Information on EC 3.5.1.B4 - acetylcitrulline deacetylase

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The expected taxonomic range for this enzyme is: Xanthomonas campestris

EC NUMBER
COMMENTARY hide
3.5.1.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
acetylcitrulline deacetylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetyl-L-citrulline + H2O = L-citrulline + acetate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
N-acetyl-L-citrulline:acetylhydrolase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N2-acetyl-L-citrulline + H2O
L-citrulline + acetate
show the reaction diagram
N2-acetyl-L-ornithine + H2O
L-ornithine + acetate
show the reaction diagram
-
in vitro ArgE deacetylates both N2-acetylcitrulline and N2-acetylornithine with almost equal velocity and shows apparent first-order rate constants
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N2-acetyl-L-citrulline + H2O
L-citrulline + acetate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
bound to active site, coordinated by His72, Asp103, Glu155, and one water molecule
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00048
N2-acetyl-L-citrulline
Xanthomonas campestris
-
pH 7.0, 37°C
-
0.00049
N2-Acetyl-L-ornithine
Xanthomonas campestris
-
pH 7.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
2 * 40000, crystal structure analysis, dimeric structure suggested also from comparison with similar proteins
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tag free protein
-
His-tag free protein, hanging drop vapor diffusion method
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)
-
expressed as His-tag fusion protein in Escherichia coli BL21(DE3), expressed as selenomethionine substituted protein in Escherichia coli B834(DE3)
-
expression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
-
possible target for drugs