Information on EC 4.2.1.106 - bile-acid 7alpha-dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.106
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RECOMMENDED NAME
GeneOntology No.
bile-acid 7alpha-dehydratase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
7alpha,12alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acids degradation
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Secondary bile acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
7alpha,12alpha-dihydroxy-3-oxochol-4-enoyl-CoA hydro-lyase (12alpha-hydroxy-3-oxochola-4,6-dienoyl-CoA-forming)
This enzyme, characterized from the gut bacterium Clostridium scindens (previously known as Eubacterium sp. strain VPI 12708), participates in the 7-dehydroxylation process associated with bile acid degradation.
CAS REGISTRY NUMBER
COMMENTARY hide
85130-33-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
strain TO-931
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Manually annotated by BRENDA team
strain TO-931
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Manually annotated by BRENDA team
strain VPI 12708
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Manually annotated by BRENDA team
strain VPI 12708
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
show the reaction diagram
7alpha-hydroxy-3-oxochol-4-en-24-oate
3-oxochola-4,6-dien-24-oate
show the reaction diagram
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
show the reaction diagram
7alpha-hydroxy-3-oxochol-4-enoate
3-oxocholate-4,6-dienoate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
12alpha-hydroxy-3-oxocholate-4,6-dienoate
show the reaction diagram
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
3-oxochola-4,6-dien-24-oyl-CoA
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16
7alpha,12alpha-dihydroxy-3-oxochol-4-enoate
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pH 7.5
9.9 - 25
7alpha-hydroxy-3-oxochol-4-en-24-oate
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1.2 - 208
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.4 - 24
7alpha-hydroxy-3-oxochol-4-en-24-oate
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12.6 - 98
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 2.4
7alpha-hydroxy-3-oxochol-4-en-24-oate
206968
0.1 - 24.5
7alpha-hydroxy-3-oxochol-4-en-24-oyl-CoA
206093
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19500
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2 * 19500, deduced from nucleotide sequence
23000
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2 * 23000, SDS-PAGE
36000
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gel filtration
114000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DE-52, Phenyl-Sepharose, HPLC-DEAE, gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of bai operon containing BaiE gene coding for bile acid 7alpha-dehydratase
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expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D106N
inactive mutant
D35N
inactive mutant
H83N
inactive mutant
R146Q
inactive mutant
Y126F
strongly reduced activity compared to wild type
Y30F
inactive mutant
Y54A
inactive mutant
Y54F
reduced activity compared to wild type
D106N
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inactive mutant
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D35N
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inactive mutant
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Y30F
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inactive mutant
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Y54A
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inactive mutant
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Y54F
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reduced activity compared to wild type
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