Information on EC 4.2.1.155 - (methylthio)acryloyl-CoA hydratase

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The expected taxonomic range for this enzyme is: Ruegeria pomeroyi

EC NUMBER
COMMENTARY hide
4.2.1.155
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RECOMMENDED NAME
GeneOntology No.
(methylthio)acryloyl-CoA hydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-(methylthio)acryloyl-CoA + 2 H2O = acetaldehyde + methanethiol + CoA + CO2
show the reaction diagram
3-(methylthio)acryloyl-CoA + H2O = 3-hydroxy-3-(methylthio)propanoyl-CoA
show the reaction diagram
3-hydroxy-3-(methylthio)propanoyl-CoA = 3-oxopropanoyl-CoA + methanethiol
show the reaction diagram
3-oxopropanoate = acetaldehyde + CO2
show the reaction diagram
3-oxopropanoyl-CoA + H2O = 3-oxopropanoate + CoA
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methylthiopropanoate degradation I (cleavage)
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Sulfur metabolism
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SYSTEMATIC NAME
IUBMB Comments
3-(methylsulfanyl)prop-2-enoyl-CoA hydro-lyase (acetaldehyde-forming)
The enzyme is involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton. Isolated from the bacterium Ruegeria pomeroyi.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(methylthio)acryloyl-CoA + 2 H2O
acetaldehyde + methanethiol + CoA + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-(methylthio)acryloyl-CoA + 2 H2O
acetaldehyde + methanethiol + CoA + CO2
show the reaction diagram
PDB
SCOP
CATH
ORGANISM
UNIPROT
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystals of the wild-type enzyme and the E121A mutant enzyme are grown at 20°C by the hanging-drop vapor diffusion method. Crystal structure of the free enzyme at 1.5 A resolution, structures of the E121A mutant in complex with methylthioacryloyl-CoA and 3-methylmercaptopropionate-CoA at 1.8 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the recombinant protein, with a C-terminal hexahistidine tag, was over-expressed in E. coli BL21 (DE3) Star cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE