Information on EC 3.5.3.17 - Guanidinopropionase

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The expected taxonomic range for this enzyme is: Pseudomonas aeruginosa

EC NUMBER
COMMENTARY hide
3.5.3.17
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RECOMMENDED NAME
GeneOntology No.
Guanidinopropionase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-Guanidinopropanoate + H2O = beta-alanine + urea
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amidines
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SYSTEMATIC NAME
IUBMB Comments
3-Guanidinopropanoate amidinopropionase
Requires Mn2+. Also acts, more slowly, on taurocyamine and 4-guanidinobutanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
68821-77-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Guanidinopropanoate + H2O
beta-Alanine + urea
show the reaction diagram
3-guanidinopropionate + H2O
beta-alanine + urea
show the reaction diagram
4-Guanidinobutyrate + H2O
4-Aminobutyrate + urea
show the reaction diagram
Taurocyamine + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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purified native enzyme is fully active without added divalent metal, most potent activator of EDTA-treated enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-aminobutyrate
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EDTA
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reactivation by Mn2+
n-Butyrate
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PCMB
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reactivation by 2-mercaptoethanol
propionate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.8 - 108
3-guanidinopropionate
37.3 - 285
4-Guanidinobutyrate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
48.8 - 106
3-guanidinopropionate
2 - 57.6
4-Guanidinobutyrate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.28
3-guanidinopropionate
Pseudomonas aeruginosa
Q9I6K2
pH 7.9, 30°C, wild-type unmethylated GpuA
7113
1.54
4-Guanidinobutyrate
Pseudomonas aeruginosa
Q9I6K2
pH 7.9, 30°C, wild-type unmethylated GpuA
3442
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9.5
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30% of activity maximum at pH 8.0 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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x * 36000, Pseudomonas aeruginosa, SDS-PAGE
195000 - 215000
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Pseudomonas aeruginosa, gel filtration of crystalline enzyme, disc gel electrophoresis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structures are determined of Mn2+-bound GpuA (methylated), and 1,6-diaminohexane-bound GpuA (unmethylated)
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM Tris-chloride buffer, pH 8.0, 0.05 mM MnCl2, 25% glycerol, partially purified enzyme is stable for up to 5 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
mutant strain M-6, capable of utilizing taurocyamine as nitrogen source
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y157M
Km for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly increased compared to wild-type, kcat for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly decreased