Information on EC 5.4.99.32 - protostadienol synthase

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The expected taxonomic range for this enzyme is: Aspergillus fumigatus

EC NUMBER
COMMENTARY hide
5.4.99.32
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RECOMMENDED NAME
GeneOntology No.
protostadienol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3S)-2,3-epoxy-2,3-dihydrosqualene = (17Z)-protosta-17(20),24-dien-3beta-ol
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(S)-2,3-epoxysqualene mutase [cyclizing, (17Z)-protosta-17(20),24-dien-3beta-ol-forming]
(17Z)-Protosta-17(20),24-dien-3beta-ol is a precursor of the steroidal antibiotic helvolic acid.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S)-2,3-oxidosqualene
protosta-17(20)Z,24-dien-3beta-ol + (20R)-protosta-13(17),24-dien-3beta-ol
show the reaction diagram
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3:1 product ratio
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?
(3S)-oxidosqualene
(17Z)-protosta-17(20),24-dien-3beta-ol + (17Z)-protosta-13(17),24-dien-3beta-ol
show the reaction diagram
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
83090
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calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain GIL77
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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an OSPC mutant in which the C-terminal residues 702APPGGMR708 are replaced with 702NKSCAIS708, no longer produces the protostadienol, but instead efficiently produces a 1:1 mixture of lanosterol and parkeol (oxidosqualene:lanosterol cyclase activity)