F.; Simos, G.; Sauer, A.; Hurt, E.; Motorin, Y.; Grosjean, H.
Characterization of yeast protein Deg1 as pseudouridine synthase (pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop
J. Biol. Chem.
273
1316-1323
1998
Saccharomyces cerevisiae (P31115)
Manually annotated by BRENDA team
Lecointe, F.; Namy, O.; Hatin, I.; Simos, G.; Rousset, J-P.; Grosjean, H.
Lack of pseudouridine 38/39 in the anticodon arm of yeast cytoplasmic tRNA decreases in vivo recoding efficiency
J. Biol. Chem.
277
30445-30453
2002
Saccharomyces cerevisiae (P31115)
Manually annotated by BRENDA team
Hepfer, C.E.; Arnold-Croop, S.; Fogell, H.; Steudel, K.G.; Moon, M.; Roff, A.; Zaikoski, S.; Rickman, A.; Komsisky, K.; Harbaugh, D.L.; Lang, G.I.; Keil, R.L.
DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae
Mol. Genet. Genomics
274
528-538
2005
Saccharomyces cerevisiae (P31115)
Manually annotated by BRENDA team
Klassen, R.; Schaffrath, R.
Role of pseudouridine formation by Deg1 for functionality of two glutamine isoacceptor tRNAs
Biomolecules
7
pii: E8
2017
Saccharomyces cerevisiae (P31115), Saccharomyces cerevisiae ATCC 204508 / S288c / BY4741 (P31115)
Manually annotated by BRENDA team