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EC Tree
IUBMB CommentsThe isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate .
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
icl, isocitrate lyase, citrate lyase, mtbicl, eggce, isocitritase, isocitratase, isocitric lyase, fpicl1, petal death protein,
more
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lyase, isocitrate
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-
-
-
threo-DS-Isocitrate glyoxylate-lyase
-
-
-
-
ICL
-
-
-
-
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isocitrate glyoxylate-lyase (succinate-forming)
The isomer of isocitrate involved is (1R,2S)-1-hydroxypropane-1,2,3-tricarboxylate [3].
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isocitrate
glyoxylate + succinate
-
-
-
?
(2R,3S)-isocitrate
succinate + glyoxylate
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-
-
-
?
DL-isocitrate
succinate + glyoxylate
-
-
-
-
?
isocitrate
?
-
glyoxylate cycle
-
-
?
isocitrate
succinate + glyoxylate
additional information
?
-
-
Lys-193, Cys-195, His-197 and His-356 are catalytic, active-site residues, while His-184 is involved in the assembly of the tetrameric enzyme
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-
?
isocitrate
succinate + glyoxylate
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-
-
?
isocitrate
succinate + glyoxylate
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-
-
?
isocitrate
succinate + glyoxylate
-
-
-
-
?
isocitrate
succinate + glyoxylate
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-
-
r
isocitrate
succinate + glyoxylate
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-
-
r
isocitrate
succinate + glyoxylate
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-
-
r
isocitrate
succinate + glyoxylate
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-
-
r
isocitrate
succinate + glyoxylate
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first step in glyoxylate-bypass pathway
-
?
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isocitrate
?
-
glyoxylate cycle
-
-
?
isocitrate
succinate + glyoxylate
isocitrate
succinate + glyoxylate
-
-
-
-
?
isocitrate
succinate + glyoxylate
-
first step in glyoxylate-bypass pathway
-
?
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Mg2+
-
no activity in absence of exogenous cation, Mg2+ most effective
Ni2+
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no activity in absence of exogenous cation, Ni2+ 7% as effective as Mg2+
Sr2+
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no activity in absence of exogenous cation, Sr2+ 3% as effective as Mg2+
Co2+
-
no activity in absence of exogenous cation
Co2+
-
Co2+ 17% as effective as Mg2+
Mn2+
-
no activity in absence of exogenous cation
Mn2+
-
Mn2+ 54% as effective as Mg2+
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3-nitropropionate
-
50% inhibition at 0.04 mM
glyoxylate
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linear competitive inhibitor
HgCl2
-
100% inhibition at 0.01 mM
Hydroxymalonate
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50% inhibition at 0.5 mM
methylmalonate
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50% inhibition at 15 mM
glycolate
-
-
glycolate
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50% inhibition at 5 mM
phosphoenolpyruvate
-
-
phosphoenolpyruvate
-
uncompetitive inhibitor
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0.015 - 0.096
DL-isocitrate
0.018 - 0.062
Ds-isocitrate
0.008
threo-Ds-isocitrate
-
-
0.25
isocitrate
10°C
0.015
DL-isocitrate
-
-
0.087 - 0.096
DL-isocitrate
-
-
0.018
Ds-isocitrate
-
pH 6.8
0.062
Ds-isocitrate
-
pH 7.3
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0.04
3-nitropropionate
-
-
0.893
phosphoenolpyruvate
-
in 100 mM potassium phosphate buffer (pH 7.1), 10 mM MgCl2, 12 mM cysteine
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0.022
-
strain BW3709, 0.2% glucose (batch)
0.023
-
strain BW3767, 0.2% glucose (batch)
0.034
-
strain BW2952, 0.2% glucose (batch)
0.088
-
strain BW3480, 0.02% glucose (chemostat)
0.155
-
strain MG1655, 0.2% glucose (batch)
0.19
-
strain BW3708, 0.2% glucose (batch)
0.248
-
strain BW2952, 0.02% glucose (chemostat)
0.471
-
strain BW3767, 0.02% glucose (chemostat)
0.538
-
strain BW3709, 0.02% glucose (chemostat)
1.066
-
strain BW2952, 0.2% acetate (batch)
1.227
-
strain BW3767, 0.2% acetate (batch)
1.656
-
strain BW3709, 0.2% acetate (batch)
1.674
-
strain MG1655, 0.02% glucose (chemostat)
1.778
-
strain BW3708, 0.02% glucose (chemostat)
2.084
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strain MG1655, 0.2% acetate (batch)
3.075
-
strain BW3708, 0.2% acetate (batch)
0.004
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strain BW3782, 0.02% glucose (chemostat)
0.004
-
strain BW3782, 0.2% glucose (batch)
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additional information
optimum temperature above 40°C
additional information
-
optimum temperature above 40°C
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-
Uniprot
brenda
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-
0.2% acetate (batch)
brenda
-
0.02% glucose (chemostat)
brenda
-
0.2% glucose (batch)
brenda
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-
-
brenda
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180000
-
sedimentation equilibrium centrifugation
44700
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4 * 44700, SDS-PAGE
48000
-
4 * 48000, SDS-PAGE
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tetramer
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-
tetramer
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4 * 48000, SDS-PAGE
tetramer
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4 * 44700, SDS-PAGE
tetramer
-
monomers are identical, crystallization experiments
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hanging drop vapor diffusion method
-
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A219C
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similar catalytic properties as the wild-type enzyme
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40
half time of thermal inactivation 161.8 s
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-20°C, at least 6 months, stable
-
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expression in Escherichia coli
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Robertson, E.F.; Reeves, H.C.
Purification and characterization of isocitrate lyase from Escherichia coli
Curr. Microbiol.
14
347-350
1987
Escherichia coli
-
brenda
Mackintosh, C.; Nimmo, H.G.
Purification and properties of Escherichia coli isocitrate lyase
Biochem. Soc. Trans.
14
320-321
1986
Escherichia coli
-
brenda
Hoyt, J.C.; Robertson, E.F.; Berlyn, K.A.; Reeves, H.C.
Escherichia coli isocitrate lyase: properties and comparisons
Biochim. Biophys. Acta
966
30-35
1988
Escherichia coli
brenda
Mackintosh, C.; Nimmo, H.G.
Purification and regulatory properties of isocitrate lyase from Escherichia coli ML308
Biochem. J.
250
25-31
1988
Escherichia coli
brenda
Zarembinski, C.M.; Hoyt, J.C.; Reeves, H.C.
Properties of isocitrate lyase from Escherichia coli K12 grown on acetate or glycolate
Curr. Microbiol.
22
65-68
1991
Escherichia coli
-
brenda
Ko, Y.H.; McFadden, B.A.
The inhibition of isocitrate lyase from Escherichia coli by glyoxylate
Curr. Microbiol.
21
313-315
1990
Escherichia coli
-
brenda
Britton, K.L.; Abeysinghe, I.S.B.; Baker, P.J.; Barynin, V.; Diehl, P.; Langridge, S.J.; McFadden, B.A.; Sedelnikova, S.E.; Stillman, T.J.; Weeradechapon, K.; Rice, D.W.
The structure and domain organization of Escherichia coli isocitrate lyase
Acta Crystallogr. Sect. D
D57
1209-1218
2001
Escherichia coli
brenda
Watanabe, S.; Takada, Y.
Amino acid residues involved in cold adaptation of isocitrate lyase from a psychrophilic bacterium, Colwellia maris
Microbiology
150
3393-3403
2004
Colwellia maris, Escherichia coli (P0A9G6), Escherichia coli
brenda
Prasad Maharjan, R.; Yu, P.L.; Seeto, S.; Ferenci, T.
The role of isocitrate lyase and the glyoxylate cycle in Escherichia coli growing under glucose limitation
Res. Microbiol.
156
178-183
2005
Escherichia coli
brenda
Ogawa, T.; Murakami, K.; Mori, H.; Ishii, N.; Tomita, M.; Yoshin, M.
Role of phosphoenolpyruvate in the NADP-isocitrate dehydrogenase and isocitrate lyase reaction in Escherichia coli
J. Bacteriol.
189
1176-1178
2007
Escherichia coli, Escherichia coli K-12 W3110
brenda
Dunn, M.F.; Ramirez-Trujillo, J.A.; Hernandez-Lucas, I.
Major roles of isocitrate lyase and malate synthase in bacterial and fungal pathogenesis
Microbiology
155
3166-3175
2009
Aspergillus fumigatus, Aspergillus nidulans, Candida albicans, Colletotrichum lagenaria, Rhodococcus equi, Cryptococcus neoformans, Escherichia coli, Leptosphaeria maculans, Pyricularia grisea, Mycobacterium tuberculosis, Mycobacterium avium, Yersinia pestis, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Sinorhizobium meliloti, Rhizobium tropici, Salmonella enterica subsp. enterica serovar Typhimurium, Yersinia enterocolitica, Paracoccidioides brasiliensis, Brucella suis, Talaromyces marneffei
brenda
Lee, Y.V.; Wahab, H.A.; Choong, Y.S.
Potential inhibitors for isocitrate lyase of Mycobacterium tuberculosis and non-M. tuberculosis a summary
BioMed Res. Int.
2015
895453
2015
Acinetobacter calcoaceticus, Ricinus communis, Escherichia coli, Pyricularia grisea, Mycobacterium tuberculosis, Neurospora crassa, Vogesella indigofera, Candida albicans (Q9P8Q7)
brenda