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Information on EC 4.1.1.33 - diphosphomevalonate decarboxylase and Organism(s) Homo sapiens and UniProt Accession P53602

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EC Tree
     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.33 diphosphomevalonate decarboxylase
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This record set is specific for:
Homo sapiens
UNIPROT: P53602 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mevalonate diphosphate decarboxylase, mevalonate pyrophosphate decarboxylase, diphosphomevalonate decarboxylase, mevalonate-5-pyrophosphate decarboxylase, mevalonate 5-diphosphate decarboxylase, pyrophosphomevalonate decarboxylase, scmdd, 5-pyrophosphomevalonate decarboxylase, scmdd1, scmdd2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mevalonate diphosphate decarboxylase
-
5-Pyrophosphomevalonate decarboxylase
-
-
-
-
Decarboxylase, pyrophosphomevalonate
-
-
-
-
Mevalonate (diphospho)decarboxylase
-
-
-
-
Mevalonate 5-diphosphate decarboxylase
-
-
-
-
mevalonate diphospho decarboxylase
-
-
Mevalonate pyrophosphate decarboxylase
-
-
-
-
mevalonate pyrophosphate decraboxylase
-
-
Mevalonate-5-pyrophosphate decarboxylase
-
-
-
-
Pyrophosphomevalonate decarboxylase
-
-
-
-
Pyrophosphomevalonic acid decarboxylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-5-diphosphomevalonate carboxy-lyase (adding ATP; isopentenyl-diphosphate-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9024-66-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
show the reaction diagram
-
-
-
?
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 5-diphosphomevalonate
ADP + phosphate + isopentenyl diphosphate + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
20 mM, required for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-fluoromevalonate 5-diphosphate
competitive inhibitor with respect to 5-diphosphomevalonate
diphosphoglycolyl proline
competitive inhibitor with respect to 5-diphosphomevalonate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0289 - 0.425
5-diphosphomevalonate
0.69 - 7.7
ATP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0022 - 4.5
5-diphosphomevalonate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000062
6-fluoromevalonate 5-diphosphate
wild type enzyme
0.0023
diphosphoglycolyl proline
wild type enzyme
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
investigation of the role of mevalonate diphospho decarboxylase in viral replication
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MVD1_HUMAN
400
0
43405
Swiss-Prot
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapour diffusion method, with 0.1 M MES buffer pH 6.5, 20% PEG 5000 MME, 0.1 M NaCl, 1 mM dithiothreitol, and 0.2 M ammonium sulfate
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N17A
mutant exhibits a 15fold inflation in Km for 5-diphosphomevalonate
R161Q
mutant exhibits an about 1000fold diminution in specific activity, while binding the fluorescent substrate analog, TNP-ATP, comparably to wild type enzyme
S162A
mutant shows reduced activity with 2fold decrease in Vm and 4 to 7fold increases in substrate Km values
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose resin chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in CV-1 cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
-
siRNAs targeting mevalonate (diphospho) decarboxylase (MVD) are found to inhibit Dengue virus replication in a stable A549 subgenomic Renilla replicon cell line (Rluc-replicon) and in naive A549 cells after dengue type 2 (DEN-2) New Guinea C (NGC) live virus infection, knock down of mevalonate (diphospho) decarboxylase can inhibit dengue viral replication
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nunez, A.; Castillo, M.; Iglesias, J.; Martinez-Cayuela, M.; Gonzales-Pacanowska, D.; Garcia-Peregrin, E.
Regulation of mevalonate 5-pyrophosphate decarboxylase in HeLa cells. Inhibition of enzymatic protein synthesis by serum lipoproteins
Int. J. Biochem. Cell Biol.
29
1037-1041
1997
Homo sapiens
Manually annotated by BRENDA team
Hogenboom, S.; Tuyp, J.J.M.; Espeel, M.; Koster, J.; Wanders, R.J.A.; Waterham, H.R.
Human mevalonate pyrophosphate decarboxylase is localized in the cytosol
Mol. Genet. Metab.
81
216-224
2004
Homo sapiens
Manually annotated by BRENDA team
Voynova, N.E.; Fu, Z.; Battaile, K.P.; Herdendorf, T.J.; Kim, J.J.; Miziorko, H.M.
Human mevalonate diphosphate decarboxylase: characterization, investigation of the mevalonate diphosphate binding site, and crystal structure
Arch. Biochem. Biophys.
480
58-67
2008
Homo sapiens (P53602), Homo sapiens
Manually annotated by BRENDA team
Rothwell, C.; Lebreton, A.; Young Ng, C.; Lim, J.Y.; Liu, W.; Vasudevan, S.; Labow, M.; Gu, F.; Gaither, L.A.
Cholesterol biosynthesis modulation regulates dengue viral replication
Virology
389
8-19
2009
Homo sapiens
Manually annotated by BRENDA team