Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant library with reduced activity | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of KM of wild-type and mutant enzymes | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
i.e. Pseudomonas cepacia MBA4 | - |
Burkholderia cepacia MBA4 | - |
i.e. Pseudomonas cepacia MBA4 | - |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloropropionate + H2O | - |
Burkholderia cepacia | lactate + HCl | - |
? | |
2-chloropropionate + H2O | - |
Burkholderia cepacia MBA4 | lactate + HCl | - |
? | |
monochloroacetate + H2O | - |
Burkholderia cepacia | glycolate + HCl | - |
? | |
monochloroacetate + H2O | - |
Burkholderia cepacia MBA4 | glycolate + HCl | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of kcat of wild-type and mutant enzymes | Burkholderia cepacia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Burkholderia cepacia | the mutation from an acidic to a basic amino acid leads to an alteration in the enzyme pI valiue from 5.1 to 6.0 | - |
5.1 |