Information on EC 4.1.2.34 - 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
4.1.2.34
-
RECOMMENDED NAME
GeneOntology No.
4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O = 2-formylbenzoate + pyruvate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
reversal of an aldol condensation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
Polycyclic aromatic hydrocarbon degradation
-
SYSTEMATIC NAME
IUBMB Comments
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate 2-formylbenzoate-lyase (pyruvate-forming)
Involved, with EC 1.13.11.38 (1-hydroxy-2-naphthoate 1,2-dioxygenase), in the metabolism of phenanthrene in bacteria.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2'-carboxybenzalpyruvate aldolase
-
-
-
-
aldolase, 2'-carboxybenzalpyruvate
-
-
-
-
phdJ
Q79EM8
;
-
trans-2'-carboxybenzalpyruvate aldolase
Q79EM8
-
trans-2'-carboxybenzalpyruvate aldolase
Q79EM8
-
-
trans-2'-carboxybenzalpyruvate hydratase-aldolase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
86611-90-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain KP7
SwissProt
Manually annotated by BRENDA team
strain KP7
SwissProt
Manually annotated by BRENDA team
gram-negative coccus, strain B156
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
-
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-, Q79EM8
i.e. 2'-carboxybenzalpyruvate
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
i.e. 2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Q79EM8
i.e. 2'-carboxybenzalpyruvate
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
i.e. 2-carboxybenzaldehyde + pyruvate
-
r
additional information
?
-
-
no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
-
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
-
the enzyme is involved in the degradation of phenanthrene
i.e.2-carboxybenzaldehyde + pyruvate
-
r
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
(E)-2'-carboxybenzylidenepyruvate
-
pH 7.0, 25°C
-
0.047
-
2'-carboxybenzalpyruvate
-
pH 7.0, 25°C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
13
-
(E)-2'-carboxybenzylidenepyruvate
-
pH 7.0, 25°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.001
-
-
succinate as carbon source
0.029
-
-
protocatechuate or phenanthrene as carbon source
0.12
-
-
phthalate as carbon source
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.3
-
-
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
71000
-
-
gel filtration
113000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
trimer
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
trimer
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-