Information on EC 5.3.3.10 - 5-carboxymethyl-2-hydroxymuconate DELTA-isomerase

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The expected taxonomic range for this enzyme is: Gammaproteobacteria

EC NUMBER
COMMENTARY hide
5.3.3.10
-
RECOMMENDED NAME
GeneOntology No.
5-carboxymethyl-2-hydroxymuconate DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-hydroxyphenylacetate degradation
Microbial metabolism in diverse environments
-
-
Tyrosine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
5-carboxymethyl-2-hydroxymuconate DELTA2,DELTA4-2-oxo,DELTA3-isomerase
Part of the homoprotocatechuate degradation pathway in Escherichia coli C.
CAS REGISTRY NUMBER
COMMENTARY hide
79079-05-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate
5-carboxy-2-oxohept-3-enedioate
show the reaction diagram
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-carboxymethyl-2-hydroxymuconate
?
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 0.3
5-Carboxymethyl-2-hydroxymuconate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
pH 6.5: about 30% of maximal activity, pH 7: about 85% of maximal activity, pH 9.0: about 45% of maximal activity
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
Mycobacterium marinum (strain ATCC BAA-535 / M)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
gel filtration
38000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 14000, SDS-PAGE; 2 * 14044, calculation from nucleotide sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for at least 4 weeks
-
5°C, 0.01 M Na2HPO4-KH2PO4 buffer, pH 7.0, 1 mM dithiothreitol, stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (1127 entries)
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