Information on EC 4.2.1.133 - copal-8-ol diphosphate hydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.133
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RECOMMENDED NAME
GeneOntology No.
copal-8-ol diphosphate hydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate = geranylgeranyl diphosphate + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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labdane-type diterpenes biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranylgeranyl-diphosphate hydro-lyase [(13E)-8alpha-hydroxylabda-13-en-15-yl diphosphate forming]
Requires Mg2+. The enzyme was characterized from the plant Cistus creticus subsp. creticus.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subsp. creticus, gene CcCLS
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
encodes a gene CPS2 orthologue, encoding for copal-8-ol diphosphate synthase, CLS, in other Solanum sp., but which here encodes a nonfunctional protein
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Manually annotated by BRENDA team
encodes a gene CPS2 orthologue, encoding for copal-8-ol diphosphate synthase, CLS, in other Solanum sp., but which here encodes a nonfunctional protein
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
copal-8-ol diphosphate, which is made from the all-trans geranylgeranyl diphosphate by copal-8-ol diphosphate synthase Nt-CPS2, is the substrate for Z-abienol synthase
physiological function
the enzyme is responsible for synthesis of copal-8-ol diphosphate, which is likely to be an intermediate in the biosynthesis of the oxygen-containing labdane-type diterpenes that are abundant in the resin of this plant. CLS is a putative key enzyme in the biosynthesis of oxygen-containing labdane-type diterpenes
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + H2O
(13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate
show the reaction diagram
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i.e. copal-8-ol diphosphate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
of young leaves
Manually annotated by BRENDA team
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant CcCLS from Escherichia coli by affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene CcCLS, DNA and amino acid sequence determination, analysis, and comparisons, cloning from cDNA library, expression in Escherichia coli
gene CPS2, located in a gene cluster on chromosome 8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic organization
gene Nt-CPS2, located in a gene cluster on chromosome 8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic organization
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is increased by wounding of leaves