Information on EC 5.3.3.6 - methylitaconate DELTA-isomerase

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The expected taxonomic range for this enzyme is: Eubacterium barkeri

EC NUMBER
COMMENTARY
5.3.3.6
-
RECOMMENDED NAME
GeneOntology No.
methylitaconate DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
methylitaconate = 2,3-dimethylmaleate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
isomerization
-, Q0QLE6
-
PATHWAY
KEGG Link
MetaCyc Link
Nicotinate and nicotinamide metabolism
-
nicotinate degradation III
-
SYSTEMATIC NAME
IUBMB Comments
methylitaconate DELTA2-DELTA3-isomerase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(R)-3-methylitaconate isomerase
-
-
3-methylitaconate-DELTA-isomerase
Q0QLE6
-
Isomerase, methylitaconate
-
-
-
-
Isomerase, methylitaconate DELTA-
-
-
-
-
MeIT isomerase
-
-
-
-
Methylitaconate isomerase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9059-08-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-, Q0QLE6
3-methylitaconate-DELTA-isomerase participates in the nicotinate fermentation pathway
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
itaconate
methylmaleate
show the reaction diagram
-, Q0QLE6
-
-
-
?
Methylitaconate
Dimethylmaleate
show the reaction diagram
-
-
-
-
?
Methylitaconate
Dimethylmaleate
show the reaction diagram
-
r
-
-
Methylitaconate
Dimethylmaleate
show the reaction diagram
-
r
-
-
Methylitaconate
Dimethylmaleate
show the reaction diagram
-
3-methylitaconate. The enzymatically active enantiomer has (R)-configuration
2,3-dimethylmaleate
-
Methylitaconate
Dimethylmaleate
show the reaction diagram
-
formation of dimethylmaleate is favored
-
-
Methylitaconate
?
show the reaction diagram
-
key step in the overall fermentation of nicotinate
-
-
-
methylitaconate
2,3-dimethylmaleate
show the reaction diagram
-, Q0QLE6
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Methylitaconate
?
show the reaction diagram
-
key step in the overall fermentation of nicotinate
-
-
-
methylitaconate
2,3-dimethylmaleate
show the reaction diagram
-, Q0QLE6
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no metal ion required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
iodoacetic acid
-
-
p-Chloromercuriphenyl sulfonic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
10 mM, 30% increase in activity
mercaptan
-
required for optimal activity
additional information
-
enzyme contains sulfhydryl groups
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4
-
dimethylmaleate
-
-
60
-
Itaconate
-, Q0QLE6
-
6.2
-
methylitaconate
-
-
7
-
methylitaconate
-, Q0QLE6
(R,S)-3-methylitaconate
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2
-
Itaconate
-, Q0QLE6
-
1720
-
methylitaconate
-, Q0QLE6
(R,S)-3-methylitaconate
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-, Q0QLE6
activity assay
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-, Q0QLE6
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
not detectable in cells grown on glucose
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40270
-
-, Q0QLE6
theoretical, without Strep-tag
41500
-
-, Q0QLE6
theoretical, including the Strep-tag
45000
-
-, Q0QLE6
apparent molecular mass, determined by SDS-PAGE
140000
-
-
sucrose density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homotetramer
-, Q0QLE6
4 * 40270 Da
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the crystal structure of 3-methylitaconate-DELTA-isomerase is solved at a resolution of 2.7 A
-, Q0QLE6
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, protein concentration 1 mg/ml, little loss of activity after 60 days
-
-80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months
-
0°C, protein concentration 1 mg/ml, little loss of activity after 6 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
affinity purification on a streptavidin column
-, Q0QLE6
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
into a pASG-IBA3 vector for expression in Escherichia coli Rosetta cells
-, Q0QLE6