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EC Tree
IUBMB Comments Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2. [See also EC 3.8.1.9 (R)-2-haloacid dehalogenase, EC 3.8.1.10 2-haloacid dehalogenase (configuration-inverting) and EC 3.8.1.11 2-haloacid dehalogenase (configuration-retaining)]
The taxonomic range for the selected organisms is: Burkholderia cepacia The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
2-haloacid dehalogenase, ph1421, deh99, l-2-dhlb, l-haloacid dehalogenase, (s)-2-haloacid dehalogenase, dehalogenase iva, hadl aj1, l-had, l-dexs,
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2-halo acid dehalogenase
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2-haloacid dehalogenase[ambiguous]
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2-haloacid halidohydrolase[ambiguous]
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2-haloalkanoic acid dehalogenase
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2-haloalkanoid acid halidohydrolase
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2-halocarboxylic acid dehalogenase II
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DL-2-haloacid dehalogenase [ambiguous]
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L-2-haloacid dehalogenase
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C-halide hydrolysis
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(S)-2-haloacid halidohydrolase
Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2. [See also EC 3.8.1.9 (R)-2-haloacid dehalogenase, EC 3.8.1.10 2-haloacid dehalogenase (configuration-inverting) and EC 3.8.1.11 2-haloacid dehalogenase (configuration-retaining)]
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(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
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-
?
2-chloropropionate + H2O
lactate + HCl
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?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
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?
monobromoacetate + H2O
glycolate + HBr
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?
monochloroacetate + H2O
glycolate + HCl
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-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
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-
?
2-chloropropionate + H2O
lactate + HCl
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-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
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-
-
-
?
monochloroacetate + H2O
glycolate + HCl
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-
-
-
?
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2-chloropropionate + H2O
lactate + HCl
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-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
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-
-
?
monobromoacetate + H2O
glycolate + HBr
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?
monochloroacetate + H2O
glycolate + HCl
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-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
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?
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p-mercuric chlorobenzoate
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1.13
monobromoacetate
pH 7.9, 30°C
additional information
additional information
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comparison of KM of wild-type and mutant enzymes
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additional information
additional information
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comparison of kcat of wild-type and mutant enzymes
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5.1
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the mutation from an acidic to a basic amino acid leads to an alteration in the enzyme pI valiue from 5.1 to 6.0
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Uniprot
brenda
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brenda
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HAD4_BURCE
231
0
25995
Swiss-Prot
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27000
2 * 27000, SDS-PAGE
25900
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calculated from amino acid sequence
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dimer
2 * 27000, SDS-PAGE
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D11E
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totally inactive in catalysis
D11N
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totally inactive in catalysis
D11S
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totally inactive in catalysis
D181N
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totally inactive in catalysis
N178D
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defective in catalysis
additional information
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mutant library with reduced activity
additional information
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point mutations on 29 conserved charged and/or polar residues are generated and characterized
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expression in Escherichia coli
expression in Escherichia coli and Pseudomonas putida
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Tsang, J.S.H.; Sam, L.
Cloning and characterization of a cryptic haloacid dehalogenase from Burkholderia cepacia MBA4
J. Bacteriol.
181
6003-6009
1999
Burkholderia cepacia (Q51645)
brenda
Asmara, W.; Murdiyatmo, U.; Baines, A.J.; Bull, A.T.; Hardman, D.J.
Protein engineering of the 2-haloacid halidohydrolase IVa from Pseudomonas cepacia MBA4
Biochem. J.
292
69-74
1993
Burkholderia cepacia, Burkholderia cepacia MBA4
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brenda
Pang, B.C.M.; Tsang, J.S.H.
Mutagenic analysis of the conserved residues in dehalogenase IVa of Burkholderia cepacia MBA4
FEMS Microbiol. Lett.
204
135-140
2001
Burkholderia cepacia, Burkholderia cepacia MBA4
brenda
Muridiyatmo, U.; Asmara, W.; Tsang, J.S.H.; Baines, A.J.; Bull, A.T.; Hardmann, D.J.
Molecular biology of the 2-haloacid halidohydrolas IVa from Pseudomonas cepacia MBA4
Biochem. J.
284
87-93
1992
Burkholderia cepacia
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brenda
Kurihara, T.; Liu, J.Q.; Nardi-Dei, V.; Koshikawa, H.; Esaki, N.; Soda, K.
Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues
J. Biochem.
117
1317-1322
1995
Burkholderia cepacia, Burkholderia cepacia MBA4, Moraxella sp., Moraxella sp. B, Pseudomonas putida, Pseudomonas putida AJ1, Pseudomonas putida No. 109, Pseudomonas sp., Xanthobacter autotrophicus, Xanthobacter autotrophicus GJ10
brenda