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Literature summary for 4.1.2.34 extracted from

  • Iwabuchi, T.; Harayama, S.
    Biochemical and genetic characterization of trans-2'-carboxybenzalpyruvate hydratase-aldolase from a phenanthrene-degrading Nocardioides strain (1998), J. Bacteriol., 180, 945-949.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.34

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nocardioides sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 (E)-2'-carboxybenzylidenepyruvate pH 7.0, 25°C Nocardioides sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 3 * 35000, calculated from sequence Nocardioides sp.
38000
-
 3 * 38000, SDS-PAGE Nocardioides sp.
113000
-
 gel filtration Nocardioides sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O Nocardioides sp. the enzyme is involved in the degradation of phenanthrene 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O Nocardioides sp. KP7 the enzyme is involved in the degradation of phenanthrene 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r

Organism

Organism UniProt Comment Textmining
Nocardioides sp. Q79EM8
-
-
Nocardioides sp. KP7 Q79EM8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardioides sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.5
-
-
 Nocardioides sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O the enzyme is involved in the degradation of phenanthrene Nocardioides sp. 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 Nocardioides sp. 2-carboxybenzaldehyde + pyruvate i.e. 2-carboxybenzaldehyde + pyruvate r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O the enzyme is involved in the degradation of phenanthrene Nocardioides sp. KP7 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
(3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 Nocardioides sp. KP7 2-carboxybenzaldehyde + pyruvate i.e. 2-carboxybenzaldehyde + pyruvate r
additional information no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate Nocardioides sp. ?
-
 ?
additional information no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate Nocardioides sp. KP7 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 38000, SDS-PAGE Nocardioides sp.
trimer 3 * 35000, calculated from sequence Nocardioides sp.

Synonyms

Synonyms Comment Organism
phdJ
-
 Nocardioides sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Nocardioides sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
 (E)-2'-carboxybenzylidenepyruvate pH 7.0, 25°C Nocardioides sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Nocardioides sp.