Information on EC 4.1.2.34 - 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase

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The expected taxonomic range for this enzyme is: Bacteria

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EC NUMBER
COMMENTARY
4.1.2.34
-
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RECOMMENDED NAME
GeneOntology No.
4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase
GO:0018803
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O = 2-formylbenzoate + pyruvate
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
reversal of an aldol condensation
-
-
-
-
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PATHWAY
KEGG Link
MetaCyc Link
Polycyclic aromatic hydrocarbon degradation
00624
-
Microbial metabolism in diverse environments
01120
-
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SYSTEMATIC NAME
IUBMB Comments
(3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate 2-formylbenzoate-lyase (pyruvate-forming)
Involved, with EC 1.13.11.38 (1-hydroxy-2-naphthoate 1,2-dioxygenase), in the metabolism of phenanthrene in bacteria.
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2'-carboxybenzalpyruvate aldolase
-
-
-
-
aldolase, 2'-carboxybenzalpyruvate
-
-
-
-
phdJ
Nocardioides sp.
Q79EM8
-
639268, 648384
phdJ
Nocardioides sp. KP7
Q79EM8
;
-
trans-2'-carboxybenzalpyruvate aldolase
Nocardioides sp.
Q79EM8
-
639268
trans-2'-carboxybenzalpyruvate aldolase
Nocardioides sp. KP7
Q79EM8
-
-
trans-2'-carboxybenzalpyruvate hydratase-aldolase
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
86611-90-7
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Nocardioides sp.
strain KP7
639268, 648384
Q79EM8
SwissProt
Manually annotated by BRENDA team
Nocardioides sp. KP7
strain KP7
639268, 648384
Q79EM8
SwissProt
Manually annotated by BRENDA team
uncultured bacterium
gram-negative coccus, strain B156
639269
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
uncultured bacterium
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
639269
-
639269
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp.
-, Q79EM8
i.e. 2'-carboxybenzalpyruvate
639268
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp.
-
the enzyme is involved in the degradation of phenanthrene
648384
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp.
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
648384
i.e. 2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp. KP7
Q79EM8
i.e. 2'-carboxybenzalpyruvate
639268
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp. KP7
-
the enzyme is involved in the degradation of phenanthrene
648384
i.e.2-carboxybenzaldehyde + pyruvate
-
r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp. KP7
-
i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298
648384
i.e. 2-carboxybenzaldehyde + pyruvate
-
r
additional information
?
-
Nocardioides sp., Nocardioides sp. KP7
-
no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate
648384
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
uncultured bacterium
-
i.e. 2'-carboxybenzalpyruvate, the enzyme is involved in the degradation of phenanthrene
639269
-
639269
-
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O
2-carboxybenzaldehyde + pyruvate
show the reaction diagram
Nocardioides sp., Nocardioides sp. KP7
-
the enzyme is involved in the degradation of phenanthrene
648384
i.e.2-carboxybenzaldehyde + pyruvate
-
r
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
(E)-2'-carboxybenzylidenepyruvate
Nocardioides sp.
-
pH 7.0, 25°C
648384
-
0.047
-
2'-carboxybenzalpyruvate
uncultured bacterium
-
pH 7.0, 25°C
639269
2D-image
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TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
13
-
(E)-2'-carboxybenzylidenepyruvate
Nocardioides sp.
-
pH 7.0, 25°C
648384
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.001
-
uncultured bacterium
-
succinate as carbon source
639269
0.029
-
uncultured bacterium
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protocatechuate or phenanthrene as carbon source
639269
0.12
-
uncultured bacterium
-
phthalate as carbon source
639269
11.5
-
Nocardioides sp.
-
-
648384
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.3
-
uncultured bacterium
-
assay at
639269
7.5
-
Nocardioides sp.
-
-
648384
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TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
uncultured bacterium
-
assay at
639269
45
-
Nocardioides sp.
-
-
648384
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SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
culture condition:phenanthrene-grown cell
Nocardioides sp.
Q79EM8
-
639268, 648384
Manually annotated by BRENDA team
culture condition:phenanthrene-grown cell
Nocardioides sp. KP7
-
;
-
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
71000
-
uncultured bacterium
-
gel filtration
639269
113000
-
Nocardioides sp.
-
gel filtration
648384
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SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
trimer
Nocardioides sp.
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
648384
trimer
Nocardioides sp. KP7
-
3 * 35000, calculated from sequence; 3 * 38000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Nocardioides sp.
-
648384
partial
uncultured bacterium
-
639269
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
Nocardioides sp.
-
648384
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LINKS TO OTHER DATABASES (specific for EC-Number 4.1.2.34)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)