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Literature summary for 5.3.3.6 extracted from
- Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase (1971), J. Biol. Chem., 246, 3378-3388.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 30% increase in activity | Eubacterium barkeri |  |
| mercaptan | required for optimal activity | Eubacterium barkeri | |
| additional information | enzyme contains sulfhydryl groups | Eubacterium barkeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GSSG | - |
Eubacterium barkeri | |
| iodoacetic acid | - |
Eubacterium barkeri | |
| p-chloromercuriphenyl sulfonic acid | - |
Eubacterium barkeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
dimethylmaleate | - |
Eubacterium barkeri | |
| 6.2 | - |
methylitaconate | - |
Eubacterium barkeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion required | Eubacterium barkeri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | - |
sucrose density gradient centrifugation | Eubacterium barkeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Eubacterium barkeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Eubacterium barkeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, protein concentration 1 mg/ml, little loss of activity after 60 days | Eubacterium barkeri |
| -80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months | Eubacterium barkeri |
| 0°C, protein concentration 1 mg/ml, little loss of activity after 6 days | Eubacterium barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Methylitaconate | r | Eubacterium barkeri | Dimethylmaleate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.2 | - |
Eubacterium barkeri |
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Release 2023.1 (January 2023)
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