Information on EC 3.6.1.8 - ATP diphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.6.1.8
-
RECOMMENDED NAME
GeneOntology No.
ATP diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + H2O = AMP + diphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP diphosphohydrolase (diphosphate-forming)
Also acts on ITP, GTP, CTP and UTP.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-25-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
snake
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
acellular slime mold
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
beta, gamma-imido ATP + H2O
AMP + imido diphosphate
show the reaction diagram
-
-
-
?
beta,gamma-methylene ATP + H2O
AMP + methylene diphosphate
show the reaction diagram
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
dATP + H2O
dAMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
ITP + H2O
IMP + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
GTP + H2O
GMP + diphosphate
show the reaction diagram
-
-
-
?
ITP + H2O
IMP + diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
no effect
Cu2+
-
no effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15-desoxybludein
-
0.065 mg/ml, 11% inhibition
-
AMP
-
slight inhibition
Caffeine
-
25% inhibition at 3 mM
cAMP
-
20% inhibition at 3 mM
eremantolide C
-
0.065 mg/ml, 17% inhibition
glaucolide A
-
0.065 mg/ml, 11% inhibition
glaucolide B
-
0.065 mg/ml, 16% inhibition
GMP
-
competitive inhibition with Ki: 0.001 mM
goyazensolide
-
0.065 mg/ml, 10% inhibition
isogoyazensolide
-
0.065 mg/ml, 11% inhibition
-
licnofolide
-
0.065 mg/ml, 22% inhibition
phosphatidylserine
-
-
thapsigargicin
-
0.065 mg/ml, 57% inhibition
thapsigargin
-
inhibits ATP hydrolysis, 70% inhibition at 0.065 mg/ml, mixed type inhibition, little or not affected by changes in free Ca2+ or Mg2+ concentrations, hydrolysis of ADP is not inhibited
UDPGlc
-
very weak
additional information
-
no inhibition by 0.065 mg/ml 15-desoxygoyazensolide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-isobutyl-1-methylxanthine
-
activation in preincubation
cAMP
-
weak activation
glucose
-
activation in preincubation
NaF
-
20 mM
NaN3
-
10 mM
oleic acid
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00032 - 2
ATP
0.0022
GTP
-
with 0.1 mM free Ca2+, pH 8
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
GMP
-
competitive inhibition
0.02
thapsigargin
-
37°C, pH 7.4, inhibition of ATP hydrolysis
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03 - 0.04
-
with ATP as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
broad optimum range
8.4 - 9
-
-
9.9
-
particular enzymes
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
20% activity at pH 6, 50% activity at pH 10
7.4 - 8.8
-
much higher activity at pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
additional information
-
18 tissues examined
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
two different localisations found
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
2 * 28000, SDS-PAGE
49000
gel filtration
56000
-
SDS-PAGE
61000
-
SDS-PAGE, soluble enzyme
127000
-
SDS-PAGE, sedimentable enzyme
240000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 28000, SDS-PAGE
tetramer
-
4 * 56000, SDS-PAGE and gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
30 min at 60°C causes no loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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