Information on EC 3.7.1.21 - 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.7.1.21
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RECOMMENDED NAME
GeneOntology No.
6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O = 3-hydroxypimeloyl-CoA
show the reaction diagram
6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O = 3-hydroxypimeloyl-CoA
show the reaction diagram
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O = 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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benzoyl-CoA degradation
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SYSTEMATIC NAME
IUBMB Comments
6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase |decyclizing||
The enzyme, which participates in the anaerobic benzoyl-CoA degradation pathway in certain organisms, catalyses the addition of one molecule of water to the double bound of 6-oxocyclohex-1-ene-1-carbonyl-CoA followed by the hydrolytic C-C cleavage of the alicyclic ring.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene bamA
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Manually annotated by BRENDA team
gene bamA
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Manually annotated by BRENDA team
Georgfuchsia sp.
gene bamA
UniProt
Manually annotated by BRENDA team
gene bamA
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Manually annotated by BRENDA team
gene bamA
SwissProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
gene bamA
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O
3-hydroxypimeloyl-CoA
show the reaction diagram
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O
3-hydroxypimeloyl-CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O
3-hydroxypimeloyl-CoA
show the reaction diagram
O87872
the enzyme participates in the anaerobic benzoyl-CoA degradation pathway
3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA
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?
6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O
3-hydroxypimeloyl-CoA
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07 - 0.115
6-oxocyclohex-1-ene-1-carbonyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
6-oxocyclohex-1-ene-1-carbonyl-CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
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in 50 mM potassium phosphate buffer (pH 7.0), at 30C
1.3
pH 7.4, 37C, substrate: 6-oxocyclohex-1-ene-1-carbonyl-CoA
2
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in 50 mM potassium phosphate buffer (pH 7.0), at 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
4 * 40000, SDS-PAGE
41900
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6 * 41900, calculated from amino acid sequence
43100
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6 * 43100, calculated from amino acid sequence
240000
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gel filtration
265000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
tetramer
4 * 40000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-chelating affinity column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli One shot BL21(DE3)pLysS cells
gene bamA, DNA and amino acid sequence determination, genotyping and phylgenetic analysis
overexpressed in Escherichia coli