Information on EC 4.1.2.34 - 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase

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The expected taxonomic range for this enzyme is: Bacteria

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
4.1.2.34
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O = 2-formylbenzoate + pyruvate
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
reversal of an aldol condensation
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Polycyclic aromatic hydrocarbon degradation
-
-
Microbial metabolism in diverse environments
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SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate 2-formylbenzoate-lyase (pyruvate-forming)
Involved, with EC 1.13.11.38 (1-hydroxy-2-naphthoate 1,2-dioxygenase), in the metabolism of phenanthrene in bacteria.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2'-carboxybenzalpyruvate aldolase
-
-
-
-
aldolase, 2'-carboxybenzalpyruvate
-
-
-
-
trans-2'-carboxybenzalpyruvate aldolase
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trans-2'-carboxybenzalpyruvate aldolase
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trans-2'-carboxybenzalpyruvate hydratase-aldolase
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-
-
-
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
86611-90-7
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KP7
SwissProt
Manually annotated by BRENDA team
strain KP7
SwissProt
Manually annotated by BRENDA team
gram-negative coccus, strain B156
-
-
Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
-
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
i.e. 2'-carboxybenzalpyruvate
i.e.2-carboxybenzaldehyde + pyruvate
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
i.e. 2-carboxybenzaldehyde + pyruvate
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
i.e. 2'-carboxybenzalpyruvate
i.e.2-carboxybenzaldehyde + pyruvate
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
i.e. 2-carboxybenzaldehyde + pyruvate
r
additional information
?
-
-
no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate
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-
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SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
-
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
r
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
SplaateInhibitors, Organism, Commentary, Literature,Filename
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
(E)-2'-carboxybenzylidenepyruvate
-
pH 7.0, 25°C
-
0.047
2'-carboxybenzalpyruvate
-
pH 7.0, 25°C
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
13
(E)-2'-carboxybenzylidenepyruvate
Nocardioides sp.
-
pH 7.0, 25°C
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SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.001
-
succinate as carbon source
0.029
-
protocatechuate or phenanthrene as carbon source
0.12
-
phthalate as carbon source
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.3
-
assay at
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
71000
-
gel filtration
639269
113000
-
gel filtration
648384
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
trimer
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
trimer
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
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SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature