Information on EC 4.2.1.81 - D(-)-tartrate dehydratase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
4.2.1.81
-
RECOMMENDED NAME
GeneOntology No.
D(-)-tartrate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S,S)-tartrate = oxaloacetate + H2O
show the reaction diagram
requires Fe2+; requires Fe2+ or Mn2+. cf. EC 4.2.1.32 L(+)-tartrate dehydratase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C-O bond cleavage by elimination of water
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(S,S)-tartrate hydro-lyase (oxaloacetate-forming)
Requires Fe2+ or Mn2+. cf. EC 4.2.1.32 L(+)-tartrate dehydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
82532-88-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains G2 and Ch
-
-
Manually annotated by BRENDA team
Si4
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S,S)-tartrate
oxaloacetate + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S,S)-tartrate
oxaloacetate + H2O
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 55% (strain Ch) efficiency
Co2+
-
requirement, 0.5 mM, equally effective as Fe2+
Divalent cations
Fe2+
-
requirement, 0.1 mM, equally effective as Co2+
Mg2+
-
requirement, can replace Fe2+ or Co2+ with about 60% (strain G2) and 80% (strain Ch) efficiency
Mn2+
-
requirement, can replace Fe2+ or Co2+ with about 65% efficiency
Ni2+
-
requirement, can replace Fe2+ or Co2+ with about 30% (strain G2) and 80% (strain Ch) efficiency
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-mercaptoethanol
-
-
D-malate
dithioerythritol
-
-
glycolate
-
weak
L(+)-Tartrate
L-Malate
meso-tartrate
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035 - 7.7
(S,S)-Tartrate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0019 - 7.3
(S,S)-Tartrate
19.8
D-Tartrate
Rhodobacter sphaeroides
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.1
-
strain Ch
13.6
-
strain G2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
highest activity in Tris-HCl buffer, less effective are N-2'-hydroxyethylpiperazine-2-ethanesulfonic acid, glycylglycine, triethanolamine-HCl, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, imidazole or potassium phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
-
strain Ch, gel filtration
78000
-
strain G2, gel filtration
158000
-
gel filtration, ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 38500, SDS-PAGE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
all crystals, wild type TarD liganded with Mg2+, selenomethionine-substituted TarD liganded with Mg2+, wild-type TarD liganded with Mg2+ and meso-tartrate, and the K184A mutant liganded with Mg2+ and D-tartrate, are grown by the hanging drop method at room temperature
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
-
5 min, 50% loss of activity at 30C
648403
6 - 8.7
-
stable at least 5 min at 30C
648403
9.4
-
5 min, 50% loss of activity at 30C
648403
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable at least 5 min at pH 6-8.7, 50% loss of activity at pH 4.9 and pH 9.4 after 5 min
45
-
30 min, 50% loss of activity
50
-
3 min, 50% loss of activity
55
-
1 min, 50% loss of activity
60
-
12 s, 50% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
omission of Mg2+ from standard buffer results in rapid inactivation during purification with irreversible loss of activity after ammonium sulfate precipitation, Mg2+, Mn2+, Zn2+, Ca2+ or Co2+ stabilizes, not Ni2+
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
insensitive to O2, no thiols required for protection, 15 h stable at 22C
-
648404
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 1.5 mg enzyme/ml 0.1 M Tris-HCl buffer, pH 7.2, 1 mM MgCl2, 20% loss of activity within 5 months
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22C, 50% loss of activity within 10 days
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4C, 50% loss of activity within 6 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to near homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain BL21
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H322A
-
kcatKm is 193000fold lower than wild-type value
H322N
-
kcatKm is 607fold lower than wild-type value
H322Q
-
kcatKm is 340000fold lower than wild-type value
K102A
-
inactive mutant protein
K102M
-
inactive mutant protein
K184A
-
inactive mutant enzyme
K184R
-
reduced activity. kcat/Km is 500fold lower than wild-type value
additional information
-
selection of strains for constitutive production of enzyme for selective cleavage of racemic tartaric acid and quantitative detection of D(-)-tartrate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
constitutive production of enzyme for selective cleavage of racemic tartaric acid and quantitative detection of D(-)-tartrate