Information on EC 4.3.1.4 - formimidoyltetrahydrofolate cyclodeaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
4.3.1.4
-
RECOMMENDED NAME
GeneOntology No.
formimidoyltetrahydrofolate cyclodeaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-formimidoyltetrahydrofolate = 5,10-methenyltetrahydrofolate + NH3
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-histidine degradation III
-
-
Metabolic pathways
-
-
One carbon pool by folate
-
-
purine nucleobases degradation I (anaerobic)
-
-
purine nucleobases degradation II (anaerobic)
-
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histidine metabolism
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
5-formimidoyltetrahydrofolate ammonia-lyase (cyclizing; 5,10-methenyltetrahydrofolate-forming)
In eukaroytes, occurs as a bifunctional enzyme that also has glutamate formimidoyltransferase (EC 2.1.2.5) activity.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-05-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Madin-Darby
-
-
Manually annotated by BRENDA team
Madin-Darby
-
-
Manually annotated by BRENDA team
Clostridium acidi-urici
-
-
-
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-formiminotetrahydrofolate
?
show the reaction diagram
-
-
-
-
?
(-)-formiminotetrahydropteroyl-gamma-L-glutamyl-gamma-L-glutamyl-L-glutamate
?
show the reaction diagram
-
-
-
-
?
(-)-formiminotetrahydropteroyl-L-aspartate
?
show the reaction diagram
-
-
-
-
?
(-/+)-formiminotetrahydropteroate
?
show the reaction diagram
-
-
-
-
?
(-/+)-formiminotetrahydropteroyl-D-glutamate
?
show the reaction diagram
-
-
-
-
?
(6S)-5-formimino-5,6,7,8-tetrahydropteroylglutamate
?
show the reaction diagram
(6S)-5-formimino-5,6,7,8-tetrahydropteroylheptaglutamate
?
show the reaction diagram
-
-
-
-
?
(6S)-5-formimino-5,6,7,8-tetrahydropteroylhexaglutamate
?
show the reaction diagram
-
-
-
-
?
(6S)-5-formimino-5,6,7,8-tetrahydropteroylpentaglutamate
?
show the reaction diagram
(6S)-5-formimino-5,6,7,8-tetrahydropteroyltetraglutamate
?
show the reaction diagram
(6S)-5-formimino-5,6,7,8-tetrahydropteroyltriglutamate
?
show the reaction diagram
5-formimidoyltetrahydrofolate
5,10-methenyltetrahydrofolate + NH3
show the reaction diagram
5-formiminotetrahydrofolate
5,10-methenyltetrahydrofolate + NH3
show the reaction diagram
5-formiminotetrahydrofolate
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-formimidoyltetrahydrofolate
5,10-methenyltetrahydrofolate + NH3
show the reaction diagram
5-formiminotetrahydrofolate
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(6S)-5,6,7,8-tetrahydropteroylpolyglutamate
-
-
10-methyltetrahydrofolate
-
competitive
2-hydroxy-2-deaminotetrahydrofolate
-
competitive
4-amino-4-deoxy-10-methylfolate
-
competitive
4-amino-4-deoxyfolate
-
competitive
4-amino-4-deoxytetrahydrofolate
-
competitive
5,5'-dithiobis(2-nitrobenzoate)
folate
-
-
HgCl2
-
0.05 mM, triethanolamine-HCl, pH 8.0, 95% loss of activity
tetrahydrofolate
Urea
-
complete inhibition at a concentration of 1 M in triethanolamine hdrochloride buffer, folate and pteroylpolyglutamates protect
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.031
(-)-formiminotetrahydrofolate
-
-
0.04
(-)-formiminotetrahydropteroyl-L-aspartate
-
-
0.029
(-)-formiminotetrahydropteroyltri-L-glutamate
-
-
0.149
(6S)-5-formimino-5,6,7,8-tetrahydropteroylglutamate
-
-
0.0021
(6S)-5-formimino-5,6,7,8-tetrahydropteroylheptaglutamate
-
-
0.0012
(6S)-5-formimino-5,6,7,8-tetrahydropteroylhexaglutamate
-
-
0.002
(6S)-5-formimino-5,6,7,8-tetrahydropteroylpentaglutamate
-
-
0.0029
(6S)-5-formimino-5,6,7,8-tetrahydropteroyltetraglutamate
-
-
0.021
(6S)-5-formimino-5,6,7,8-tetrahydropteroyltriglutamate
-
-
0.079
(6S)-5-formiminotetrahydropteroylglutamate
-
monofunctional dimer
0.017
(6S)-5-formiminotetrahydropteroylpentaglutamate
-
monofunctional dimer
0.026 - 0.175
5-formiminotetrahydrofolate
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
pH 5: 20% of maximal activity, pH 8: 30% of maximal activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 45
-
enzyme activity increases in a linear manner, temperature optimum is not determined
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
hepatocellular carcinoma tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
no interaction aith liver-specific cytoskeleton proteins
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
calculated from Svedberg equation (sedimentation equilibrium?)
58000
-
8 * 58000, SDS-PAGE
58870
-
predicted and determined by immunoblot analysis of protein lysates
58926
-
8 * 58926, calculation from sequence of amino acid, formiminotransferase-cyclodeaminase enzyme complex
59100
8 * 59100, calculation from sequence of amono acid, isoform of formiminotransferase-cyclodeaminase enzyme complex
65800
-
8 * 65800, sedimentation equilibrium in 5 M guanidine HCl, formiminotransferase-cyclodeaminase enzyme complex
496000
-
sedimentation analysis
500000
-
octamer
538000
-
sedimentation equilibrium, formiminotransferase-cyclodeaminase enzyme complex
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homooctamer
octamer
oligomer
-
present in cells as an oligomer with a MG of 240000, each subunit has a MG of 62000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals belong to space group P4 with unit cell dimensions of a = b = 134.85 A and c = 156.37 A and four subunits in the asymmetric unit for a total of 32 selenium atoms
formiminotransferase-cyclodeaminase enzyme complex
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in presence of folinic acid, two subdomains in a novel alpha/beta fold
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nanodroplet vapor diffusion method, space group P2(1)2(1)2(1), unit cell parameters: a = 56.7 A, b = 71.88 A, c = 119.25 A
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.8
-
stable for 2 h at 0°C
34312
additional information
-
-
34312
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several months at concentrations of 1 mg/ml
-
enzyme loses activity after repeated freezing and thawing
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
formiminotransferase-cyclodeaminase enzyme complex
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partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli, formiminotransferase-cyclodeaminase enzyme complex
-
formiminotransferase-cyclodeaminase enzyme complex
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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formiminotransferase cyclodeaminase enzyme complex represents a marker with which to explore ER-Golgi dynamics
diagnostics
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putative biomarker, FTCD is useful to distinguish early hepatocellular carcinoma from benign tumors such as regenerative nodule or focal nodular hyperplasia
medicine
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