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Literature summary for 3.5.3.17 extracted from
- Characterization of 3-guanidinopropionate amidinohydrolase from Pseudomonas aeruginosa and a comparative study with 4-guanidinobutyrate amidinohydrolase from another Pseudomonas (1982), Agric. Biol. Chem., 46, 1361-1367.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminobutyrate | - |
Pseudomonas aeruginosa |  |
| EDTA | reactivation by Mn2+ | Pseudomonas aeruginosa | |
| n-Butyrate | - |
Pseudomonas aeruginosa | |
| PCMB | reactivation by 2-mercaptoethanol | Pseudomonas aeruginosa | |
| propionate | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 45 | - |
3-guanidinopropionate | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | purified native enzyme is fully active without added divalent metal, most potent activator of EDTA-treated enzyme | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, Pseudomonas aeruginosa, SDS-PAGE | Pseudomonas aeruginosa |
| 195000 | 215000 | Pseudomonas aeruginosa, gel filtration of crystalline enzyme, disc gel electrophoresis | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
| Pseudomonas aeruginosa | - |
ATCC 15692 | - |
| Pseudomonas aeruginosa PAO 1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Tris-chloride buffer, pH 8.0, 0.05 mM MnCl2, 25% glycerol, partially purified enzyme is stable for up to 5 months | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-Guanidinopropanoate + H2O | - |
Pseudomonas aeruginosa | beta-Alanine + urea | - |
? | |
| 3-Guanidinopropanoate + H2O | - |
Pseudomonas aeruginosa PAO 1 | beta-Alanine + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | 3% of the activity with 3-guanidinopropanoate | Pseudomonas aeruginosa | 4-Aminobutyrate + urea | - |
? | |
| 4-Guanidinobutyrate + H2O | 3% of the activity with 3-guanidinopropanoate | Pseudomonas aeruginosa PAO 1 | 4-Aminobutyrate + urea | - |
? | |
| Taurocyamine + H2O | 11% of the activity with 3-guanidinopropanoate | Pseudomonas aeruginosa | ? | - |
? | |
| Taurocyamine + H2O | 11% of the activity with 3-guanidinopropanoate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, Pseudomonas aeruginosa, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | 30% of activity maximum at pH 8.0 and 9.5 | Pseudomonas aeruginosa |
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