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Literature summary for 3.7.1.21 extracted from
- 6-Hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, enzymes of the benzoyl-CoA pathway of anaerobic aromatic metabolism in the denitrifying bacterium Thauera aromatica (1999), Eur. J. Biochem., 263, 420-429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli | Thauera aromatica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Thauera aromatica |
| 170000 | - |
- |
Thauera aromatica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | Thauera aromatica | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera aromatica | O87872 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thauera aromatica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O = 3-hydroxypimeloyl-CoA | (1b) | Thauera aromatica | |
| 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O = 3-hydroxypimeloyl-CoA | (overall reaction) | Thauera aromatica | |
| 6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O = 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA | (1a) | Thauera aromatica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
pH 7.4, 37°C, substrate: 6-oxocyclohex-1-ene-1-carbonyl-CoA | Thauera aromatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | Thauera aromatica | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? | |
| 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | the enzyme catalyses the addition of one molecule of water to the double bound of 6-oxocyclohex-1-ene-1-carbonyl-CoA followed by the hydrolytic C-C cleavage of the alicyclic ring | Thauera aromatica | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Thauera aromatica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase | - |
Thauera aromatica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Thauera aromatica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
6-oxocyclohex-1-ene-1-carbonyl-CoA | 0.9/s per subunit | Thauera aromatica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Thauera aromatica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | Thauera aromatica |
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Release 2023.1 (January 2023)
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