Literature summary for 3.5.1.B4 extracted from

Shi, D.; Yu, X.; Roth, L.; Morizono, H.; Hathout, Y.; Allewell, N.M.; Tuchman, M.
Expression, purification, crystallization and preliminary X-ray crystallographic studies of a novel acetylcitrulline deacetylase from Xanthomonas campestris (2005), Acta Crystallogr. Sect. F, 61, 676-679.

Search for more literature for 3.5.1.B4

Application

Application	Comment	Organism
drug development	possible target for drugs	Xanthomonas campestris

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3), expressed as selenomethionine substituted protein in Escherichia coli B834(DE3)	Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment)	Organism
His-tag free protein	Xanthomonas campestris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	2 * 40000, crystal structure analysis, dimeric structure suggested also from comparison with similar proteins	Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-citrulline + H2O	Xanthomonas campestris	involved in an alternative pathway for arginine synthesis	L-citrulline + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris	-	strain ATCC 33913D	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein using His-tag	Xanthomonas campestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2-acetyl-L-citrulline + H2O	-	Xanthomonas campestris	L-citrulline + acetate	-	?
N2-acetyl-L-citrulline + H2O	involved in an alternative pathway for arginine synthesis	Xanthomonas campestris	L-citrulline + acetate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 40000, crystal structure analysis, dimeric structure suggested also from comparison with similar proteins	Xanthomonas campestris

Synonyms

Synonyms	Comment	Organism
argE' gene product	-	Xanthomonas campestris

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay	Xanthomonas campestris

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Release 2023.1 (January 2023)