Crystallization (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
General Stability | Organism |
---|---|
omission of Mg2+ from standard buffer results in rapid inactivation during purification with irreversible loss of activity after ammonium sulfate precipitation, Mg2+, Mn2+, Zn2+, Ca2+ or Co2+ stabilizes, not Ni2+ | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | - |
Cereibacter sphaeroides | |
citrate | weak | Cereibacter sphaeroides | |
Cu2+ | - |
Cereibacter sphaeroides | |
D-malate | weak | Cereibacter sphaeroides | |
dithioerythritol | - |
Cereibacter sphaeroides | |
EDTA | strong | Cereibacter sphaeroides | |
Fe2+ | - |
Cereibacter sphaeroides | |
glycolate | weak | Cereibacter sphaeroides | |
L(+)-Tartrate | weak | Cereibacter sphaeroides | |
L-malate | weak | Cereibacter sphaeroides | |
meso-tartrate | - |
Cereibacter sphaeroides | |
additional information | no inhibition by iodoacetamide, N-ethylmaleimide; no inhibition by iodoacetate | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
(S,S)-Tartrate | - |
Cereibacter sphaeroides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cereibacter sphaeroides | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | not | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39500 | - |
4 * 39500, SDS-PAGE | Cereibacter sphaeroides |
158000 | - |
gel filtration, ultracentrifugation | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S,S)-tartrate | Cereibacter sphaeroides | - |
oxaloacetate + H2O | - |
ir | |
(S,S)-tartrate | Cereibacter sphaeroides Si4 / DSM 8371 | - |
oxaloacetate + H2O | - |
ir | |
additional information | Cereibacter sphaeroides | involved in D-tartrate catabolism | ? | - |
? | |
additional information | Cereibacter sphaeroides Si4 / DSM 8371 | involved in D-tartrate catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Cereibacter sphaeroides Si4 / DSM 8371 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-20°C, 1.5 mg enzyme/ml 0.1 M Tris-HCl buffer, pH 7.2, 1 mM MgCl2, 20% loss of activity within 5 months | Cereibacter sphaeroides |
22°C, 50% loss of activity within 10 days | Cereibacter sphaeroides |
4°C, 50% loss of activity within 6 weeks | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S,S)-tartrate | - |
Cereibacter sphaeroides | oxaloacetate + H2O | - |
ir | |
(S,S)-tartrate | no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol | Cereibacter sphaeroides | oxaloacetate + H2O | at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate | ir | |
(S,S)-tartrate | - |
Cereibacter sphaeroides Si4 / DSM 8371 | oxaloacetate + H2O | - |
ir | |
(S,S)-tartrate | no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol | Cereibacter sphaeroides Si4 / DSM 8371 | oxaloacetate + H2O | at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate | ir | |
additional information | involved in D-tartrate catabolism | Cereibacter sphaeroides | ? | - |
? | |
additional information | involved in D-tartrate catabolism | Cereibacter sphaeroides Si4 / DSM 8371 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39500, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
48 | - |
- |
Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable at least 5 min at pH 6-8.7, 50% loss of activity at pH 4.9 and pH 9.4 after 5 min | Cereibacter sphaeroides |
45 | - |
30 min, 50% loss of activity | Cereibacter sphaeroides |
50 | - |
3 min, 50% loss of activity | Cereibacter sphaeroides |
55 | - |
1 min, 50% loss of activity | Cereibacter sphaeroides |
60 | - |
12 s, 50% loss of activity | Cereibacter sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.8 | - |
D-Tartrate | - |
Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
highest activity in Tris-HCl buffer, less effective are N-2'-hydroxyethylpiperazine-2-ethanesulfonic acid, glycylglycine, triethanolamine-HCl, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, imidazole or potassium phosphate buffer | Cereibacter sphaeroides |
6.2 | 7.2 | - |
Cereibacter sphaeroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.9 | - |
5 min, 50% loss of activity at 30°C | Cereibacter sphaeroides |
6 | 8.7 | stable at least 5 min at 30°C | Cereibacter sphaeroides |
9.4 | - |
5 min, 50% loss of activity at 30°C | Cereibacter sphaeroides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cereibacter sphaeroides | isoelectric focusing | - |
5.5 |