Information on EC 4.2.1.12 - phosphogluconate dehydratase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
4.2.1.12
-
RECOMMENDED NAME
GeneOntology No.
phosphogluconate dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-phospho-D-gluconate = 2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Entner Doudoroff pathway
-
-
Entner-Doudoroff pathway I
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Metabolic pathways
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Microbial metabolism in diverse environments
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Pentose phosphate pathway
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-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-D-gluconate hydro-lyase (2-dehydro-3-deoxy-6-phospho-D-gluconate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9024-33-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PW2, strain Lhet2, strain KLB, strain OP, strain QBP
-
-
Manually annotated by BRENDA team
strain CC409
-
-
Manually annotated by BRENDA team
strain CC409
-
-
Manually annotated by BRENDA team
strain AM511
-
-
Manually annotated by BRENDA team
strain AM511
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Methylophilus methanolovorus
-
-
-
Manually annotated by BRENDA team
strain Y1, strain Y2, strain Y6, strain S91, strain S85, strain M132
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-
Manually annotated by BRENDA team
Protaminobacter candidus
-
-
-
Manually annotated by BRENDA team
strain O6, rhizobacterium, wild-type and mutants of the edd gene encoding 6-phosphogluconate dehydratase
SwissProt
Manually annotated by BRENDA team
strain O6, rhizobacterium, wild-type and mutants of the edd gene encoding 6-phosphogluconate dehydratase
SwissProt
Manually annotated by BRENDA team
A3.12
-
-
Manually annotated by BRENDA team
Pseudomonas mildenbergii
-
-
-
Manually annotated by BRENDA team
Rhizobium lupini
strain RL3001
-
-
Manually annotated by BRENDA team
Rhizobium lupini RL3001
strain RL3001
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Rhizobium sp. BICC609
strain BICC609 (Cassia sophhera)
-
-
Manually annotated by BRENDA team
Rhizobium sp. BICC610
strain BICC610 (Sesbania bispinosa)
-
-
Manually annotated by BRENDA team
Rhizobium sp. BICC613
strain BICC613 (Tephrosia purpurea)
-
-
Manually annotated by BRENDA team
Rhizobium sp. BICC614
strain BICC614 (Clitoria ternatea)
-
-
Manually annotated by BRENDA team
strain U8 (Vigna radiata var. Mungo)
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain KACC10859
-
-
Manually annotated by BRENDA team
strain KACC10859
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-phospho-D-gluconate
2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O
show the reaction diagram
6-phospho-D-gluconate
6-phospho-2-dehydro-3-deoxy-D-gluconate + H2O
show the reaction diagram
6-phospho-D-gluconate
?
show the reaction diagram
additional information
?
-
-
multicopy plasmid suppression of stationary phase chaperone toxicity in E. coli by phosphogluconate dehydratase and the N-terminus of DnaK
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-phospho-D-gluconate
2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O
show the reaction diagram
6-phospho-D-gluconate
6-phospho-2-dehydro-3-deoxy-D-gluconate + H2O
show the reaction diagram
6-phospho-D-gluconate
?
show the reaction diagram
additional information
?
-
-
multicopy plasmid suppression of stationary phase chaperone toxicity in E. coli by phosphogluconate dehydratase and the N-terminus of DnaK
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
[4Fe-4S]-center
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-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
completely inactive in absence of divalent metal ion, Fe2+ is most effective
Mg2+
-
completely inactive in absence of divalent metal ion, 45% of the activation by Fe2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerophosphates
-
most strongly inhibited by the D-alpha-isomer which structurally corresponds to half of the substrate molecule
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H2O2
-
the enzyme is much less sensitive to O2 or H2O2 than to O2-
O2
-
the enzyme is much less sensitive to O2 or H2O2 than to O2-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04 - 0.6
6-phosphogluconate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
coupled reaction of EC 4.2.1.12 plus EC 4.1.2.14
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Shewanella oneidensis (strain MR-1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 66000, SDS-PAGE
dimer
-
2 * 63000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
alpha-glycerophosphate stabilizes
-
Mn2+ stabilizes
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
F- stabilizes and blocks the efect of oxidants
-
33658
rapid inactivation under oxidizing conditions
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33650
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
one-step procedure
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, mutagenesis of the edd gene of Pseudomonas chlororaphis O6 encoding 6-phosphogluconate dehydratase, influence of different carbon sources on its expression and on bacterial growth analyzed
expression in Pseudomonmas aeruginosa and Pseudomonas putida
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
synthesis of 6-phospho-2-dehydro-3-deoxy-D-gluconate
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