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Literature summary for 4.2.1.133 extracted from
- A copal-8-ol diphosphate synthase from the angiosperm Cistus creticus subsp. creticus is a putative key enzyme for the formation of pharmacologically active, oxygen-containing labdane-type diterpenes (2010), Plant Physiol., 154, 301-310.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CcCLS, DNA and amino acid sequence determination, analysis, and comparisons, cloning from cDNA library, expression in Escherichia coli | Cistus creticus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cistus creticus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Cistus creticus | CcCLS catalyzes the formation of stable bicyclic intermediate copal-8-ol diphosphate from geranylgeranyl diphosphate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cistus creticus | E2IHE0 | subsp. creticus, gene CcCLS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CcCLS from Escherichia coli by affinity chromatography | Cistus creticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | young | Cistus creticus | - |
| additional information | CcCLS is preferentially expressed in the trichomes, with higher transcript levels found in glands on young leaves than on fully expanded leaves, while CcCLS transcript levels increase after mechanical wounding | Cistus creticus | - |
| trichome | of young leaves | Cistus creticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | CcCLS catalyzes the formation of stable bicyclic intermediate copal-8-ol diphosphate from geranylgeranyl diphosphate | Cistus creticus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CcCLS | - |
Cistus creticus |
| copal-8-ol diphosphate synthase | - |
Cistus creticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cistus creticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cistus creticus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cistus creticus | enzyme expression is increased by wounding of leaves | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | protein-based phylogenetic analysis of plant diterpene synthases, overview | Cistus creticus |
| physiological function | the enzyme is responsible for synthesis of copal-8-ol diphosphate, which is likely to be an intermediate in the biosynthesis of the oxygen-containing labdane-type diterpenes that are abundant in the resin of this plant. CLS is a putative key enzyme in the biosynthesis of oxygen-containing labdane-type diterpenes | Cistus creticus |
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Release 2023.1 (January 2023)
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