Information on EC 5.3.3.10 - 5-carboxymethyl-2-hydroxymuconate DELTA-isomerase

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The expected taxonomic range for this enzyme is: Gammaproteobacteria

EC NUMBER
COMMENTARY
5.3.3.10
-
RECOMMENDED NAME
GeneOntology No.
5-carboxymethyl-2-hydroxymuconate DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
-
-
5-carboxymethyl-2-hydroxymuconate = 5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
4-hydroxyphenylacetate degradation
-
Microbial metabolism in diverse environments
-
Tyrosine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
5-carboxymethyl-2-hydroxymuconate DELTA2,DELTA4-2-oxo,DELTA3-isomerase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5-(Carboxymethyl)-2-hydroxymuconate isomerase
-
-
-
-
5-(Carboxymethyl)-2-hydroxymuconate isomerase
-
-
5-Carboxymethyl-2-hydroxymuconate isomerase
-
-
-
-
5-carboxymethyl-2-hydroxymuconic acid isomerase
-
-
-
-
CHM isomerase
-
-
-
-
CHMI
-
-
-
-
CMH isomerase
-
-
-
-
Isomerase, 5-carboxymethyl-2-hydroxymuconate
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
79079-05-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
-
-
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
-
-
-
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
-
-
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
-
-
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
-
-
R-configuration at C-5
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of homoprotocatechuate catabolic pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
reaction in homoprotocatechuate meta-fission pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of homoprotocatechuate catabolic pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
reaction in homoprotocatechuate meta-fission pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway
-
-
-
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
-
enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
enzyme is active in absence of Mg2+
-
additional information
-
enzyme is active in absence of Mg2+
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.28
-
5-Carboxymethyl-2-hydroxymuconate
-
-
-
0.3
-
5-Carboxymethyl-2-hydroxymuconate
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
122.1
-
-
-
148.3
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9
-
pH 6.5: about 30% of maximal activity, pH 7: about 85% of maximal activity, pH 9.0: about 45% of maximal activity
PDB
SCOP
CATH
ORGANISM
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
Mycobacterium marinum (strain ATCC BAA-535 / M)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30000
-
-
gel filtration
38000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 14000, SDS-PAGE; 2 * 14044, calculation from nucleotide sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, stable for at least 4 weeks
-
5°C, 0.01 M Na2HPO4-KH2PO4 buffer, pH 7.0, 1 mM dithiothreitol, stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE