Information on EC 4.1.2.34 - 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
4.1.2.34
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RECOMMENDED NAME
GeneOntology No.
4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O = 2-formylbenzoate + pyruvate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
reversal of an aldol condensation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Polycyclic aromatic hydrocarbon degradation
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SYSTEMATIC NAME
IUBMB Comments
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate 2-formylbenzoate-lyase (pyruvate-forming)
Involved, with EC 1.13.11.38 (1-hydroxy-2-naphthoate 1,2-dioxygenase), in the metabolism of phenanthrene in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
86611-90-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KP7
SwissProt
Manually annotated by BRENDA team
strain KP7
SwissProt
Manually annotated by BRENDA team
gram-negative coccus, strain B156
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
(E)-2'-carboxybenzylidenepyruvate
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pH 7.0, 25°C
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0.047
2'-carboxybenzalpyruvate
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pH 7.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13
(E)-2'-carboxybenzylidenepyruvate
Nocardioides sp.
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pH 7.0, 25°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.001
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succinate as carbon source
0.029
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protocatechuate or phenanthrene as carbon source
0.12
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phthalate as carbon source
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
71000
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gel filtration
113000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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