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Literature summary for 5.3.3.10 extracted from
- Isomerase and decarboxylase activities in the 4-hydroxyphenylacetate catabolic pathway of Pseudomonas putida (1985), Z. Naturforsch. C, 40, 503-508.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is active in absence of Mg2+ | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
5-Carboxymethyl-2-hydroxymuconate | - |
Pseudomonas putida |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-carboxymethyl-2-hydroxymuconate | Pseudomonas putida | enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 122.1 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 0.01 M Na2HPO4-KH2PO4 buffer, pH 7.0, 1 mM dithiothreitol, stable for at least 1 month | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-carboxymethyl-2-hydroxymuconate | - |
Pseudomonas putida | 5-carboxy-2-oxohept-3-enedioate | - |
? | |
| 5-carboxymethyl-2-hydroxymuconate | enzyme of the 4-hydroxyphenylacetate meta-cleavage pathway | Pseudomonas putida | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 30% of maximal activity, pH 7: about 85% of maximal activity, pH 9.0: about 45% of maximal activity | Pseudomonas putida |
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